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β-Actin,beta Actin Antibody

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  • ¥900
  • affinity
  • AF7018
  • 2025年10月30日
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    • 详细信息
    • 文献和实验
    • 技术资料
    • 抗体名

      beta Actin Antibody

    • 抗体英文名

      β-Actin

    • 宿主

    • 库存

      大量

    • 保质期

      12个月

    • 抗原来源

      其它

    • 供应商

      上海研卉生物科技有限公司

    • 是否单克隆

      多克隆

    • 规格

      50ul

    β-Actin,beta Actin Antibody

    名 称:
    beta Actin 抗体
    货 号:
    AF7018
    来 源:
    Rabbit
    应 用:
    WB,IHC,IF/ICC
    反 应:
    Human, Mouse, Rat, Pig, Zebrafish, Dog, Monkey, Fish
    预 测:
    Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Chicken(100%), Xenopus(100%)
    蛋白号:
    P60709
    分子量:
    43kDa
    浓 度:
    1mg/ml
    Alternative Names:Expand▼

    ACTB; Actin; cytoplasmic 1; Beta-actin; Beta actin; BRWS1; β actin;b actin; Actin beta; Beta cytoskeletal actin; PS1TP5-binding protein 1; PS1TP5BP1;

    Applications:

    WB 1:3000-1:20000, IHC 1:200, IF/ICC 1:100-1:500, ELISA(peptide) 1:20000-1:40000

    Reactivity:

    Human, Mouse, Rat, Pig, Zebrafish, Dog, Monkey, Fish

    Predicted Reactivity:

    Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Chicken(100%), Xenopus(100%)

    Source:

    Rabbit

    Clonality:

    Polyclonal

    Purification:

    The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

    Specificity:

    Beta actin antibody detects endogenous levels of total Beta actin.

    Format:

    Liquid

    Concentration:

    1mg/ml

    参考文献:

    Reference Citations:

    1). Tang H et al. circKIF4A acts as a prognostic factor and mediator to regulate the progression of triple-negative breast cancer. Mol Cancer 2019 Feb 11;18(1):23 (PubMed: 30744636) [IF=10.679]

    2). Zhou P et al. Multifunctional nanoparticles based on a polymeric copper chelator for combination treatment of metastatic breast cancer. Biomaterials 2019 Jan 4;195:86-99 (PubMed: 30623789) [IF=10.273]

    3). Yi S et al. Tcf12, A Member of Basic Helix-Loop-Helix Transcription Factors, Mediates Bone Marrow Mesenchymal Stem Cell Osteogenic Differentiation In Vitro and In Vivo. Stem Cells 2017 Feb;35(2):386-397 (PubMed: 27574032) [IF=5.614]
    产品细节图片1

    Application: WB Species:mouse; Sample:Not available

    Figure 5. Down regulation of Tcf12 accelerate and potentiate bone repair in vivo. (A‐B) Quantification of Alp activity at day 14 in U‐0126 and LDN‐193189 treated shTcf12 and scrambled groups.


    4). Zhao YR et al. HEG1 indicates poor prognosis and promotes hepatocellular carcinoma invasion, metastasis, and EMT by activating Wnt/β-catenin signaling. Clin Sci (Lond) 2019 Jul 5 (PubMed: 31278131) [IF=5.237]

    5). Zhou H et al. miR-506 enhances the sensitivity of human colorectal cancer cells to oxaliplatin by suppressing MDR1/P-gp expression. Cell Prolif 2017 Jun;50(3) (PubMed: 28217977) [IF=5.039]
    产品细节图片2

    Application: WB Species:human; Sample:Not available

    FIGURE 3  miR-506 down-regulated MDR1/P-gp expression in HCT116-OxR. A, The mRNA level of MDR1 was decreased after transfection with the miR-506 mimic of the relative chemoresistant genes as demonstrated by qRT-PCR. B, The protein level of MDR1 was decreased after transfection with the miR-506 mimic of the relative chemoresistant proteins as demonstrated by Western blot. C, Expression of P-gp detected by immunofluorescence staining.HCT116-OxR-miR-506 cells showed low levels of fluorescent staining of P-gp, whereas maximal staining of P-gp was observed in HCT116-OxR cells, readily distinguished from background. Zoom: 200×. *P<.05)


    6). Huang H et al. Nutritional Preconditioning of Apigenin Alleviates Myocardial Ischemia/Reperfusion Injury via the Mitochondrial Pathway Mediated by Notch1/Hes1. Oxid Med Cell Longev 2019 Mar 20;2019:7973098 (PubMed: 31015891) [IF=4.868]

    7). Luan X et al. Anisamide-targeted PEGylated gold nanoparticles designed to target prostate cancer mediate: Enhanced systemic exposure of siRNA, tumour growth suppression and a synergistic therapeutic response in combination with paclitaxel in mice. Eur J Pharm Biopharm 2019 Feb 16 (PubMed: 30779980) [IF=4.708]

    8). Ding L et al. Noncoding transcribed ultraconserved region (T-UCR) UC.48+ is a novel regulator of high-fat diet induced myocardial ischemia/reperfusion injury. J Cell Physiol 2019 Jun;234(6):9849-9861 (PubMed: 30417395) [IF=4.522]

    9). He F et al. Irradiation-induced osteocyte damage promotes HMGB1-mediated osteoclastogenesis in vitro. J Cell Physiol 2019 Feb 20 (PubMed: 30786022) [IF=4.522]

    10). Kong Y et al. CircPLK1 sponges miR-296-5p to facilitate triple-negative breast cancer progression. Epigenomics 2019 Jul 24 (PubMed: 31337246) [IF=4.404]

    11). Li CL et al. Comparison of anti-inflammatory effects of berberine, and its natural oxidative and reduced derivatives from Rhizoma Coptidis in vitro and in vivo. Phytomedicine 2019 Jan;52:272-283 (PubMed: 30599908) [IF=4.180]

    12). Yang Z et al. CCL2/CCR2 Axis Promotes the Progression of Salivary Adenoid Cystic Carcinoma via Recruiting and Reprogramming the Tumor-Associated Macrophages. Front Oncol 2019 Apr 9;9:231 (PubMed: 31024838) [IF=4.137]

    13). Xiao X et al. The miR-34a-LDHA axis regulates glucose metabolism and tumor growth in breast cancer. Sci Rep 2016 Feb 23;6:21735 (PubMed: 26902416) [IF=4.011]
    产品细节图片3

    Application: WB Species:human; Sample:breast cancer


    14). Lu M et al. CTGF Triggers Rat Astrocyte Activation and Astrocyte-Mediated Inflammatory Response in Culture Conditions. Inflammation 2019 Jun 10 (PubMed: 31183597)

    15). Yong Wang et al. The Role of YB1 in Renal Cell Carcinoma Cell Adhesion. Int J Med Sci 2018; 15(12):1304-1311

    16). Zhang W et al. Effects of microencapsulated olfactory ensheathing cell transplantation on neuropathic pain and P2X7 receptor expression in the L4-5 spinal cord segment. Neurosci Lett 2019 Feb 9 (PubMed: 30753909)

    17). Zhuoqi Liu et al. Effect of targeted mammalian sterile 20-like kinase 1 regulation on proliferation and apoptosis of SW480 colorectal cancer cells. Biomedical Research 2017 Jun 8;28(15)

    18). Jia Y et al. Overexpression of CD59 inhibits apoptosis of T-acute lymphoblastic leukemia via AKT/Notch1 signaling pathway. Cancer Cell Int 2019 Jan 8;19:9 (PubMed: 30636930)

    19). Yang L et al. Angiogenic function of astragaloside IV in rats with myocardial infarction occurs via the PKD1-HDAC5-VEGF pathway. Exp Ther Med 2019 Apr;17(4):2511-2518 (PubMed: 30906439)

    20). Wang X et al. Autophagy enhanced antitumor effect in K562 and K562/ADM cells using realgar transforming solution. Biomed Pharmacother 2018 Feb;98:252-264 (PubMed: 29272786)

    21). Yu Gong;Meijiao Song;Jianguo Zhao;Jianping Xiong;Xiaojun Xiang;Ling Zhang;Feng Yu;Jun Chen;Miao Feng;Zhengyu Zhan et al. Targeting CUL4A inhibits HCT-116 colon cancer cell proliferation and invasion in vitro and in vivo. Oncology Letters

    22). Xie J et al. circEPSTI1 regulates ovarian cancer progression via decoying miR-942. J Cell Mol Med 2019 Mar 19 (PubMed: 30887698)

    23). Peng XR et al. Supplementation exogenous bile acid improved growth and intestinal immune function associated with NF-κB and TOR signalling pathways in on-growing grass carp (Ctenopharyngodon idella): Enhancement the effect of protein-sparing by dietary lipid. Fish Shellfish Immunol 2019 Jun 26;92:552-569 (PubMed: 31252043)

    24). Li Z et al. The role of PGC-1α and MRP1 in lead-induced mitochondrial toxicity in testicular Sertoli cells. Toxicology 2016 Apr 29;355-356:39-48 (PubMed: 27236077)
    产品细节图片4

    Application: WB Species:mouse; Sample:Not available

    Fig. 6. Role of MRP1 on lead-induced mitochondrial toxicity. (A) Expression of MRP1 in the mitochondria of Sertoli cells with various concentrations of lead acetate. (B) Expression of MRP1 in the mitochondria of Sertoli cells with continuous time exposure to lead acetate (20mM). (C) Expression of MRP1 in the mitochondria of the TM4 cells and MRP1()TM4 cells. (D) Transport activity of MRP1 in the mitochondria of the TM4 cells and MRP1()TM4 cells. (E) The accumulation of lead in the mitochondria of TM4 cells and MRP1()TM4 cells. Data represent mean SD of at least three independent experiments (*P < 0.05, **P < 0.01 vs. control).


    25). Keren Wu et al. Antitumor effect of ginsenoside Rg3 on gallbladder cancer by inducing endoplasmic reticulum stress‑mediated apoptosis in vitro and in vivo. ONCOL LETT 2018 Aug;0:0-0

    26). Wu M et al. Essential oils from Inula japonica and Angelicae dahuricae enhance sensitivity of MCF-7/ADR breast cancer cells to doxorubicin via multiple mechanisms. J Ethnopharmacol 2016 Mar 2;180:18-27 (PubMed: 26795076)

    27). Locatelli FM et al. Resveratrol-loaded nanoemulsion prevents cognitive decline after abdominal surgery in aged rats. J Pharmacol Sci 2018 Aug;137(4):395-402 (PubMed: 30196020)

    28). Zhu L et al. miR-199b-5p Regulates Immune-Mediated Allograft Rejection after Lung Transplantation Through the GSK3β and NF-κB Pathways. Inflammation 2018 Aug;41(4):1524-1535 (PubMed: 29779167)

    29). Ge QD et al. Differential Expression of miRNAs in the Hippocampi of Offspring Rats Exposed to Fluorine Combined with Aluminum during the Embryonic Stage and into Adulthood. Biol Trace Elem Res 2018 Jul 22 (PubMed: 30033483)

    30). et al. Effects and Mechanisms of Vitamin C Post-Conditioning on Platelet Activation after Hypoxia/Reoxygenation.

    31). Wu X et al. Antioxidative and Anti-Inflammatory Effects of Water Extract of Acrostichum aureum Linn. against Ethanol-Induced Gastric Ulcer in Rats. Evid Based Complement Alternat Med 2018 Dec 12;2018:3585394 (PubMed: 30643529)

    32). Plaza A et al. Inflammatory stress and altered angiogenesis evoked by very high-fat diets in mouse liver. Endocrinol Diabetes Nutr 2019 Mar 1 (PubMed: 30833154)

    33). Huang X et al. PDL1 And LDHA act as ceRNAs in triple negative breast cancer by regulating miR-34a. J Exp Clin Cancer Res 2017 Sep 15;36(1):129 (PubMed: 28915924)

    34). Li L et al. MiR-93-5p promotes gastric cancer-cell progression via inactivation of the Hippo signaling pathway. Gene 2018 Jan 30;641:240-247 (PubMed: 29045821)

    35). Huang H et al. Astragaloside IV protects cardiomyocytes from anoxia/reoxygenation injury by upregulating the expression of Hes1 protein. Can J Physiol Pharmacol 2016 May;94(5):542-53 (PubMed: 27070866)

    36). Huang YF et al. Therapeutic effect of Brucea javanica oil emulsion on experimental Crohn's disease in rats: Involvement of TLR4/ NF-κB signaling pathway. Biomed Pharmacother 2019 Mar 19;114:108766 (PubMed: 30901719)

    37). Wu CZ et al. HMGB1/RAGE axis mediates the apoptosis, invasion, autophagy, and angiogenesis of the renal cell carcinoma. Onco Targets Ther 2018 Aug 1;11:4501-4510 (PubMed: 30122942)

    38). Ren W et al. Arginine inhibits the malignant transformation induced by interferon-gamma through the NF-κB-GCN2/eIF2α signaling pathway in mammary epithelial cells in vitro and in vivo. Exp Cell Res 2018 Jul 15;368(2):236-247 (PubMed: 29746817)

    39). Zhong JR et al. Phytic acid disrupted intestinal immune status and suppressed growth performance in on-growing grass carp (Ctenopharyngodon idella). Fish Shellfish Immunol 2019 Jun 24;92:536-551 (PubMed: 31247320)

    40). Han H et al. Lamotrigine attenuates cerebral ischemia-induced cognitive impairment and decreases β-amyloid and phosphorylated tau in the hippocampus in rats. Neuroreport 2015 Aug 19;26(12):723-7 (PubMed: 26164461)
    产品细节图片5

    Application: WB Species:rat; Sample:rat

    LTG decreased Aβ1–42 and phosphorylated tau protein levels in the ipsilateral hippocampus of MCAO rats. (a) Immunoblotting photographs of Aβ1–42, Tau-5, and AT8. (b–d) Quantitative analysis of immunoreactivity of Aβ1–42 (b), Tau-5 (c), and AT8 (d). (e) Immunofluorescence of the hippocampal CA1 zone for AT8. Scale bar=50 μm. *Means sham versus another group, *P<0.05, **P< 0.01, ***P<0.001; † LTG 20 versus vehicle, † P<0.05, †††P<0.001; ‡ LTG 40 versus vehicle, ‡‡P< 0.01, ‡‡‡P


    41). Zhang D et al. Wnt-3a alleviates neuroinflammation after ischemic stroke by modulating the responses of microglia/macrophages and astrocytes. Int Immunopharmacol 2019 Jul 16;75:105760 (PubMed: 31323530)

    42). Cai W et al. Uric Acid Induces Endothelial Dysfunction by Activating the HMGB1/RAGE Signaling Pathway. Biomed Res Int 2017;2017:4391920 (PubMed: 28116308)
    产品细节图片6

    Application: WB Species:human; Sample:Not available


    43). Zhang G et al. Acute stimulatory effect of tumor necrosis factor on the basolateral 50 pS K channels in the thick ascending limb of the rat kidney. Mol Med Rep 2018 Sep 10 (PubMed: 30221721)

    44). Zheng L et al. Selenium deficiency impaired immune function of the immune organs in young grass carp (Ctenopharyngodon idella). Fish Shellfish Immunol 2018 Jun;77:53-70 (PubMed: 29559270)

    45). Gong Y et al. CUL4A promotes cell invasion in gastric cancer by activating the NF-κB signaling pathway. Biologics 2017 Apr 12;11:45-53 (PubMed: 28442889)
    产品细节图片7

    Application: WB Species:human; Sample:HGC27

    Figure 4 CUL4A and NF-κB were overexpressed in GC tissues. Notes: (A) Representative images of gastric tumor tissues showing concordant positive staining of CUL4A and NF-κB in the same sample (200×). (B) Western blot analysis of CUL4A and NF-κB expressions in three paired primary GC and adjacent noncancerous tissue samples. These three patients were all diagnosed stage III. (C) CUL4A expression scores and NF-κB expression scores in 50 GC samples revealed that CUL4A expression positively correlated with NF-κB expression via correlation analysis


    46). Zhang H et al. Crocetin inhibits PDGF-BB-induced proliferation and migration of retinal pigment epithelial cells. Eur J Pharmacol 2018 Nov 2 (PubMed: 30395849)

    47). et al. Protective effect of Schisandra chinensis total lignans on acute alcoholic-induced liver injury related to inhibiting CYP2E1 activation and activating the Nrf2/ARE signaling pathway.

    48). Huikun Xu et al. Chemerin promotes the viability and migration of human placental microvascular endothelial cells and activates MAPK/AKT signaling . Int J Clin Exp Med 2018;11(2):721-727

    49). et al. CircABCC2 Regulates Hepatocellular Cancer Progression by Decoying MiR-665.

    50). Yong Wang et al. The Role of YB1 in Renal Cell Carcinoma Cell Adhesion. Int J Med Sci 2018; 15(12):1304-1311

    51). Hui-Fang Wang et al. Crocetin inhibits the proliferation, migration and TGF-β 2-induced epithelial-mesenchymal transition of retinal pigment epithelial cells . EUR J PHARMACOL 2017 Nov;815:391-398

    52). Han S et al. High CCL7 expression is associated with migration, invasion and bone metastasis of non-small cell lung cancer cells. Am J Transl Res 2019 Jan 15;11(1):442-452 (PubMed: 30788000)

    53). Xin Y et al. Versatile Pt NCs-based chemotherapeutic agents significantly induce the apoptosis of cisplatin-resistant non-small cell lung cancer. Biochem Biophys Res Commun 2019 Mar 15 (PubMed: 30885437)

    54). Feng Y et al. Bone marrow stromal cells promote neuromotor functional recovery, via upregulation of neurotrophic factors and synapse proteins following traumatic brain injury in rats. Mol Med Rep 2017 Jul;16(1):654-660 (PubMed: 28560414)

    55). Ren WB et al. Interferon-γ regulates cell malignant growth via the c-Abl/HDAC2 signaling pathway in mammary epithelial cells. J Zhejiang Univ Sci B 2019 Jan (PubMed: 30614229)

    56). et al. Effects of Sancai Lianmei Particle on autophagy and apoptosis in testes of diabetic mice via the Nrf2/HO-1 pathway.

    57). Tian YM et al. Anti-diabetes effect of chronic intermittent hypobaric hypoxia through improving liver insulin resistance in diabetic rats. Life Sci 2016 Apr 1;150:1-7 (PubMed: 26883978)

    58). Chen G et al. SOSTDC1 inhibits bone metastasis in non-small cell lung cancer and may serve as a clinical therapeutic target. Int J Mol Med 2018 Oct 10 (PubMed: 30320379)

    59). Wang J et al. Interfering Expression of Chimeric Transcript SEPT7P2-PSPH Promotes Cell Proliferation in Patients with Nasopharyngeal Carcinoma. J Oncol 2019 Apr 1;2019:1654724 (PubMed: 31057610)

    60). Zhu L et al. miR-199b-5p Regulates Immune-Mediated Allograft Rejection after Lung Transplantation Through the GSK3β and NF-κB Pathways. Inflammation 2018 Aug;41(4):1524-1535 (PubMed: 29779167)

    61). Wen L et al. Increased expression of long noncoding RNA GAS6-AS2 promotes proliferation and inhibits apoptosis of melanoma cells via upregulating GAS6 expression. IUBMB Life 2019 Jun 4 (PubMed: 31162889)

    62). Lv C et al. Long-Term DL-3-n-Butylphthalide Treatment Alleviates Cognitive Impairment Correlate With Improving Synaptic Plasticity in SAMP8 Mice. Front Aging Neurosci 2018 Jul 5;10:200 (PubMed: 30026693)

    63). Hu Y et al. Expression and clinical relevance of SPOPL in medulloblastoma. Oncol Lett 2017 Sep;14(3):3051-3056 (PubMed: 28928843)
    产品细节图片8

    Application: WB Species:human; Sample:Not available


    64). Chen Z et al. Lower Expression of Gelsolin in Colon Cancer and Its Diagnostic Value in Colon Cancer Patients. J Cancer 2019 Jan 30;10(5):1288-1296 (PubMed: 30854138)

    65). Deng J et al. MicroRNA-506 inhibits gastric cancer proliferation and invasion by directly targeting Yap1. Tumour Biol 2015 Sep;36(9):6823-31 (PubMed: 25846731)

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