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- 详细信息
- 技术资料
- 抗体名:
G蛋白激活内向钾通道5抗体
- 抗体英文名:
Anti-KCNJ5
- 靶点:
详见说明书
- 浓度:
1mg/1ml
- 应用范围:
WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 Flow-Cyt=1:20-100 ICC=1:100-500 IF=1:50-200
- 宿主:
详见说明书
- 供应商:
上海谷研
- 库存:
55
- 级别:
详见说明书
- 目录编号:
详见说明书
- 抗原来源:
Rabbit
- 保质期:
详见说明书
- 适应物种:
详见说明书
- 标记物:
详见说明书
- 克隆性:
多克隆
- 保存条件:
Store at -20 °C
- 形态:
详见说明书
- 亚型:
IgG
- 免疫原:
KLH conjugated synthetic peptide derived from human KCNJ5
- 规格:
0.2ml/200μg
G蛋白激活内向钾通道5抗体0.2ml/200μg抗体的多样性:
抗体的异质性。抗体的组成极为复杂,是由成千上万、多种多样的免疫球蛋白(Ig)分子所组成。这些Ig分子在形状、大小、结构以及氨基酸的组成和排列上,既相似,又有差别。由于有差别,它们的电泳活性就有很大的变化。
因为抗体具有与抗原决定簇相对应的结合部位(抗原结合簇),所以抗体与抗原的结合具有特异性。另一方面,抗体本身是一种蛋白质,具有本身的氨基酸组成、排列和立体结构,对异种动物来说,它又是抗原。各类Ig都具有可用血清学方法检出的抗原特异性,它们表现出不同的血清学类型。
英文名称 Anti-KCNJ5
中文名称 G蛋白激活内向钾通道5抗体0.2ml/200μg
别 名 inwardly rectifying subfamily J member 5; Cardiac ATP sensitive potassium channel; Cardiac inward rectifier; CIR; G protein activated inward rectifier potassium channel 4; G protein-activated inward rectifier potassium channel 4; GIRK 4; GIRK-4; GIRK4; Heart KATP channel; Inward rectifier K(+) channel Kir3.4; Inward rectifier K+ channel KIR3.4; IRK-4; IRK5_HUMAN; KATP 1; KATP-1; KATP1; KCNJ 5; Kcnj5; KIR 3.4; KIR3.4; Potassium channel; Potassium channel inwardly rectifying subfamily J member 5; Potassium inwardly rectifying channel J5; Potassium inwardly rectifying channel subfamily J member 5.
浓 度 1mg/1ml
规 格 0.2ml/200μg
抗体来源 Rabbit
克隆类型 polyclonal
交叉反应 Human, Mouse, Rat, Chicken, Pig, Cow, Horse, Rabbit, Sheep
产品类型 一抗
研究领域 肿瘤 心血管 神经生物学 通道蛋白 G蛋白信号
蛋白分子量 predicted molecular weight:48kDa
性 状 Lyophilized or Liquid
免 疫 原 KLH conjugated synthetic peptide derived from human KCNJ5
亚 型 IgG
纯化方法 affinity purified by Protein A
储 存 液 Preservative: 15mM Sodium Azide, Constituents: 1% BSA, 0.01M PBS, pH 7.4
G蛋白激活内向钾通道5抗体0.2ml/200μg产品应用 WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 Flow-Cyt=1:20-100 ICC=1:100-500 IF=1:50-200
(石蜡切片需做抗原修复)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
保存条件 Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
Important Note This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
产品介绍 Potassium channels are present in most mammalian cells, where they participate in a wide range of physiologic responses. The protein encoded by this gene is an integral membrane protein and inward-rectifier type potassium channel. The encoded protein, which has a greater tendency to allow potassium to flow into a cell rather than out of a cell, is controlled by G-proteins. It may associate with two other G-protein-activated potassium channels to form a heteromultimeric pore-forming complex. [provided by RefSeq, Jul 2008].
Function :
This potassium channel is controlled by G proteins. Inward rectifier potassium channels are characterized by a greater tendency to allow potassium to flow into the cell rather than out of it. Their voltage dependence is regulated by the concentration of extracellular potassium; as external potassium is raised, the voltage range of the channel opening shifts to more positive voltages. The inward rectification is mainly due to the blockage of outward current by internal magnesium. Can be blocked by external barium.
Subunit :
May associate with GIRK1 and GIRK2 to form a G-protein-activated heteromultimer pore-forming unit. The resulting inward current is much larger (By similarity).
Subcellular Location :
Membrane; Multi-pass membrane protein.
G蛋白激活内向钾通道5抗体0.2ml/200μg具有全、新、优、品、好四大特点:
全:公司提供上万种产品,涵盖了生物试剂,elisa试剂盒,标准品,培养基,原装耗材,抗体、培养基、ATCC细胞等,基本上各种科研所需产品在我司都能找到。
新:产品更新速度较快,基本上每周都有新产品出现。
优:产品质量好,投诉比较少。
好:我公司具有优质的技术团队,产品一旦售出,实验过程中遇到困难可提供在线技术咨询。使您使用产品时没有任何的后顾之忧。
G蛋白激活内向钾通道5抗体0.2ml/200μg实验步骤
① 滴加0.01mol/L,pH7.4的PBS于待检标本片上,10min后弃去,使标本保持一定湿度。
② 滴加适当稀释的荧光标记的抗体溶液,使其完全覆盖标本,置于有盖搪瓷盒内,保温一定时间(参考:30min)。
③ 取出玻片,置玻片架上,先用0.01mol/L,pH7.4的PBS冲洗后,再按顺序过0.01mol/L,pH7.4的PBS三缸浸泡,每缸3-5 min,不时振荡。
④ 取出玻片,用滤纸吸去多余水分,但不使标本干燥,加一滴缓冲甘油,以盖玻片覆盖。
⑤ 立即用荧光显微镜观察。观察标本的特异性荧光强度,一般可用“+”表示:
(-)无荧光;(±)极弱的可疑荧光;(+)荧光较弱,但清楚可见;(++)荧光明亮;(+++ --++++)荧光闪亮。待检标本特异性荧光染色强度达“++”以上,而各种对照显示为(±)或(-),即可判定为阳性。
G蛋白激活内向钾通道5抗体0.2ml/200μg现货供应,货期短,为您提供售前、售中、售后服务,保证实验结果,无效可以免费退换,提供免费代测服务,产品效价高、种类全、价格实惠 ,欢迎来电咨询!
CD163L1 CD163蛋白样1抗体 特价促销
CDAN1 先天性红细胞生成异常性贫血蛋白1抗体 特价促销
CDK2AP2 细胞周期素依赖激酶2相关蛋白2抗体 特价促销
CEACAM21 癌胚抗原相关细胞粘附分子21抗体 特价促销
CDKAL1 周期素依赖性激酶5调节蛋白1样蛋白1抗体 特价促销
CEACAM16/CEAL2 癌胚抗原相关细胞粘附分子16抗体 特价促销
CEACAM19/CEAL1 癌胚抗原相关细胞粘附分子19抗体 特价促销
ADCK5 ADCK5蛋白抗体 特价促销
ITGB1BP2/CHORDC3 整合素β1结合蛋白2抗体 特价促销
CLDND2 膜蛋白CLDND2抗体 特价促销
G蛋白激活内向钾通道5抗体0.2ml/200μgELISA Kit for Human Sodium/calcium exchanger 1 现货供应 0.156-10 ng/mL
ELISA Kit for Human Sodium-dependent serotonin transporter 现货供应 31.2-2000 pg/mL
ELISA Kit for Human Solute carrier family 12 member 1 现货供应 0.312-20 ng/mL
ELISA Kit for Human Solute carrier family 2, facilitated glucose transporter member 1 现货供应 0.156-10 ng/mL
ELISA Kit for Human Solute carrier family 2, facilitated glucose transporter member 3 现货供应 0.156-10 ng/mL
ELISA Kit for Human Solute carrier family 2, facilitated glucose transporter member 4 现货供应 0.156-10 ng/mL
ELISA Kit for Human Solute carrier family 40 member 1 现货供应 0.156-10 ng/mL
ELISA Kit for Human Somatoliberin 现货供应 0.39-25 ng/mL
ELISA Kit for Human Somatostatin 现货供应 6.25-400 pg/mL
ELISA Kit for Human Somatostatin receptor type 2 现货供应 78-5000 pg/mL
CD163L1 CD163蛋白样1抗体 特价促销
CDAN1 先天性红细胞生成异常性贫血蛋白1抗体 特价促销
CDK2AP2 细胞周期素依赖激酶2相关蛋白2抗体 特价促销
CEACAM21 癌胚抗原相关细胞粘附分子21抗体 特价促销
CDKAL1 周期素依赖性激酶5调节蛋白1样蛋白1抗体 特价促销
CEACAM16/CEAL2 癌胚抗原相关细胞粘附分子16抗体 特价促销
CEACAM19/CEAL1 癌胚抗原相关细胞粘附分子19抗体 特价促销
ADCK5 ADCK5蛋白抗体 特价促销
ITGB1BP2/CHORDC3 整合素β1结合蛋白2抗体 特价促销
CLDND2 膜蛋白CLDND2抗体 特价促销
注意事项
1)对荧光标记的抗体的稀释,要保证抗体的蛋白有一定的浓度,一般稀释度不应超过1:20,抗体浓度过低,会导致产生的荧光过弱,影响结果的观察。
2)染色的温度和时间需要根据各种不同的标本及抗原而变化,染色时间可以从10 min到数小时,一般30 min已足够。染色温度多采用室温(25℃左右),高于37℃可加强染色效果,但对不耐热的抗原(如流行性乙型脑炎病毒)可采用0-2℃的低温,延长染色时间。低温染色较37℃30 min效果好的多。
3)为了保证荧光染色的正确性,首次试验时需设置下述对照,以排除某些非特异性荧光染色的干扰。
① 标本自发荧光对照:标本加1-2滴0.01mol/L,pH7.4的PBS。
② 特异性对照(抑制试验):标本加未标记的特异性抗体,再加荧光标记的特异性抗体。
③ 阳性对照:已知的阳性标本加荧光标记的特异性抗体。
如果标本自发荧光对照和特异性对照呈无荧光或弱荧光,阳性对照和待检标本呈强荧光,则为特异性阳性染色。
4)一般标本在高压汞灯下照射超过3min,就有荧光减弱现象,经荧光染色的标本zui好在当天观察,随着时间的延长,荧光强度会
抗体的异质性。抗体的组成极为复杂,是由成千上万、多种多样的免疫球蛋白(Ig)分子所组成。这些Ig分子在形状、大小、结构以及氨基酸的组成和排列上,既相似,又有差别。由于有差别,它们的电泳活性就有很大的变化。
因为抗体具有与抗原决定簇相对应的结合部位(抗原结合簇),所以抗体与抗原的结合具有特异性。另一方面,抗体本身是一种蛋白质,具有本身的氨基酸组成、排列和立体结构,对异种动物来说,它又是抗原。各类Ig都具有可用血清学方法检出的抗原特异性,它们表现出不同的血清学类型。
英文名称 Anti-KCNJ5
中文名称 G蛋白激活内向钾通道5抗体0.2ml/200μg
别 名 inwardly rectifying subfamily J member 5; Cardiac ATP sensitive potassium channel; Cardiac inward rectifier; CIR; G protein activated inward rectifier potassium channel 4; G protein-activated inward rectifier potassium channel 4; GIRK 4; GIRK-4; GIRK4; Heart KATP channel; Inward rectifier K(+) channel Kir3.4; Inward rectifier K+ channel KIR3.4; IRK-4; IRK5_HUMAN; KATP 1; KATP-1; KATP1; KCNJ 5; Kcnj5; KIR 3.4; KIR3.4; Potassium channel; Potassium channel inwardly rectifying subfamily J member 5; Potassium inwardly rectifying channel J5; Potassium inwardly rectifying channel subfamily J member 5.
浓 度 1mg/1ml
规 格 0.2ml/200μg
抗体来源 Rabbit
克隆类型 polyclonal
交叉反应 Human, Mouse, Rat, Chicken, Pig, Cow, Horse, Rabbit, Sheep
产品类型 一抗
研究领域 肿瘤 心血管 神经生物学 通道蛋白 G蛋白信号
蛋白分子量 predicted molecular weight:48kDa
性 状 Lyophilized or Liquid
免 疫 原 KLH conjugated synthetic peptide derived from human KCNJ5
亚 型 IgG
纯化方法 affinity purified by Protein A
储 存 液 Preservative: 15mM Sodium Azide, Constituents: 1% BSA, 0.01M PBS, pH 7.4
G蛋白激活内向钾通道5抗体0.2ml/200μg产品应用 WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 Flow-Cyt=1:20-100 ICC=1:100-500 IF=1:50-200
(石蜡切片需做抗原修复)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
保存条件 Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
Important Note This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
产品介绍 Potassium channels are present in most mammalian cells, where they participate in a wide range of physiologic responses. The protein encoded by this gene is an integral membrane protein and inward-rectifier type potassium channel. The encoded protein, which has a greater tendency to allow potassium to flow into a cell rather than out of a cell, is controlled by G-proteins. It may associate with two other G-protein-activated potassium channels to form a heteromultimeric pore-forming complex. [provided by RefSeq, Jul 2008].
Function :
This potassium channel is controlled by G proteins. Inward rectifier potassium channels are characterized by a greater tendency to allow potassium to flow into the cell rather than out of it. Their voltage dependence is regulated by the concentration of extracellular potassium; as external potassium is raised, the voltage range of the channel opening shifts to more positive voltages. The inward rectification is mainly due to the blockage of outward current by internal magnesium. Can be blocked by external barium.
Subunit :
May associate with GIRK1 and GIRK2 to form a G-protein-activated heteromultimer pore-forming unit. The resulting inward current is much larger (By similarity).
Subcellular Location :
Membrane; Multi-pass membrane protein.
G蛋白激活内向钾通道5抗体0.2ml/200μg具有全、新、优、品、好四大特点:
全:公司提供上万种产品,涵盖了生物试剂,elisa试剂盒,标准品,培养基,原装耗材,抗体、培养基、ATCC细胞等,基本上各种科研所需产品在我司都能找到。
新:产品更新速度较快,基本上每周都有新产品出现。
优:产品质量好,投诉比较少。
好:我公司具有优质的技术团队,产品一旦售出,实验过程中遇到困难可提供在线技术咨询。使您使用产品时没有任何的后顾之忧。
G蛋白激活内向钾通道5抗体0.2ml/200μg实验步骤
① 滴加0.01mol/L,pH7.4的PBS于待检标本片上,10min后弃去,使标本保持一定湿度。
② 滴加适当稀释的荧光标记的抗体溶液,使其完全覆盖标本,置于有盖搪瓷盒内,保温一定时间(参考:30min)。
③ 取出玻片,置玻片架上,先用0.01mol/L,pH7.4的PBS冲洗后,再按顺序过0.01mol/L,pH7.4的PBS三缸浸泡,每缸3-5 min,不时振荡。
④ 取出玻片,用滤纸吸去多余水分,但不使标本干燥,加一滴缓冲甘油,以盖玻片覆盖。
⑤ 立即用荧光显微镜观察。观察标本的特异性荧光强度,一般可用“+”表示:
(-)无荧光;(±)极弱的可疑荧光;(+)荧光较弱,但清楚可见;(++)荧光明亮;(+++ --++++)荧光闪亮。待检标本特异性荧光染色强度达“++”以上,而各种对照显示为(±)或(-),即可判定为阳性。
G蛋白激活内向钾通道5抗体0.2ml/200μg现货供应,货期短,为您提供售前、售中、售后服务,保证实验结果,无效可以免费退换,提供免费代测服务,产品效价高、种类全、价格实惠 ,欢迎来电咨询!
CD163L1 CD163蛋白样1抗体 特价促销
CDAN1 先天性红细胞生成异常性贫血蛋白1抗体 特价促销
CDK2AP2 细胞周期素依赖激酶2相关蛋白2抗体 特价促销
CEACAM21 癌胚抗原相关细胞粘附分子21抗体 特价促销
CDKAL1 周期素依赖性激酶5调节蛋白1样蛋白1抗体 特价促销
CEACAM16/CEAL2 癌胚抗原相关细胞粘附分子16抗体 特价促销
CEACAM19/CEAL1 癌胚抗原相关细胞粘附分子19抗体 特价促销
ADCK5 ADCK5蛋白抗体 特价促销
ITGB1BP2/CHORDC3 整合素β1结合蛋白2抗体 特价促销
CLDND2 膜蛋白CLDND2抗体 特价促销
G蛋白激活内向钾通道5抗体0.2ml/200μgELISA Kit for Human Sodium/calcium exchanger 1 现货供应 0.156-10 ng/mL
ELISA Kit for Human Sodium-dependent serotonin transporter 现货供应 31.2-2000 pg/mL
ELISA Kit for Human Solute carrier family 12 member 1 现货供应 0.312-20 ng/mL
ELISA Kit for Human Solute carrier family 2, facilitated glucose transporter member 1 现货供应 0.156-10 ng/mL
ELISA Kit for Human Solute carrier family 2, facilitated glucose transporter member 3 现货供应 0.156-10 ng/mL
ELISA Kit for Human Solute carrier family 2, facilitated glucose transporter member 4 现货供应 0.156-10 ng/mL
ELISA Kit for Human Solute carrier family 40 member 1 现货供应 0.156-10 ng/mL
ELISA Kit for Human Somatoliberin 现货供应 0.39-25 ng/mL
ELISA Kit for Human Somatostatin 现货供应 6.25-400 pg/mL
ELISA Kit for Human Somatostatin receptor type 2 现货供应 78-5000 pg/mL
CD163L1 CD163蛋白样1抗体 特价促销
CDAN1 先天性红细胞生成异常性贫血蛋白1抗体 特价促销
CDK2AP2 细胞周期素依赖激酶2相关蛋白2抗体 特价促销
CEACAM21 癌胚抗原相关细胞粘附分子21抗体 特价促销
CDKAL1 周期素依赖性激酶5调节蛋白1样蛋白1抗体 特价促销
CEACAM16/CEAL2 癌胚抗原相关细胞粘附分子16抗体 特价促销
CEACAM19/CEAL1 癌胚抗原相关细胞粘附分子19抗体 特价促销
ADCK5 ADCK5蛋白抗体 特价促销
ITGB1BP2/CHORDC3 整合素β1结合蛋白2抗体 特价促销
CLDND2 膜蛋白CLDND2抗体 特价促销
注意事项
1)对荧光标记的抗体的稀释,要保证抗体的蛋白有一定的浓度,一般稀释度不应超过1:20,抗体浓度过低,会导致产生的荧光过弱,影响结果的观察。
2)染色的温度和时间需要根据各种不同的标本及抗原而变化,染色时间可以从10 min到数小时,一般30 min已足够。染色温度多采用室温(25℃左右),高于37℃可加强染色效果,但对不耐热的抗原(如流行性乙型脑炎病毒)可采用0-2℃的低温,延长染色时间。低温染色较37℃30 min效果好的多。
3)为了保证荧光染色的正确性,首次试验时需设置下述对照,以排除某些非特异性荧光染色的干扰。
① 标本自发荧光对照:标本加1-2滴0.01mol/L,pH7.4的PBS。
② 特异性对照(抑制试验):标本加未标记的特异性抗体,再加荧光标记的特异性抗体。
③ 阳性对照:已知的阳性标本加荧光标记的特异性抗体。
如果标本自发荧光对照和特异性对照呈无荧光或弱荧光,阳性对照和待检标本呈强荧光,则为特异性阳性染色。
4)一般标本在高压汞灯下照射超过3min,就有荧光减弱现象,经荧光染色的标本zui好在当天观察,随着时间的延长,荧光强度会
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G蛋白激活内向钾通道5抗体0.2ml/200μg
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