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- 文献和实验
- 技术资料
- 保存条件:
4°C
- 克隆性:
多克隆
- 抗体英文名:
Streptavidin/Biotin Blocking Kit
- 抗体名:
Streptavidin/Biotin Blocking Kit
- 规格:
1 kit
Description
Streptavidin/Biotin Blocking Kit blocks all endogenous biotin, biotin receptors, and streptavidin binding sites present in tissues. This kit is designed for use with streptavidin detection systems such as the VECTASTAIN® Universal Quick kits if streptavidin or biotinylated reagents bind non-specifically to tissues or proteins. This blocking kit consists of 18 ml Streptavidin and 18 ml of biotin in convenient dropper bottles.
http://vectorlabs.com/streptavidin-biotin-blocking-kit.html 欣博盛独家代理品牌vectorlabs,公司成立时,那时市面上还没有单克隆抗体、生物素-亲和素产品以及凝集素产品。1976, Vectorlabs率先纯化出凝集素产品,并迅速成为世界上该类产品的主要供应商。次年,Vetorlabs推出了生物素-亲和素系统。由于生物素和亲和素分子间极强的相互作用力,因此可以在生物素化的分子和结合有标记分子的亲和素间形成近似共价键的连接。Vectorlabs可提供抗原修复试剂、封闭试剂、复染试剂、亲和素/链霉亲和素-酶结合物、ABC免疫组化试剂盒、免疫组化显色底物、免疫组化抗体等。
详情请咨询Vectorlabs全国授权独,家代理商-欣博盛生物科技
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文献和实验Coordination between ECM and cell-cell adhesion regulates the development of islet aggregation, architecture, and functional maturation
Wilma Tixi, Maricela Maldonado, ..., Jonathan A Cooper
Article Snippet
"... 71676–01 , .Commercial assay, kit , AlphaTrak2 Meter , Zoetis , 71676–01 , .. Commercial assay, kit , Streptavidin/Biotin Blocking Kit , Vector Laboratories , SP-2002 , .. Commercial assay, kit , Rneasy Micro Kit , Qiagen , 74304 , .Commercial assay, ..." More
Figure Legend
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A High-Fat and High-Carbohydrate Diet Promotes Reminiscent Hallmarks of an Aging Ovary in the Rabbit Model
Verónica Díaz-Hernández, Luis M. Montaño, Célia M. Antunes
Article Snippet
"Subsequently, the slides were incubated with a blocking solution (10% horse serum in 0.5% triton/TBS (Cat. No. 97064-338NP, VWR Life Science, Missouri, TX, USA) for 2 h. Endogenous peroxidases and biotin were quenched with Bloxall (Cat. No. SP-6000, Vector Labs, Newark, CA, USA)) for 20 min and Streptavidin/Biotin Blocking Kit (Cat. No. SP2002, Vector Labs, Newark, CA, USA) for 15 min, respectively." More
Figure Legend
" Antibodies used for immunolocalization. " More
后将其移除。 纯化原理 Strep标签纯化蛋白质的技术原理,是利用自然界中最强的非共价作用之一:生物素(biotin)和链霉亲和素(streptavidin)之间的相互作用。将链霉亲和素改进后,就得到了非常稳定的Strep-Tactin®。Strep-Tactin®同样也是一种蛋白质,能高效且可逆的结合短肽标签Strep-tag®,而且它保留了结合生物素的能力。当生物素加入时,会作为特异性的竞争剂,用于洗脱Strep-tag®标签蛋白。 标签-配体 搭配Strep标签进行蛋白纯化的配体有2种
到 86%(B)(n = 2) 实验材料为什么使用 Strep-Tactin®TACS? 实验中使用的 Strep-Tactin®TACS 亲和层析柱,主要成分 Strep-Tactin® 是链霉亲和素(Streptavidin)的一种突变体,借助链霉亲和素-生物素作用原理,Strep-Tactin® 与生物素(biotin)的亲和力介于 100 pM – 100 fM 间。当向含有的 Strep-Tactin® 层析柱中加入生物素化的抗体时,便能将其固定在柱子中,特异性捕获细胞,进行阴选
A general protocol for staining cell for cytometry analysis
V-FITC (Cat. No. 65874X, 65874H) or Annexin V-Biotin (Cat. Nos. 65872X, 65872H). 3. Via-Probe™ (Cat. No. 34321X): A convenient ready-to-use solution of the nucleic acid dye 7-AAD. Recommended for use in parallel with Annexin V-PE (Cat
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