- ￥3523
- Vectorlabs
- 美国
- SA-5100
- 2026年02月27日
- Immunohistochemistry / Immunocytochemistry, In situ hybridization, Blotting Applications, Elispot, ELISAs
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- 详细信息
- 询价记录
- 文献和实验
- 技术资料
- 保存条件：
4°C
- 克隆性：
多克隆
- 级别：
科研用
- 库存：
少量现货
- 供应商：
欣博盛生物
- 应用范围：
Immunohistochemistry / Immunocytochemistry, In situ hybridization, Blotting Applications, Elispot, ELISAs
- 抗体英文名：
Alkaline Phosphatase Streptavidin
- 抗体名：
Alkaline Phosphatase Streptavidin
- 规格：
1 ml
Streptavidin, Alkaline Phosphatase产品货号SA-5100-1，规格是1 ml。如需购买Vectorlabs公司产品，请联系Veclabs公司授权总代理商欣博盛生物。
Alkaline Phosphatase Streptavidin (AP Streptavidin) is produced by our own coupling procedure that preserves the high specific activity of alkaline phosphatase. Using biotinylated primary or secondary intermediates and alkaline phosphatase-conjugated streptavidin, it is suitable for use in solid-phase assays, tissue/cell staining systems, and blotting applications.
Features:
• Avidin and streptavidin reagents are powerful tools to detect or purify biotinylated proteins, nucleic acids, and other macromolecules
• Vector Laboratories’ enzyme-conjugated avidin and streptavidin are produced with the highest specific activity enzymes in optimal ratios
• Specific covalent linkages are chosen to provide stable, highly active conjugates
Detection Enzyme
Alkaline Phosphatase
Format
Concentrate
Formulation
10 mM HEPES, pH 7.5, 0.15 M NaCl, 0.08% sodium azide, 1mM MgCl2, 1% sucrose, 2 mg/ml bovine serum albumin.
Unit Size
1 ml
Storage Instructions
2-8 °C
Usage Summary
The recommended dilution for use is 1:200 to 1:1000.(1-5 µg/ml). Storage of dilute solutions of the enzyme conjugate is not recommended.To avoid adsorption or denaturing of the enzyme conjugate, dilutions may be made in 10 mM HEPES, 0.15 M NaCl, pH 7.5, containing 0.1mg/ml immunohistochemical grade bovine serum albumin (Catalog No. SP-5050).DO NOT USE FRACTION V GRADE BSA.
Applications
Immunohistochemistry / Immunocytochemistry, In situ hybridization, Blotting Applications, Elispot, ELISAs
Conjugate
Alkaline Phosphatase
欣博盛独家代理品牌vectorlabs，公司成立时，那时市面上还没有单克隆抗体、生物素-亲和素产品以及凝集素产品。1976, Vectorlabs率先纯化出凝集素产品，并迅速成为世界上该类产品的主要供应商。次年，Vetorlabs推出了生物素-亲和素系统。由于生物素和亲和素分子间极强的相互作用力，因此可以在生物素化的分子和结合有标记分子的亲和素间形成近似共价键的连接。Vectorlabs可提供抗原修复试剂、封闭试剂、复染试剂、亲和素/链霉亲和素-酶结合物、ABC免疫组化试剂盒、免疫组化显色底物、免疫组化抗体等。
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文献和实验in solution c. for 5-15 minutes at room temperature. Wash, counterstain in haematoxylin, wash. Mount in phosphate buffered glycerine jelly RESULTS Alkaline phosphatase activity - Red Nuclei - Blue
Histochemical staining of sea urchin embryos for alkaline phosphatase (AP) enzyme activity
Histochemical staining of sea urchin embryos for alkaline phosphatase (AP) enzyme activity 1. Obtain embryo samples, tube of AP substrate buffer and tube of phosphate buffered saline ( PBS ) for each group. Allow embryos
1.1 General information Endothelial cells possess an endogenous alkaline phosphatase (AP) activity. The enzymatic activity of AP is not restricted to the endothelial lineage. In medaka embryos, somite cells during early developmental stages
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