- ￥3539
- Vectorlabs
- 美国
- H-1500
- 2026年02月27日
- Immunofluorescence, In situ hybridization, Cellular Imaging
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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
4°C
- 克隆性：
多克隆
- 保质期：
见包装说明
- 级别：
科研用
- 库存：
少量现货
- 供应商：
欣博盛生物
- 应用范围：
Immunofluorescence, In situ hybridization, Cellular Imaging
- 抗体英文名：
VECTASHIELD® HardSet™ with DAPI
- 抗体名：
VECTASHIELD® HardSet™ with DAPI
- 规格：
10 ml
VECTASHIELD HardSet Antifade Mounting Medium with DAPI is a unique, stable formula for preserving fluorescence. This medium prevents rapid photobleaching of fluorescent proteins and fluorescent dyes. VECTASHIELD HardSet Mounting Medium with DAPI hardens after coverslipping. After approximately 15 minutes at room temperature, the coverslip will become immobilized, and optimal antifade ability and refractive index will be achieved.
Features:
• Inhibits photobleaching of fluorescent dyes and fluorescent proteins
• Ideal refractive index (1.46)
• Ready-to-use – from the refrigerator to the benchtop
• No warming necessary
• Can be stored without sealing for long term analysis
• Hardening formulation
• DAPI counterstain
Antifade
Yes
Counterstain
DAPI (blue fluorescence)
Mounting Medium Type
Aqueous (Hardening)
Unit Size
10 ml
Applications
Immunofluorescence, In situ hybridization, Cellular Imaging
Cellular Stain
Nucleus
欣博盛独家代理品牌vectorlabs，公司成立时，那时市面上还没有单克隆抗体、生物素-亲和素产品以及凝集素产品。1976, Vectorlabs率先纯化出凝集素产品，并迅速成为世界上该类产品的主要供应商。次年，Vetorlabs推出了生物素-亲和素系统。由于生物素和亲和素分子间极强的相互作用力，因此可以在生物素化的分子和结合有标记分子的亲和素间形成近似共价键的连接。Vectorlabs可提供抗原修复试剂、封闭试剂、复染试剂、亲和素/链霉亲和素-酶结合物、ABC免疫组化试剂盒、免疫组化显色底物、免疫组化抗体等。
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文献和实验AAV2-mediated intravitreal delivery of exon-specific U1 snRNA rescues optic neuropathy in familial dysautonomia
Anil Chekuri, Krishnakanth Kondabolu, ..., Susan A. Slaugenhaupt
Molecular Therapy. Nucleic Acids | 2026 Mar 12 | Read Article | Bioz AI Brief
"cubated with secondary antibodies (Invitrogen; Jackson Immuno Research, West Grove, PA, USA) for 1 h at room temperature. Sections were mounted with hard-set mounting media containing DAPI (#H-1500-10 Vector Laboratories), and fluorescent microscopy was performed. One eye from each mouse was included in this analysis. Real-time quantitative polymerase chain reaction (RT-qPCR) was used to detect the" More...
Differential effects of the microbial metabolite acetate on murine microglia in in vitro sepsis and trauma models.
Qin Yang, Amanda Dave, ..., Dennis Simon
Brain research | 2026 Feb 15 | 41702482 | Read Article | Bioz AI Brief
"Bioz AI Summary In the study, VECTASHIELD Hardset Antifade Mounting Medium with DAPI from Vector Laboratories was used to mount and counterstain the nuclei of microglial cells, enabling accurate imagi…" More...
Vybrant® DyeCycle Green and Orange Stains
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性(图1)。NuPAGE® 样品缓冲液的pH值不会低于pH 8(即使是加热到70°C)，因此样品制备过程中蛋白样品不会降解。 图1. 看看NuPAGE® 凝胶的与众不同之处。(A) NuPAGE® 凝胶利用1×MES缓冲液运行。(B) tris-甘氨酸凝胶利用tris-甘氨酸/SDS缓冲液运行。图片显示，与tris-甘氨酸凝胶相比，NuPAGE® 凝胶产生锐利、完整的条带，代表了未水解的蛋白，特别是在第3、5、7、8和9道。 样品上样：(1) Mark12™
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