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文献和实验with the better the chances of a successful pulldown. A total of 4.0×106 293 HEK cells (~70% confluent) are plated and 24 hours later transfected with 15µg of a RNA Polymerase II expressing flag-tagged construct plasmid. We generally use Lipofectamine 2000™
Detection and Measurement of Radioactivity
. Methods of detection The method employed to detect radiation depends on the type of emitter and the intended purpose of detection. The most well known method of detecting radiation is with an ionization chamber. A high energy particle
Lumigen HyPerBlu provides sensitive chemiluminescent detection of hydrogen peroxide
it an excellent choice for examination of oxidase activity. The signal intensity is sufficient for measurement on PMT based plate reading luminometers or CCD imaging systems. HyPerBlu technology detection is suitable for automation and miniaturization to 384
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