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- 详细信息
- 文献和实验
- 技术资料
- 供应商:
欣博盛
- 英文名:
XCP 12, Chromosome 12 Whole Painting Probe
- 库存:
大量
- 保质期:
见说明书
- 规格:
200 µl
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文献和实验Preparation of Human Chromosomal Painting Probes From Somatic Cell Hybrids
Chromosomal in situ suppression (CISS) hybridization with flow-sorted chromosome libraries (FSCL) has provided a very powerful tool in human cytogenetics. This technique allows the painting of specific chromosomes in metaphase spreads
In situ hybridisation to alpha satellite sequences (chromosome specific)
hybridised to a chromosome 17 specific alpha satellite probe. We buy our probes from Appligene/Oncor although they are very expensive and the telesales staff are rude. Starting material We have two types of starting material; (i) tissue culture
In Situ Hybridization Using Digoxigenin Labeled Probes
bubbles 3) To prevent evaporation of the probe seal the edges of the coverslip with rubber cement 4) Incubate in a moist chamber at 58°C for 12-18 hours 5) Place the slide in a beaker containing 2X SSC and pry off the coverslip and rubber cement
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