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- 2025年08月04日
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- 详细信息
- 文献和实验
- 技术资料
- 保质期：
3years
- 供应商：
Neobioscience
- 保存条件：
-20°C
- 规格：
5ug
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文献和实验- Spin the cells on one for 5min and resuspend at a concentration of 1 x 106 cells/ml in media containing 10% DMSO. - Aliquot 1ml into cyrotubes and freeze in the -70oC freezer in a Mr Frosty overnight. Once frozen
containing 10ml of PMEF media. Incubate at 37oC for two days or until confluent IRRADIATING CELLS Once cells are confluent tighten the lid of the flask and take up to the gamma source in the animal house on the third floor (PMCI) Irradiate
FEEDER PREPARATION BY GAMMA IRRADIATION
ml tubes per every 50 ml centrifuge tube. 10. Cells are now ready to be gamma irradiated. You need to administer 6,000 rads. If you have not use the Irradiator, you need to ask Sandra, Sukeshi or Torrye. DO NOT USE THE IRRADIATOR
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