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- 详细信息
- 文献和实验
- 技术资料
- 抗体名:
Histone H3R8me2s Antibody
- 抗体英文名:
Histone H3R8me2s Antibody
- 靶点:
详见说明书
- 浓度:
1.0mg/ml
- 应用范围:
WB IF IP
- 宿主:
Human Mouse RatOtHumaneRat (Wide Ratange)
- 供应商:
上海钰博生物科技有限公司
- 库存:
大量
- 目录编号:
2527
- 抗原来源:
Human Mouse RatOtHumaneRat (Wide Ratange)
- 保质期:
1年
- 适应物种:
详见说明书
- 标记物:
详见说明书
- 克隆性:
多克隆
- 保存条件:
Store at -20°C
- 形态:
液体或冻干粉
- 亚型:
详见说明书
- 免疫原:
详见说明书
- 规格:
50μg/50μl, 100μg/100μl
-
- Catalog No. : Yb--2527
- Size : 50μg/50μl, 100μg/100μl
-
- Mol Weight : 15kDa
- UniProt : Q16695
-
- Isotype : Rabbit IgG
- Clonality : Polyclonal
-
- Price : $265/100μg
- Availability : Ship next day
-
Category
- Primary Antibodies
- Secondary antibody
- Loading control / tag antibody
Details
Alternative Names:
HIST1H3J; H3/j ;H3FJ ; Histone H3.1; Histone H3/a; Histone H3/b;Histone H3/c; Histone H3/d; Histone H3/f;Histone H3/h ; Histone H3/I;HistoneH3/j ;Histone H3/k; Histone H3/l;HIST3H3;
Application:
WB IF IP
Reactivity:
Human Mouse RatOtHumaneRat (Wide Ratange)
Dilution:
WB 1:500-1:2000 IF 1:50–1:200 IP 1:50–1:200
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Western blot analysis of extracts of HeLa cellline and H3 protein expressed in E.coli., usingH3R8me2s antibody|Dot-blot analysis of all sorts of methylation peptidesusingH3R8me2s antibody|Immunofluorescence analysis of 293T cell usingH3R8me2s antibody. Bl |
Purification:
Affinity-chromatography
Specificity:
Histone H3R8me2s Antibody detects endogenous levels of total Histone H3R8me2s
Immunogen:
A synthesized peptide derived from human Histone H3R8me2s
Description:
Storage Condition and Buffer:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.
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文献和实验in detail here. The first protocol is essentially a dual-pulldown assay employing Flag-tagged DNMT3A or antibody of choice for an endogenous protein and 5' biotin linked antisense RNA (Figure 1a), while the second protocol is a triple pulldown assay which essentially expands
9.19 Nature:揭示组蛋白标记 H3K36me2 招募 DNMT3A 并影响基因间 DNA 甲基化
中心、洛克菲勒大学和加拿大麦吉尔大学等研究机构的研究人员报道 NSD1 介导的 H3K36me2 是在基因间区域招募 DNMT3A 和维持 DNA 甲基化所必需的。这项新的研究揭示了一种跨染色质调节途径,这种调节突进将异常的基因间 CpG 甲基化与人类肿瘤生长和过度发育相关联在一起。原文检索:The histone mark H3K36me2 recruits DNMT3A and shapes the intergenic DNA methylation landscape ② Nature 突破
PROTOCOL To 1.5 mL eppendorf tubes add: 200 µg of protein extract (see Western blot protocol for protein sample preps) q.s. to 300 µL with RIPA (with protease and phosphatase inhibitors). Primary antibody (amount determined
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