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- 详细信息
- 文献和实验
- 技术资料
- 保修期:
1年
- 现货状态:
现货
- 供应商:
IKA
- 规格:
0003958025
IKA® mini离心机适用于所有不需要高速运作的应用领域, 如微过滤和细胞分离。透明的顶盖可以确保用户能随时明了地对实验过程进行察看。IKA® mini离心机还能与PCR条管配套使用。只有在顶盖关闭的情况Mini离心机才会运行,增强了用户的使用安全性。轻轻一按开启按钮顶盖即可打开。
- 恒定转速 6000rpm
- 可调定时范围(1-99 分钟)
- 智能快速停机功能
- 快速启动
- 精巧设计,静音运行
- 转子更换无需任何辅助工具
- 触摸式按键操作简便
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文献和实验A quick RNA mini-prep for Neurospora mycelial cultures
, during time series analyses or when screening transfor-mants for expression of a transformed gene. Under such circumstances, existing tech-niques are overly time consuming and yield more RNA than is necessary. The availability of a rapid RNA mini-prep
is disrupted by Òalkali-lysisÓ to release DNA which is then separated from the rest of the cellular components. Traditional methods have included the use of organic solvent extractions (e.g. phenol extraction was used for mini-preps) or ultracentrifugation (e.g
Fast and reliable mini-prep RNA extraction from Neurospora crassa
, mycelia from plates (4 days old) and single colonies of N. crassa grown on a sorbose plate. With this protocol, from 20 to 300 mg (dry weight) of mycelium, grown in liquid media or on plates, yielded between 200 µg and 3 mg of RNA depending on the growth
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