- ¥2900
- ACRO
- CD9-HF251
- 2025年09月03日
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- 详细信息
- 询价记录
- 文献和实验
- 技术资料
- 英文名：
FITC-Labeled Human CD19, Fc Tag蛋白
- 供应商：
研卉生物
- 规格：
25ug
1. FITC-Labeled Human CD19, Fc Tag蛋白（Cat. No. CD9-HF251 ）
> 适用于Anti-CD19-CAR阳性表达率检测
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- 图5. Binding activity of FITC-Labeled Human CD19, His Tag from two different vendors were evaluated in the above ELISA analysis against FMC63 MAb. The result showed that ACRO's FITC-Labeled Human CD19, His Tag has a much higher binding activity than Other Vendor's.
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- 图6. Binding activity of FITC-Labeled Human CD19, His Tag from two different vendors were evaluated in the above flow cytometry analysis against anti-CD19-CAR-293 cells. The result showed that ACRO's FITC-Labeled Human CD19, His Tag has a much higher binding activity than Other Vendor's.
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- 图7. Binding activity of Human CD19, Fc Tag from two different vendors were evaluated in the above ELISA analysis against FMC63 MAb. The result showed that ACRO's Human CD19, Fc Tag has a higher binding activity than Other Vendor's.
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- 图8. Binding activity of Human CD19, Fc Tag from two different vendors were evaluated in the above flow cytometry analysis against anti-CD19-CAR-293 cells. The result showed that ACRO's Human CD19, Fc Tag has a higher binding activity than Other Vendor's.
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- 图10. The purity of Human CD19, Fc Tag from two different vendors were determined in HPLC analysis. The result showed that the purity of ACRO’s Human CD19, Fc Tag is greater than 87%, while the purity of Other Vendor’s Human CD19, Fc Tag is greater than 57%.
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- 图11. Binding activity of Human CD19, His Tag from two different vendors were evaluated in the above ELISA analysis against FMC63 MAb. The result showed that ACRO's Human CD19, His Tag has a much higher binding activity than Other Vendor's.
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- 图12. Binding activity of Human CD19, His Tag from two different vendors were evaluated in the above flow cytometry analysis against anti-CD19-CAR-293 cells. The result showed that ACRO's Human CD19, His Tag has a much higher binding activity than Other Vendor's.
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- 图13. Binding activity of Human CD19, His Tag from two different vendors were evaluated by SPR assay against FMC63 MAb. The result showed that ACRO's Human CD19, His Tag can bind FMC63 MAb with an affinity constant of 2.95 nM which is much higher than Other Vendor's Human CD19, His Tag.
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文献和实验CAR-T 攻克实体瘤又出新招！超声「遥控」细胞靶向治疗癌症
的 B 细胞表面也表达 CD19 抗原，导致 CAR-T 细胞在杀伤肿瘤细胞的同时也杀伤了正常细胞。因此对 CAR-T 细胞进行高精度控制，使其杀伤癌细胞的同时减少对正常细胞的伤害是 CAR-T 疗法改进的重要方向。超声波可以将机械能安全、无创地输送到身体表面数十厘米以下的部位。聚焦超声波（FUS）的快速振荡压力以及由此产生的机械能可使生物组织的局部发热。在磁共振成像（MRI）测温技术的帮助下，FUS 已在临床上被广泛应用于消融肿瘤和控制药物释放、血管舒张、神经调节和转基因表达等方面。然而，目前尚缺乏利用
批次的未耗竭CAR-T细胞进行比较。每天从检测板的所有孔中提取细胞因子样品（10 μL），72小时后，使用iQue®人类T细胞耗竭试剂盒对细胞和上清液进行量化。结果如图3所示。 图3. CD19+ Ramos细胞系激发抗-CD19的CAR-T细胞表现出明显的耗竭表型 细胞因子的分泌，包括IFNγ和TNFα，在含有耗竭的CAR-T和Ramos细胞共培养的孔中均很低（图3A和B）。相对而言，新鲜CAR-T细胞分泌的IFNγ和TNFα水平明显较高，48小时的峰值浓度分别为3.5±0.2 ng
捕获平台：烈冰生物 10X Genomics 平台 生信数据分析平台：NovelBrain® 生信分析系统 数据分析工具：细胞聚类分析，Marker gene 鉴定，GSEA 分析，Qusage 分析等 3.实验设计 （1）非病毒定点整合 CAR-T 细胞的制备 首先，为提高大片段 CAR 序列在 T 细胞中的整合效率，团队通过大量实验摸索和优化，发现以同源臂长度为 800bp 的线性双链 DNA 作为模板，可获得最好的 CAR-T 整合效果，并利用 AAVS1 位点进一步制备了靶向 CD19 的定点
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