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- 详细信息
- 文献和实验
- 技术资料
- 提供商:
上海康朗生物科技有限公司
- 规格:
50ug
外泌体(Exosome)是直径约为30-150nm,密度在1.13-1.21g/m1的小囊泡。Exosome天然存在于体液中,包括血液、唾液、尿液和母乳,外泌体是活细胞分泌的来源于晚期核内体(也称为多囊泡体 )的膜性囊泡。
Anti-human CD9 Antibody Biotin Conjugated研究发现,Exosome内含有与细胞来源相关的蛋白质rRNA和microRNA,并且exosome能够通过生物屏障,在细胞间传递功能性核酸分子,从而发挥各种生物学功能。
Anti-human CD9 Antibody Biotin Conjugated服务内容:
1,外泌体提取服务
从体外培养的细胞中分离和提取exosome的主要方法是超速离心法,这种方法缺点也不少:耗时耗力,往往需要30个小时左右;每次最多只能处理6个样品;需要大量的起始材料;产量不高。
我公司自主开发外泌体快提取试剂盒,快速高效获得高纯度外泌体颗粒。
- 外泌体电镜检测服务
全血,血浆;
细胞上清液;
体液:尿液、心包积液、唾液、脑脊液、腹水等。
用于分离外泌体类型及所需量:
血清样本: 5ml (全血10ml);
血浆样本: 5ml (全血 8-10ml);
无血清培养细胞上清:50ml;
体液: 20ml;
Anti-human CD9 Antibody Biotin Conjugated外泌体粒径检测服务
来自细胞培养上清液提取的外泌体,通过美国PSS Nicomp 380 Z3000粒度仪检测
3,外泌体基因、蛋白检测分析服务
外泌体microRNA检测分析;
外泌体lncRNA检测分析;
外泌体蛋白表达检测分析;
4,外泌体NSG测序服务
Anti-human CD9 Antibody Biotin Conjugated相关产品
外泌体提取纯化试剂盒;
Cd63-GFP真核表达质粒;
Cd63-GFP腺病毒颗粒;
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文献和实验Isolation of Anti-Hapten Specific Antibody Fragments from Combinatorial Libraries
system unless presented as an epitope conjugated to a suitable immunogenic carrier protein, such as bovine thyroglobulin. The principal interest in anti-hapten Abs is as detection molecules for use in diagnostic assays. These typically use dipstick (qualitative
The Avidin-Biotin Complex (ABC) Method and Other Avidin-Biotin Binding Methods
for each species of primary antibody used. The biotin molecule is small and can be easily conjugated to immunoglobulin by amino substitution at alkaline pH without the loss of immunoglobulin activity. These secondary antibodies are quite inexpensive, readily
The Avidin-Biotin Complex(ABC) Method
, between the horseradish peroxidase enzyme and a secondary antibody reagent, enzyme localization can be achieved at the site of primary antibody interaction with the specimen (see ) Fig. 1 ). The biotin molecule is small and can be easily conjugated to immunoglobulin
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