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- Abbiotec
- 美国
- 350479
- 2025年07月09日
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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
Store at -20°C. The product is hygroscopic and must be protected from light. Product is guaranteed one year from the date of shipment. Following reconstitution, store at -20°C.
- 保质期：
参照说明书可保存1-2年
- 英文名：
PERK Peptide
- 库存：
一定量库存
- 供应商：
华拓生物
- 规格：
0.1 mg
| 产品名称： | PERK Peptide |
| 产品链接： | http://www.abbiotec.com/peptides/perk-peptide |
| 货号： | 350479 |
| 规格： | 0.1 mg |
| 产品描述： | PERK, a Ser/Thr protein kinase, phosphorylates the alpha subunit of eukaryotic translation-initiation factor 2 (EIF2), leading to its inactivation and to a rapid reduction of translational initiation and repression of global protein synthesis. PERK serves as a critical effector of unfolded protein response (UPR)-induced G1 growth arrest due to the loss of cyclin D1. Defects in PERK are the cause of Wolcott-Rallison syndrome, an early-onset diabetes mellitus. |
| 序列： | The synthetic peptide used to raise the antibody Cat. No. 200117 is selected from a sequence within the C-term region of human PERK. For blocking experiments, a 10 to 100 fold molar excess to antibody is recommended. |
| 分子式： | C79H141N35O28S1 |
| 分子量： | 2061.2 g/mol |
| 纯度： | Purity > 80% by HPLC |
| 别名： | Eukaryotic translation initiation factor 2-alpha kinase 3; PRKR-like endoplasmic reticulum kinase; Pancreatic eIF2-alpha kinase; HsPEK; EIF2AK3; PEK; PERK |
| 形态： | Each vial contains 0.1 mg of lyophilized peptide. Reconstitute with 0.1 ml deionized water for a final concentration of 1 mg/ml. Use at 5.6 ug/ml for a 100X excess over antibody for maximum blocking effect. |
| 溶解方法： | Distilled water for a solution up to 2 mg/ml, otherwise we recommend using acetonitrile. |
| 保存方法： | Store at -20°C. The product is hygroscopic and must be protected from light. Product is guaranteed one year from the date of shipment. Following reconstitution, store at -20°C. |
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文献和实验wuxin_1999 很疑惑的一个现象，我在检测细胞内pErk的水平，但是western做出来的条带怎么有时候是两条带有时候是一条带？pErk1/2的表达和细胞培养条件有怎样的关系呢？哪位给些建议啊 littleniu 一般ERK1的条带比ERK2要弱一点，所以如果你样品没做好或者胶没跑好，上面一条带就可能没有的，只要细胞状态好，ERK表达都很强的，选好你细胞所需的培养条件就可以。 shqling
二个以上的α -氨基酸以肽键连接起来而形成的化合物。根据构成氨基酸的数目 2、 3、 4……叫做二肽（ dipeptide）、三肽、四肽……。大体上由 10以下的肽键形成的肽总称为寡肽 （ Oligopeptide），由多数肽键构成的肽总称为多肽。每个肽按照构成它的氨基酸的名称，从具有游离α -氨基的末端氨基酸依次命名。例如 称为甘氨酰丙氨酸的二肽。蛋白质是由一条或几条长链状多肽构成的。象谷胱甘肽和肌肽那样的低
Two dimensional peptide mapping
This specfic protocol is the latest incarnation of peptide mapping procedures that have been developed here in the TVL/MBVL of the Salk Institute over the last 20 years. Wade Gibson developed the first peptide mapping protocol
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