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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
Store at -20°C. The product is hygroscopic and must be protected from light. Product is guaranteed one year from the date of shipment. Following reconstitution, aliquot and store at -20°C.
- 保质期:
参照说明书可保存1-2年
- 英文名:
DYKDDDDK Peptide
- 库存:
一定量库存
- 供应商:
华拓生物
- 规格:
1 mg
| 产品名称: | DYKDDDDK Peptide |
| 产品链接: | http://www.abbiotec.com/peptides/dykddddk-peptide |
| 货号: | 350178 |
| 规格: | 1 mg |
| 产品描述: | The DYKDDDDK peptide (Flag) is used as a fusion tag for immunoaffinity chromatography. The monoclonal antibody M1 binds the peptide in presence of bivalent metal cations such as Ca2+. Flag is a registered trademark of Sigma-Aldrich. |
| 序列: | H-Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys-OH or H-DYKDDDDK-OH |
| 分子式: | C41H60N10O20 |
| 分子量: | 1012.99 g/mol |
| 纯度: | Purity > 95% by HPLC |
| 别名: | DYKDDDDK; DYKDDDDK Tag; FLAG; FLAG Tag; FLAG DYKDDDDK; DDK; ECS tag; Enterokinase cleavage site epitope tag; Enterokinase cleavage site |
| 形态: | Each vial contains 1 mg of lyophilized solid packaged under an inert gas and supplied as a trifluoroacetate salt. |
| 溶解方法: | Distilled water for a solution up to 2 mg/ml, otherwise we recommend using acetonitrile. |
| 保存方法: | Store at -20°C. The product is hygroscopic and must be protected from light. Product is guaranteed one year from the date of shipment. Following reconstitution, aliquot and store at -20°C. |
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文献和实验Anti-DYKDDDDK tag (L5) Affinity Gel
DYKDDDDK peptide to compete the binding to the L5 resin. 4. Run a SDS-gel for all the elute aliquots and combine the ones with desired concentration and purity base on the gel result. Dialyze the protein product into appropriate buffer and stored
二个以上的α -氨基酸以肽键连接起来而形成的化合物。根据构成氨基酸的数目 2、 3、 4……叫做二肽( dipeptide)、三肽、四肽……。大体上由 10以下的肽键形成的肽总称为寡肽 ( Oligopeptide),由多数肽键构成的肽总称为多肽。每个肽按照构成它的氨基酸的名称,从具有游离α -氨基的末端氨基酸依次命名。例如 称为甘氨酰丙氨酸的二肽。蛋白质是由一条或几条长链状多肽构成的。象谷胱甘肽和肌肽那样的低
Two dimensional peptide mapping
This specfic protocol is the latest incarnation of peptide mapping procedures that have been developed here in the TVL/MBVL of the Salk Institute over the last 20 years. Wade Gibson developed the first peptide mapping protocol
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