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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
Store at -20°C. The product is hygroscopic and must be protected from light. Product is guaranteed one year from the date of shipment. Following reconstitution, aliquot and store at -20°C.
- 保质期:
参照说明书可保存1-2年
- 英文名:
CEF21 Peptide
- 库存:
一定量库存
- 供应商:
华拓生物
- 规格:
2 mg
| 产品名称: | CEF21 Peptide |
| 产品链接: | http://www.abbiotec.com/peptides/cef21-peptide |
| 货号: | 350121 |
| 规格: | 2 mg |
| 产品描述: | CEF21 is a peptide from the human cytomegalovirus (CMV, human herpesvirus 5) PP65 protein that is used for pulsing antigen-presenting cells. |
| 序列: | CMV: H-Thr-Pro-Arg-Val-Thr-Gly-Gly-Gly-Ala-Met-OH or H-TPRVTGGGAM-OH |
| 分子式: | C38H67N13O13S1 |
| 分子量: | 946.1 g/mol |
| 纯度: | Purity > 95% by HPLC |
| 别名: | 65 kDa lower matrix phosphoprotein; PP65; tegument protein UL83 |
| 形态: | Each vial contains 1 mg of lyophilized solid packaged under an inert gas and supplied as a trifluoroacetate salt. |
| 溶解方法: | Distilled water for a solution up to 2 mg/ml, otherwise we recommend using acetonitrile. |
| 保存方法: | Store at -20°C. The product is hygroscopic and must be protected from light. Product is guaranteed one year from the date of shipment. Following reconstitution, aliquot and store at -20°C. |
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文献和实验1.纯化病毒第一步剪碎法CEF的制备实验前准备PH7.2 PBS(SW配)﹑平皿一小包(3个)﹑饭盒1(器械,玻璃漏斗)﹑饭盒2(纱布)﹑离心管、碘酊﹑废液缸﹑0.1%新洁尔灭溶液﹑0.25%胰酶﹑9日龄SPF胚2﹑M199培养基实验步骤1)首先SPF胚新洁尔灭溶液表面消毒,再碘酊﹑酒精消毒,最后小心敲破气室2)准备两把眼科弯头镊子,一把撕破尿囊膜和羊膜,另一把夹鸡胚。用镊子把鸡胚取出,浸泡于已倒入PBS的平皿中①去爪②去内脏3)把鸡胚从平皿中取出,置于另一含PBS的平皿中,再一次洗涤4)重复
二个以上的α -氨基酸以肽键连接起来而形成的化合物。根据构成氨基酸的数目 2、 3、 4……叫做二肽( dipeptide)、三肽、四肽……。大体上由 10以下的肽键形成的肽总称为寡肽 ( Oligopeptide),由多数肽键构成的肽总称为多肽。每个肽按照构成它的氨基酸的名称,从具有游离α -氨基的末端氨基酸依次命名。例如 称为甘氨酰丙氨酸的二肽。蛋白质是由一条或几条长链状多肽构成的。象谷胱甘肽和肌肽那样的低
Two dimensional peptide mapping
This specfic protocol is the latest incarnation of peptide mapping procedures that have been developed here in the TVL/MBVL of the Salk Institute over the last 20 years. Wade Gibson developed the first peptide mapping protocol
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