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文献和实验Bacteriophage Enrichment from Water and Soil
Classical bacterial enrichment devised by Sergius Winogradsky (1856–1953) and Martinus Beijerinck (1851–1931) can be modified to enrich for bacteria-specific viruses. In this chapter simple protocols are presented for the enrichment of phages
it with phenol/chloroform, and precipitating it with ethanol. The DNA is resuspended at a concentration of 10 mg/ml in TE, placed in a boiling water bath for 20 min., and immediately cooled on ice. The carrier DNA solution can be stored in aliquots at-20°C.
and denatures the proteins allowing more reproducible results.SOLUTIONS:2% Na2CO3,0.02% NaK tatrate,0.1M NaOH,1% SDS (added last).0.5% CuSO4.On the day of the assay mix 25ml of solution A with 1ml of solution B.Dilute Folin reagent to 1N with deionized water
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