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文献和实验.EukaryotesBy contrast to the simple broth cultures of E. coli, the nutrient requirements of even the simplest eukaryotic culture are complex. Refer to Table 12.1 for a comparison of the ingredients of Nutrient Broth and Minimum Essential Media (MEM), a typical
Preparation of Genomic DNA from Bacteria- using Phase Lock GelTM
Preparation of Genomic DNA from Bacteria - using Phase Lock GelTM (Modified from Experimental Techniques in Bacterial Genetics, Jones and Bartlet, 1990)
. The method consists of an enrichment step in modified tryptone soya broth, followed by immunomagnetic separation and extraction of deoxyribonucleic acid. Real-time polymearse chain reaction, using hybridization probes, is used to detect the verotoxin genes
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