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文献和实验Human Peripheral Blood Mononuclear Cell Preparation
and institutional policies for proper handling instructions for human blood.2. The bubbles trap red blood cells.3. To deplete monocytes, culture the mononuclear cell mixture in several 10 cm dishes for 30 minutes to 1 hour. Monocytes adhere to the plastic dish
Human Peripheral Blood Mononuclear Cell Preparation
from the final cell sample. The isolated cells can be used in chemotaxis assays Procedure Hint #1 ) from the bag into a 125 ml conical polypropylene centrifuge tube and dilute to 90 ml with sterile endotoxin-free PBS. 2. Add 15 ml of sterile
-hot polyclonal, or 1:30 dilution of tissue culture supernatant for a good monoclonal. Do the incubation in a sealameal bag for 1 hr at room temp on a rocker. Can go overnight in the fridge. Remember: it's critical to do a control with no 1° antibody
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