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文献和实验Clonality - X Chromosome Inactivation Assay
of the resuspended DNA into a reaction tube. Add 1 µl REact buffer 2. Add 1 µl Hha I. Incubate overnight at 37°C. Incubate at 90°C for 10 min. Use 2 µl of this digested DNA for PCR (see below). Use 2 µl of the non-digested DNA from B18 above as a negative
ChIP protocol for X. laevis Lens1/FoxE3 enhancer
with 0.5x MBS at RT. (5) Transfer tissues into a 1.5 ml microfuge tube using a wide-bore plastic pipette. Remove the MBS as much as possible. (6) You may store the tissues at �80°C (use liquid nitrogen to freeze them). (7) Add 0.5 ml of �
RNA Purification Protocol for 1-2 x 107 cultured cells
设备 1. Microcentrifuge capable of 3,000 x g 2. 15 ml centrifuge tube 3. Ice bath 4. Ground pestle 实验步骤 1. Remove the media and collect cells. For cells grown in suspension, pellet the cell
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