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- Cellgro
- 30-003-CF
- 2025年09月16日
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999
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6 x 50ml
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文献和实验A general protocol for staining cell for cytometry analysis
6 cells/ml. 2. Transfer 100 µl of the solution (~1 x 10 5 cells) to a 5 ml culture tube. 3. Add 5-15 µg of purified recombinant Annexin V. The amount of purified recombinant Annexin V required to saturate binding sites may vary
lysis buffer (high salt) ) 2 x 1 ml CHIP wash buffer 2 x 1 ml TE elute immunoprecipitations: add 75 µl elution buffer incubate for 10 min at 65 °C spin, take supernatant, elute pellet again with 75 µl
A general protocol for staining cell for cytometry analysis
General Annexin V Staining Procedure Solutions 1.10X Binding Buffer (Cat. No. 66121A):0.1 M HEPES, pH 7.4; 1.4 M NaCl; 25 mM CaCl 2 . Dilute to 1× prior to use. 2. Propidium Iodide (PI). Prepare a 50 µg/ml stock solution of PI in 1× PBS buffer
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