Western blot detection of JNK1 in C6 and 3T3 cell lysates using JNK1 mouse mAb (1:1000 diluted).Predicted band size:46,54KDa.Observed band size:46KDa.
Immunofluorescent analysis of 3T3 cells fixed by anhydrous methanol for 2 h at -20℃ and using anti-JNK1 mouse mAb (dilution 1:100).
Purification:
Affinity-chromatography
Specificity:
JNK1 Mouse mAb detects endogenous levels of total JNK1
Immunogen:
Purified recombinant human JNK1 protein fragments expressed in E.coli.
Description:
Serine/threonine-protein kinase involved in various processes such as cell proliferation, differentiation, migration, transformation and programmed cell death. Extracellular stimuli such as proinflammatory cytokines or physical stress stimulate the stress-activated protein kinase/c-Jun N-terminal kinase (SAP/JNK) signaling pathway.
Storage Condition and Buffer:
Mouse IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.