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- 详细信息
- 文献和实验
- 技术资料
- 抗体名:
Akt(pan) Mouse mAb
- 抗体英文名:
Akt(pan) Mouse mAb
- 靶点:
详见说明书
- 浓度:
1.0mg/ml
- 应用范围:
WB
- 宿主:
Mouse
- 供应商:
上海钰博生物科技有限公司
- 库存:
大量
- 目录编号:
3146
- 抗原来源:
Mouse
- 保质期:
1年
- 适应物种:
详见说明书
- 标记物:
详见说明书
- 克隆性:
多克隆
- 保存条件:
Store at -20°C
- 形态:
液体或冻干粉
- 亚型:
详见说明书
- 免疫原:
详见说明书
- 规格:
50μg/50μl, 100μg/100μl
-
- Catalog No. : Yb--3146
- Size : 50μg/50μl, 100μg/100μl
-
- Mol Weight : 60KDa
- UniProt : P31749;P31751;Q9Y243
-
- Isotype : Mouse IgG2b
- Clonality : Monoclonal
-
- Price : $265/100μg
- Availability : 3-4 days
-
Category
- Primary Antibodies
- Secondary antibody
- Loading control / tag antibody
Details
Alternative Names:
PKB;RAC;PRKBA;PKB-ALPHA;RAC-ALPHA; AKT1 ; AKT2; AKT3;
Application:
WB
Reactivity:
Mouse,Rat
Dilution:
WB 1:1000
![]() |
Western blot detection of total AKT in rat heart,rat testis,mouse brain,mouse spleen and C6 cell lysates and using AKT(pan) mouse mAb (1:1000 diluted).Predicted band size: 60KDa.Observed band size: 60KDa. |
Purification:
Affinity-chromatography
Specificity:
Akt(pan) Mouse mAb detects endogenous levels of total Akt(pan)
Immunogen:
Purified recombinant human AKT1 protein fragments expressed in E.coli.
Description:
AKT1 is one of 3 closely related serine/threonine-protein kinases (AKT1, AKT2 and AKT3) called the AKT kinase, and which regulate many processes including metabolism, proliferation, cell survival, growth and angiogenesis. This is mediated through serine and/or threonine phosphorylation of a range of downstream substrates. Over 100 substrate candidates have been reported so far, but for most of them, no isoform specificity has been reported.
Storage Condition and Buffer:
Mouse IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.
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文献和实验米宝 这俩天做实验,极其郁闷。我做的总AKt和pAkt,大鼠脑组织,第一次跑总AKT的时候,我觉得可能是因为蛋白是提取的浓度比较好吧,出来了一条浓浓的条带,三组总AKt都出了,接下来做pAkt,就出了一个带,效果挺明显的。后来再做pAkt的时候,跑了4张膜,显影的时候,太干净了,上面什么都没有,甚至连个杂带,斑点 都没!实验是在实验员的领导下完成的,技术上基本没什么纰漏,查阅本版面有关这问题方面的介绍,有的解释说pakt降解了!一抗是用的Phospho-Akt
(adjust pH to 7.8 with Binding buffer; red color) to the Protein A column.Mouse antibodies of the IgG1 subclass do not have a high affinity for protein A. Purification on protein A beads using standard conditions will yield approximately 1/10
组织化学分析。(图 C)使用Anti-Erk1/2 Mouse mAb 鼠单抗进行目的蛋白检测;(图 D) 使用p44/42 MAPK (Erk1/2)Rabbit mAb 兔单抗进行目的蛋白检测。 图 7 免疫组化实验检测Akt表达 注:使用 Anti-Akt (pan) Rabbit mAb 对正常小鼠肝组织进行免疫组织化学分析。(图 E)使用免疫组化试剂盒 M&R HRP/DAB Detection IHC Kit,抗体1:100 稀释;(图F)使用另一种试剂盒,抗体 1:100 稀释。
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