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50ul/100ul/200ul
| 规格: | 50ul | 产品价格: | ¥1180.0 |
|---|---|---|---|
| 规格: | 100ul | 产品价格: | ¥1980.0 |
| 规格: | 200ul | 产品价格: | ¥2800.0 |
| 产品编号 | bs-23473R |
| 英文名称 | 58K Golgi protein Rabbit pAb |
| 中文名称 | 58K高尔基蛋白抗体 |
| 英文别名 | Formimidoyltetrahydrofolate cyclodeaminase; Formimidoyltransferase cyclodeaminase; Formiminotetrahydrofolate cyclodeaminase; Formiminotransferase cyclodeaminase; Formiminotransferase-cyclodeaminase; FTCD; FTCD_HUMAN; Glutamate formiminotransferase; Glutamate formyltransferase; LCHC 1; LCHC1. |
| 产品应用 | WB=1:500-2000 Not yet tested in other applications. |
| 交叉反应 | Mouse, Rat (Human, Pig, Cow, Sheep) |
| 抗体来源 | Rabbit |
| 免疫原 | KLH conjugated synthetic peptide derived from human 58K Golgi protein |
| 亚型 | IgG |
| 性状 | Liquid |
| 纯化方法 | affinity purified by Protein A |
| 克隆类型 | Polyclonal |
| 理论分子量 | 59 kDa |
| 浓度 | 1mg/ml |
| 储存液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 研究领域 | Metabolism > Pathways and Processes > Metabolic signaling pathways > Amino acid metabolism Metabolism > Types of disease > Cancer Signal Transduction > Metabolism > Amino Acids Tags & Cell Markers > Subcellular Markers > Organelles > Golgi |
| 亚细胞定位 | Cytoplasm, cytoskeleton, centrosome, centriole. Golgi apparatus. More abundantly located around the mother centriole. |
| 相似性 | In the C-terminal section; belongs to the cyclodeaminase/cyclohydrolase family.In the C-terminal section; belongs to the cyclodeaminase/cyclohydrolase family. In the N-terminal section; belongs to the formiminotransferase family. In the N-terminal section; belongs to the formiminotransferase family. |
| 功能 | Folate-dependent enzyme, that displays both transferase and deaminase activity. Serves to channel one-carbon units from formiminoglutamate to the folate pool. Binds and promotes bundling of vimentin filaments originating from the Golgi. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 背景资料 | The protein encoded by this gene is a bifunctional enzyme that channels 1-carbon units from formiminoglutamate, a metabolite of the histidine degradation pathway, to the folate pool. Mutations in this gene are associated with glutamate formiminotransferase deficiency. Alternatively spliced transcript variants have been found for this gene.[provided by RefSeq, Dec 2009] |
| 应用 | 推荐稀释比例 |
| {WB} | {1:500-2000} |

Liver(Mouse) Lysate at 40 ug
Liver(Rat) Lysate at 40 ug
Primary: Anti-58K Golgi protein (bs-23473R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 59 kD
Observed band size: 59 kD
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文献和实验Immunity:北大蒋争凡课题组发现 STING 后高尔基体囊泡转运的重要性及机制
: https://doi.org/10.1016/j.immuni.2023.02.004 参考文献: 1 Fang, R. et al. Recent advances in the activation and regulation of the cGAS-STING pathway. Advances in Immunology 156, 55-102, doi:10.1016 /bs.ai.2022.09.003 (2022). 2 Posor, Y. et al.
Nucleic Acid Programmable Protein Arrays: Versatile Tools for Array‐Based Functional Protein Studies
scale. Methods Mol. Biol. 375:57‐78. Li, Q.Z., Zhou, J., Wandstrat, A.E., Carr‐Johnson, F., Branch, V., Karp, D.R., Mohan, C., Wakeland, E.K., and Olsen, N.J. 2007. Protein
= NAL), appears to form upon oxidative cyclization of the nonfluorescent 2:1 lysine-HNE Michael adduct-Schiff base cross-link (Scheme 1). Polyclonal antibody (PAb) to the NAL-HNE fluorophore was raised in rabbit and found to be highly specific
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