pCambia1281Z

pCambia1281Z

pCambia 1281Z载体基本信息

出品公司:	Ybscience
载体名称:	pCAMBIA1281Z
质粒类型:	植物载体；农杆菌双元表达载体
高拷贝/低拷贝:	高拷贝
克隆方法:	限制性内切酶，多克隆位点
启动子:	lac
载体大小:	11379 bp
5' 测序引物及序列:	M13 Reverse:CAGGAAACAGCTATGAC
3' 测序引物及序列:	M13/pUC Forward:CCCAGTCACGACGTTGTAAAACG
载体标签:	GUS、6xHis
载体抗性:	氯霉素
筛选标记:	Hygromycin
克隆菌株:	HB101等菌株
宿主细胞（系）:	植物细胞、农杆菌
备注:	--
稳定性:	稳表达
组成型/诱导型:	诱导型
病毒/非病毒:	非病毒

 

pCambia 1281Z载体质粒图谱和多克隆位点信息

 

pCambia 1281Z载体简介

 

pCambia 1281Z载体序列

LOCUS       pCAMBIA1281Z          11379 bp ds-DNA    circular SYN 01-十二月-2013
DEFINITION  Agrobacterium binary vector for plant transformation, with 
            hygromycin- and chloramphenicol-resistance and LacZ-GUS genes plus 
            the pUC8 MCS.
ACCESSION   AF234294
VERSION     .
KEYWORDS    pCAMBIA1281Z
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
COMMENT     This file is created by Vector NTI
            http://www.biofeng.com/
COMMENT     VNTAUTHORNAME|biofeng.com|
COMMENT     The GenBank record was corrected by inserting a G at position 4743.
FEATURES             Location/Qualifiers
     source          1..11379
                     /organism="synthetic DNA construct"
                     /lab_host="Plant Cells"
                     /mol_type="other DNA"
     CDS             join(2..16,207..2024)
                     /codon_start=1
                     /product="beta-glucuronidase"
                     /label="GUS"
                     /label="This version of the gusA gene has a 5' extension 
                     with a catalase intron to ensure expression in plants but 
                     not bacteria."
                     /protein_id="
                     "                     
     intron          17..206
                     /label="cat1 intron"
                     /label="castor bean catalase intron, modified"
     CDS             2031..2048
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label="6xHis"
                     /translation="HHHHHH"
     terminator      2083..2335
                     /label="NOS terminator"
                     /label="nopaline synthase terminator and poly(A) signal"
     misc_feature    2357..2381
                     /label="RB T-DNA repeat"
                     /label="right border repeat from nopaline C58 T-DNA"
     CDS             3681..4310
                     /codon_start=1
                     /product="stability protein from Pseudomonas plasmid pVS1"
                     /label="pVS1 StaA"
     CDS             4739..5812
                     /codon_start=1
                     /product="replication protein from Pseudomonas plasmid 
                     pVS1"
                     /label="pVS1 RepA"
                     /label="
                     "
                     /protein_id="
                     "
     misc_feature    6416..6556
                     /label="bom"
                     /label="basis of mobility region from pBR322"
     rep_origin      complement(6742..7330)
                     /direction=LEFT
                     /label="ori"
                     /label="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(7601..8260)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label="CmR"
                     /label="confers resistance to chloramphenicol"
     misc_feature    8788..8812
                     /label="LB T-DNA repeat"
                     /label="left border repeat from nopaline C58 T-DNA"
     polyA_signal    8890..9064
                     /label="CaMV poly(A) signal"
                     /label="cauliflower mosaic virus polyadenylation signal"
     CDS             complement(9104..10129)
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label="HygR"
                     /label="confers resistance to hygromycin"
     promoter        complement(10197..10874)
                     /label="CaMV 35S promoter (enhanced)"
                     /label="cauliflower mosaic virus 35S promoter with a 
                     duplicated enhancer region"
     promoter        11088..11118
                     /label="lac promoter"
                     /label="promoter for the E. coli lac operon"
     protein_bind    11126..11142
                     /bound_moiety="lac repressor encoded by lacI"
                     /label="lac operator"
                     /label="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     11150..11166
                     /label="M13 rev"
                     /label="common sequencing primer, one of multiple similar 
                     variants"
     CDS             11162..11359
                     /codon_start=1
                     /gene="lacZ (fragment)"
                     /product="LacZ-alpha fragment of?beta-galactosidase"
                     /label="lacZ-alpha"
                     /protein_id="
                     "
     misc_feature    11176..11211
                     /label="MCS"
                     /label="multiple cloning site from pUC8"
     primer_bind     complement(11215..11231)
                     /label="M13 fwd"
                     /label="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 catggtagat ctgagggtaa atttctagtt tttctccttc attttcttgg ttaggaccct
       61 tttctctttt tatttttttg agctttgatc tttctttaaa ctgatctatt ttttaattga
      121 ttggttatgg tgtaaatatt acatagcttt aactgataat ctgattactt tatttcgtgt
      181 gtctatgatg atgatgatag ttacagaacc gacgactcgt ccgtcctgta gaaaccccaa
      241 cccgtgaaat caaaaaactc gacggcctgt gggcattcag tctggatcgc gaaaactgtg
      301 gaattgatca gcgttggtgg gaaagcgcgt tacaagaaag ccgggcaatt gctgtgccag
      361 gcagttttaa cgatcagttc gccgatgcag atattcgtaa ttatgcgggc aacgtctggt
      421 atcagcgcga agtctttata ccgaaaggtt gggcaggcca gcgtatcgtg ctgcgtttcg
      481 atgcggtcac tcattacggc aaagtgtggg tcaataatca ggaagtgatg gagcatcagg
      541 gcggctatac gccatttgaa gccgatgtca cgccgtatgt tattgccggg aaaagtgtac
      601 gtatcaccgt ttgtgtgaac aacgaactga actggcagac tatcccgccg ggaatggtga
      661 ttaccgacga aaacggcaag aaaaagcagt cttacttcca tgatttcttt aactatgccg
      721 gaatccatcg cagcgtaatg ctctacacca cgccgaacac ctgggtggac gatatcaccg
      781 tggtgacgca tgtcgcgcaa gactgtaacc acgcgtctgt tgactggcag gtggtggcca
      841 atggtgatgt cagcgttgaa ctgcgtgatg cggatcaaca ggtggttgca actggacaag
      901 gcactagcgg gactttgcaa gtggtgaatc cgcacctctg gcaaccgggt gaaggttatc
      961 tctatgaact cgaagtcaca gccaaaagcc agacagagtc tgatatctac ccgcttcgcg
     1021 tcggcatccg gtcagtggca gtgaagggcc aacagttcct gattaaccac aaaccgttct
     1081 actttactgg ctttggtcgt catgaagatg cggacttacg tggcaaagga ttcgataacg
     1141 tgctgatggt gcacgaccac gcattaatgg actggattgg ggccaactcc taccgtacct
     1201 cgcattaccc ttacgctgaa gagatgctcg actgggcaga tgaacatggc atcgtggtga
     1261 ttgatgaaac tgctgctgtc ggctttcagc tgtctttagg cattggtttc gaagcgggca
     1321 acaagccgaa agaactgtac agcgaagagg cagtcaacgg ggaaactcag caagcgcact
     1381 tacaggcgat taaagagctg atagcgcgtg acaaaaacca cccaagcgtg gtgatgtgga
     1441 gtattgccaa cgaaccggat acccgtccgc aaggtgcacg ggaatatttc gcgccactgg
     1501 cggaagcaac gcgtaaactc gacccgacgc gtccgatcac ctgcgtcaat gtaatgttct
     1561 gcgacgctca caccgatacc atcagcgatc tctttgatgt gctgtgcctg aaccgttatt
     1621 acggatggta tgtccaaagc ggcgatttgg aaacggcaga gaaggtactg gaaaaagaac
     1681 ttctggcctg gcaggagaaa ctgcatcagc cgattatcat caccgaatac ggcgtggata
     1741 cgttagccgg gctgcactca atgtacaccg acatgtggag tgaagagtat cagtgtgcat
     1801 ggctggatat gtatcaccgc gtctttgatc gcgtcagcgc cgtcgtcggt gaacaggtat
     1861 ggaatttcgc cgattttgcg acctcgcaag gcatattgcg cgttggcggt aacaagaaag
     1921 ggatcttcac tcgcgaccgc aaaccgaagt cggcggcttt tctgctgcaa aaacgctgga
     1981 ctggcatgaa cttcggtgaa aaaccgcagc agggaggcaa acaagctagc caccaccacc
     2041 accaccacgt gtgaattaca ggtgaccagc tcgaatttcc ccgatcgttc aaacatttgg
     2101 caataaagtt tcttaagatt gaatcctgtt gccggtcttg cgatgattat catataattt
     2161 ctgttgaatt acgttaagca tgtaataatt aacatgtaat gcatgacgtt atttatgaga
     2221 tgggttttta tgattagagt cccgcaatta tacatttaat acgcgataga aaacaaaata
     2281 tagcgcgcaa actaggataa attatcgcgc gcggtgtcat ctatgttact agatcgggaa
     2341 ttaaactatc agtgtttgac aggatatatt ggcgggtaaa cctaagagaa aagagcgttt
     2401 attagaataa cggatattta aaagggcgtg aaaaggttta tccgttcgtc catttgtatg
     2461 tgcatgccaa ccacagggtt cccctcggga tcaaagtact ttgatccaac ccctccgctg
     2521 ctatagtgca gtcggcttct gacgttcagt gcagccgtct tctgaaaacg acatgtcgca
     2581 caagtcctaa gttacgcgac aggctgccgc cctgcccttt tcctggcgtt ttcttgtcgc
     2641 gtgttttagt cgcataaagt agaatacttg cgactagaac cggagacatt acgccatgaa
     2701 caagagcgcc gccgctggcc tgctgggcta tgcccgcgtc agcaccgacg accaggactt
     2761 gaccaaccaa cgggccgaac tgcacgcggc cggctgcacc aagctgtttt ccgagaagat
     2821 caccggcacc aggcgcgacc gcccggagct ggccaggatg cttgaccacc tacgccctgg
     2881 cgacgttgtg acagtgacca ggctagaccg cctggcccgc agcacccgcg acctactgga
     2941 cattgccgag cgcatccagg aggccggcgc gggcctgcgt agcctggcag agccgtgggc
     3001 cgacaccacc acgccggccg gccgcatggt gttgaccgtg ttcgccggca ttgccgagtt
     3061 cgagcgttcc ctaatcatcg accgcacccg gagcgggcgc gaggccgcca aggcccgagg
     3121 cgtgaagttt ggcccccgcc ctaccctcac cccggcacag atcgcgcacg cccgcgagct
     3181 gatcgaccag gaaggccgca ccgtgaaaga ggcggctgca ctgcttggcg tgcatcgctc
     3241 gaccctgtac cgcgcacttg agcgcagcga ggaagtgacg cccaccgagg ccaggcggcg
     3301 cggtgccttc cgtgaggacg cattgaccga ggccgacgcc ctggcggccg ccgagaatga
     3361 acgccaagag gaacaagcat gaaaccgcac caggacggcc aggacgaacc gtttttcatt
     3421 accgaagaga tcgaggcgga gatgatcgcg gccgggtacg tgttcgagcc gcccgcgcac
     3481 gtctcaaccg tgcggctgca tgaaatcctg gccggtttgt ctgatgccaa gctggcggcc
     3541 tggccggcca gcttggccgc tgaagaaacc gagcgccgcc gtctaaaaag gtgatgtgta
     3601 tttgagtaaa acagcttgcg tcatgcggtc gctgcgtata tgatgcgatg agtaaataaa
     3661 caaatacgca aggggaacgc atgaaggtta tcgctgtact taaccagaaa ggcgggtcag
     3721 gcaagacgac catcgcaacc catctagccc gcgccctgca actcgccggg gccgatgttc
     3781 tgttagtcga ttccgatccc cagggcagtg cccgcgattg ggcggccgtg cgggaagatc
     3841 aaccgctaac cgttgtcggc atcgaccgcc cgacgattga ccgcgacgtg aaggccatcg
     3901 gccggcgcga cttcgtagtg atcgacggag cgccccaggc ggcggacttg gctgtgtccg
     3961 cgatcaaggc agccgacttc gtgctgattc cggtgcagcc aagcccttac gacatatggg
     4021 ccaccgccga cctggtggag ctggttaagc agcgcattga ggtcacggat ggaaggctac
     4081 aagcggcctt tgtcgtgtcg cgggcgatca aaggcacgcg catcggcggt gaggttgccg
     4141 aggcgctggc cgggtacgag ctgcccattc ttgagtcccg tatcacgcag cgcgtgagct
     4201 acccaggcac tgccgccgcc ggcacaaccg ttcttgaatc agaacccgag ggcgacgctg
     4261 cccgcgaggt ccaggcgctg gccgctgaaa ttaaatcaaa actcatttga gttaatgagg
     4321 taaagagaaa atgagcaaaa gcacaaacac gctaagtgcc ggccgtccga gcgcacgcag
     4381 cagcaaggct gcaacgttgg ccagcctggc agacacgcca gccatgaagc gggtcaactt
     4441 tcagttgccg gcggaggatc acaccaagct gaagatgtac gcggtacgcc aaggcaagac
     4501 cattaccgag ctgctatctg aatacatcgc gcagctacca gagtaaatga gcaaatgaat
     4561 aaatgagtag atgaatttta gcggctaaag gaggcggcat ggaaaatcaa gaacaaccag
     4621 gcaccgacgc cgtggaatgc cccatgtgtg gaggaacggg cggttggcca ggcgtaagcg
     4681 gctgggttgt ctgccggccc tgcaatggca ctggaacccc caagcccgag gaatcggcgt
     4741 gagcggtcgc aaaccatccg gcccggtaca aatcggcgcg gcgctgggtg atgacctggt
     4801 ggagaagttg aaggccgcgc aggccgccca gcggcaacgc atcgaggcag aagcacgccc
     4861 cggtgaatcg tggcaagcgg ccgctgatcg aatccgcaaa gaatcccggc aaccgccggc
     4921 agccggtgcg ccgtcgatta ggaagccgcc caagggcgac gagcaaccag attttttcgt
     4981 tccgatgctc tatgacgtgg gcacccgcga tagtcgcagc atcatggacg tggccgtttt
     5041 ccgtctgtcg aagcgtgacc gacgagctgg cgaggtgatc cgctacgagc ttccagacgg
     5101 gcacgtagag gtttccgcag ggccggccgg catggccagt gtgtgggatt acgacctggt
     5161 actgatggcg gtttcccatc taaccgaatc catgaaccga taccgggaag ggaagggaga
     5221 caagcccggc cgcgtgttcc gtccacacgt tgcggacgta ctcaagttct gccggcgagc
     5281 cgatggcgga aagcagaaag acgacctggt agaaacctgc attcggttaa acaccacgca
     5341 cgttgccatg cagcgtacga agaaggccaa gaacggccgc ctggtgacgg tatccgaggg
     5401 tgaagccttg attagccgct acaagatcgt aaagagcgaa accgggcggc cggagtacat
     5461 cgagatcgag ctagctgatt ggatgtaccg cgagatcaca gaaggcaaga acccggacgt
     5521 gctgacggtt caccccgatt actttttgat cgatcccggc atcggccgtt ttctctaccg
     5581 cctggcacgc cgcgccgcag gcaaggcaga agccagatgg ttgttcaaga cgatctacga
     5641 acgcagtggc agcgccggag agttcaagaa gttctgtttc accgtgcgca agctgatcgg
     5701 gtcaaatgac ctgccggagt acgatttgaa ggaggaggcg gggcaggctg gcccgatcct
     5761 agtcatgcgc taccgcaacc tgatcgaggg cgaagcatcc gccggttcct aatgtacgga
     5821 gcagatgcta gggcaaattg ccctagcagg ggaaaaaggt cgaaaaggtc tctttcctgt
     5881 ggatagcacg tacattggga acccaaagcc gtacattggg aaccggaacc cgtacattgg
     5941 gaacccaaag ccgtacattg ggaaccggtc acacatgtaa gtgactgata taaaagagaa
     6001 aaaaggcgat ttttccgcct aaaactcttt aaaacttatt aaaactctta aaacccgcct
     6061 ggcctgtgca taactgtctg gccagcgcac agccgaagag ctgcaaaaag cgcctaccct
     6121 tcggtcgctg cgctccctac gccccgccgc ttcgcgtcgg cctatcgcgg ccgctggccg
     6181 ctcaaaaatg gctggcctac ggccaggcaa tctaccaggg cgcggacaag ccgcgccgtc
     6241 gccactcgac cgccggcgcc cacatcaagg caccctgcct cgcgcgtttc ggtgatgacg
     6301 gtgaaaacct ctgacacatg cagctcccgg agacggtcac agcttgtctg taagcggatg
     6361 ccgggagcag acaagcccgt cagggcgcgt cagcgggtgt tggcgggtgt cggggcgcag
     6421 ccatgaccca gtcacgtagc gatagcggag tgtatactgg cttaactatg cggcatcaga
     6481 gcagattgta ctgagagtgc accatatgcg gtgtgaaata ccgcacagat gcgtaaggag
     6541 aaaataccgc atcaggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt
     6601 tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc
     6661 aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa
     6721 aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa
     6781 tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc
     6841 ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc
     6901 cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag
     6961 ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga
     7021 ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc
     7081 gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac
     7141 agagttcttg aagtggtggc ctaactacgg ctacactaga aggacagtat ttggtatctg
     7201 cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca
     7261 aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa
     7321 aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa
     7381 ctcacgttaa gggattttgg tcatgcatga tatatctccc aatttgtgta gggcttatta
     7441 tgcacgctta aaaataataa aagcagactt gacctgatag tttggctgtg agcaattatg
     7501 tgcttagtgc atctaatcgc ttgagttaac gccggcgaag cggcgtcggc ttgaacgaat
     7561 ttctagctag aggatcgcac caataactgc cttaaaaaaa ttacgccccg ccctgccact
     7621 catcgcagta ctgttgtaat tcattaagca ttctgccgac atggaagcca tcacaaacgg
     7681 catgatgaac ctgaatcgcc agcggcatca gcaccttgtc gccttgcgta taatatttgc
     7741 ccattgtgaa aacgggggcg aagaagttgt ccatattggc cacgtttaaa tcaaaactgg
     7801 tgaaactcac ccagggattg gctgagacga aaaacatatt ctcaataaac cctttaggga
     7861 aataggccag gttttcaccg taacacgcca catcttgcga atatatgtgt agaaactgcc
     7921 ggaaatcgtc gtggtattca ctccagagcg atgaaaacgt ttcagtttgc tcatggaaaa
     7981 cggtgtaaca agggtgaaca ctatcccata tcaccagctc accgtctttc attgccatac
     8041 ggaactccgg atgagcattc atcaggcggg caagaatgtg aataaaggcc ggataaaact
     8101 tgtgcttatt tttctttacg gtctttaaaa aggccgtaat atccagctga acggtctggt
     8161 tataggtaca ttgagcaact gactgaaatg cctcaaaatg ttctttacga tgccattggg
     8221 atatatcaac ggtggtatat ccagtgattt ttttctccat gatgtttaac tttgttttag
     8281 ggcgactgcc ctgctgcgta acatcgttgc tgctccataa catcaaacat cgacccacgg
     8341 cgtaacgcgc ttgctgcttg gatgcccgag gcatagactg taccccaaaa aaacatgtca
     8401 taacaagaag ccatgaaaac cgccactgcg ccgttaccac cgctgcgttc ggtcaaggtt
     8461 ctggaccagt tgcgtgacgg cagttacgct acttgcatta cagcttacga accgaacgag
     8521 gcttatgtcc actgggttcg tgcccgaatt gatcacaggc agcaacgctc tgtcatcgtt
     8581 acaatcaaca tgctaccctc cgcgagatca tccgtgtttc aaacccggca gcttagttgc
     8641 cgttcttccg aatagcatcg gtaacatgag caaagtctgc cgccttacaa cggctctccc
     8701 gctgacgccg tcccggactg atgggctgcc tgtatcgagt ggtgattttg tgccgagctg
     8761 ccggtcgggg agctgttggc tggctggtgg caggatatat tgtggtgtaa acaaattgac
     8821 gcttagacaa cttaataaca cattgcggac gtttttaatg tactgaatta acgccgaatt
     8881 aattcggggg atctggattt tagtactgga ttttggtttt aggaattaga aattttattg
     8941 atagaagtat tttacaaata caaatacata ctaagggttt cttatatgct caacacatga
     9001 gcgaaaccct ataggaaccc taattccctt atctgggaac tactcacaca ttattatgga
     9061 gaaactcgag cttgtcgatc gacagatccg gtcggcatct actctatttc tttgccctcg
     9121 gacgagtgct ggggcgtcgg tttccactat cggcgagtac ttctacacag ccatcggtcc
     9181 agacggccgc gcttctgcgg gcgatttgtg tacgcccgac agtcccggct ccggatcgga
     9241 cgattgcgtc gcatcgaccc tgcgcccaag ctgcatcatc gaaattgccg tcaaccaagc
     9301 tctgatagag ttggtcaaga ccaatgcgga gcatatacgc ccggagtcgt ggcgatcctg
     9361 caagctccgg atgcctccgc tcgaagtagc gcgtctgctg ctccatacaa gccaaccacg
     9421 gcctccagaa gaagatgttg gcgacctcgt attgggaatc cccgaacatc gcctcgctcc
     9481 agtcaatgac cgctgttatg cggccattgt ccgtcaggac attgttggag ccgaaatccg
     9541 cgtgcacgag gtgccggact tcggggcagt cctcggccca aagcatcagc tcatcgagag
     9601 cctgcgcgac ggacgcactg acggtgtcgt ccatcacagt ttgccagtga tacacatggg
     9661 gatcagcaat cgcgcatatg aaatcacgcc atgtagtgta ttgaccgatt ccttgcggtc
     9721 cgaatgggcc gaacccgctc gtctggctaa gatcggccgc agcgatcgca tccatagcct
     9781 ccgcgaccgg ttgtagaaca gcgggcagtt cggtttcagg caggtcttgc aacgtgacac
     9841 cctgtgcacg gcgggagatg caataggtca ggctctcgct aaactcccca atgtcaagca
     9901 cttccggaat cgggagcgcg gccgatgcaa agtgccgata aacataacga tctttgtaga
     9961 aaccatcggc gcagctattt acccgcagga catatccacg ccctcctaca tcgaagctga
    10021 aagcacgaga ttcttcgccc tccgagagct gcatcaggtc ggagacgctg tcgaactttt
    10081 cgatcagaaa cttctcgaca gacgtcgcgg tgagttcagg ctttttcata tctcattgcc
    10141 ccccgggatc tgcgaaagct cgagagagat agatttgtag agagagactg gtgatttcag
    10201 cgtgtcctct ccaaatgaaa tgaacttcct tatatagagg aaggtcttgc gaaggatagt
    10261 gggattgtgc gtcatccctt acgtcagtgg agatatcaca tcaatccact tgctttgaag
    10321 acgtggttgg aacgtcttct ttttccacga tgctcctcgt gggtgggggt ccatctttgg
    10381 gaccactgtc ggcagaggca tcttgaacga tagcctttcc tttatcgcaa tgatggcatt
    10441 tgtaggtgcc accttccttt tctactgtcc ttttgatgaa gtgacagata gctgggcaat
    10501 ggaatccgag gaggtttccc gatattaccc tttgttgaaa agtctcaata gccctttggt
    10561 cttctgagac tgtatctttg atattcttgg agtagacgag agtgtcgtgc tccaccatgt
    10621 tatcacatca atccacttgc tttgaagacg tggttggaac gtcttctttt tccacgatgc
    10681 tcctcgtggg tgggggtcca tctttgggac cactgtcggc agaggcatct tgaacgatag
    10741 cctttccttt atcgcaatga tggcatttgt aggtgccacc ttccttttct actgtccttt
    10801 tgatgaagtg acagatagct gggcaatgga atccgaggag gtttcccgat attacccttt
    10861 gttgaaaagt ctcaatagcc ctttggtctt ctgagactgt atctttgata ttcttggagt
    10921 agacgagagt gtcgtgctcc accatgttgg ccaatacgca aaccgcctct ccccgcgcgt
    10981 tggccgattc attaatgcag ctggcacgac aggtttcccg actggaaagc gggcagtgag
    11041 cgcaacgcaa ttaatgtgag ttagctcact cattaggcac cccaggcttt acactttatg
    11101 cttccggctc gtatgttgtg tggaattgtg agcggataac aatttcacac aggaaacagc
    11161 tatgaccatg attacgaatt cccggggatc cgtcgacctg cagccaagct tggcactggc
    11221 cgtcgtttta caacgtcgtg actgggaaaa ccctggcgtt acccaactta atcgccttgc
    11281 agcacatccc cctttcgcca gctggcgtaa tagcgaagag gcccgcaccg atcgcccttc
    11341 ccaacagttg cgcagcctga agcctaggga ggagtccac