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- 详细信息
- 文献和实验
- 技术资料
- 库存：
大量
- 英文名：
pCambia0380
- 保质期：
6个月
- 供应商：
上海钰博生物科技有限公司
- 保存条件：
-20℃
- 规格：
1ug/5ug
pCambia0380
| 产品编号 |
载体名称 |
| YB00505 | pCambia0380 |
pCambia0380载体基本信息
| 载体名称: | pCambia0380 |
|---|---|
| 质粒类型: | 植物表达载体 |
| 高拷贝/低拷贝: | 低拷贝 |
| 启动子: | -- |
| 克隆方法: | 多克隆位点，限制性内切酶 |
| 载体大小: | 6812 bp |
| 5' 测序引物及序列: | -- |
| 3' 测序引物及序列: | -- |
| 载体标签: | -- |
| 载体抗性: | 卡那 |
| 筛选标记: | -- |
| 备注: | -- |
| 稳定性: | 稳定表达 |
| 组成型: | 非组成型 |
| 病毒/非病毒: | 非病毒 |
pCambia0380载体质粒图谱和多克隆位点信息
pCambia0380载体序列
LOCUS pCambia0380 6813 bp ds-DNA circular SYN DEFINITION Agrobacterium binary vector for plant transformation, with a kanamycin-resistance gene. The MCS is reversed in pCAMBIA0390. ACCESSION AF234290 VERSION . KEYWORDS pCAMBIA0380 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct COMMENT This file is created by Vector NTI COMMENT The GenBank record was corrected by inserting a G at position 2718. FEATURES Location/Qualifiers source 1..6813 /organism="synthetic DNA construct" /lab_host="Plant Cells" /mol_type="other DNA" CDS 6..23 /codon_start=1 /product="6xHis affinity tag" /label="6xHis" /translation="HHHHHH" terminator 58..310 /label="NOS terminator" /label="nopaline synthase terminator and poly(A) signal" misc_feature 332..356 /label="RB T-DNA repeat" /label="right border repeat from nopaline C58 T-DNA" CDS 1656..2285 /codon_start=1 /product="stability protein from Pseudomonas plasmid pVS1" /label="pVS1 StaA" CDS 2714..3787 /codon_start=1 /product="replication protein from Pseudomonas plasmid pVS1" /label="pVS1 RepA" misc_feature 4391..4531 /label="bom" /label="basis of mobility region from pBR322" rep_origin complement(4717..5305) /direction=LEFT /label="ori" /label="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" CDS complement(5392..6186) /codon_start=1 /gene="aphA-3" /product="aminoglycoside phosphotransferase" /label="KanR" /label="confers resistance to kanamycin" misc_feature 6611..6635 /label="LB T-DNA repeat" /label="left border repeat from nopaline C58 T-DNA" misc_feature 6738..6773 /label="MCS" /label="multiple cloning site from pUC8" ORIGIN 1 ctagccacca ccaccaccac cacgtgtgaa ttacaggtga ccagctcgaa tttccccgat 61 cgttcaaaca tttggcaata aagtttctta agattgaatc ctgttgccgg tcttgcgatg 121 attatcatat aatttctgtt gaattacgtt aagcatgtaa taattaacat gtaatgcatg 181 acgttattta tgagatgggt ttttatgatt agagtcccgc aattatacat ttaatacgcg 241 atagaaaaca aaatatagcg cgcaaactag gataaattat cgcgcgcggt gtcatctatg 301 ttactagatc gggaattaaa ctatcagtgt ttgacaggat atattggcgg gtaaacctaa 361 gagaaaagag cgtttattag aataacggat atttaaaagg gcgtgaaaag gtttatccgt 421 tcgtccattt gtatgtgcat gccaaccaca gggttcccct cgggatcaaa gtactttgat 481 ccaacccctc cgctgctata gtgcagtcgg cttctgacgt tcagtgcagc cgtcttctga 541 aaacgacatg tcgcacaagt cctaagttac gcgacaggct gccgccctgc ccttttcctg 601 gcgttttctt gtcgcgtgtt ttagtcgcat aaagtagaat acttgcgact agaaccggag 661 acattacgcc atgaacaaga gcgccgccgc tggcctgctg ggctatgccc gcgtcagcac 721 cgacgaccag gacttgacca accaacgggc cgaactgcac gcggccggct gcaccaagct 781 gttttccgag aagatcaccg gcaccaggcg cgaccgcccg gagctggcca ggatgcttga 841 ccacctacgc cctggcgacg ttgtgacagt gaccaggcta gaccgcctgg cccgcagcac 901 ccgcgaccta ctggacattg ccgagcgcat ccaggaggcc ggcgcgggcc tgcgtagcct 961 ggcagagccg tgggccgaca ccaccacgcc ggccggccgc atggtgttga ccgtgttcgc 1021 cggcattgcc gagttcgagc gttccctaat catcgaccgc acccggagcg ggcgcgaggc 1081 cgccaaggcc cgaggcgtga agtttggccc ccgccctacc ctcaccccgg cacagatcgc 1141 gcacgcccgc gagctgatcg accaggaagg ccgcaccgtg aaagaggcgg ctgcactgct 1201 tggcgtgcat cgctcgaccc tgtaccgcgc acttgagcgc agcgaggaag tgacgcccac 1261 cgaggccagg cggcgcggtg ccttccgtga ggacgcattg accgaggccg acgccctggc 1321 ggccgccgag aatgaacgcc aagaggaaca agcatgaaac cgcaccagga cggccaggac 1381 gaaccgtttt tcattaccga agagatcgag gcggagatga tcgcggccgg gtacgtgttc 1441 gagccgcccg cgcacgtctc aaccgtgcgg ctgcatgaaa tcctggccgg tttgtctgat 1501 gccaagctgg cggcctggcc ggccagcttg gccgctgaag aaaccgagcg ccgccgtcta 1561 aaaaggtgat gtgtatttga gtaaaacagc ttgcgtcatg cggtcgctgc gtatatgatg 1621 cgatgagtaa ataaacaaat acgcaagggg aacgcatgaa ggttatcgct gtacttaacc 1681 agaaaggcgg gtcaggcaag acgaccatcg caacccatct agcccgcgcc ctgcaactcg 1741 ccggggccga tgttctgtta gtcgattccg atccccaggg cagtgcccgc gattgggcgg 1801 ccgtgcggga agatcaaccg ctaaccgttg tcggcatcga ccgcccgacg attgaccgcg 1861 acgtgaaggc catcggccgg cgcgacttcg tagtgatcga cggagcgccc caggcggcgg 1921 acttggctgt gtccgcgatc aaggcagccg acttcgtgct gattccggtg cagccaagcc 1981 cttacgacat atgggccacc gccgacctgg tggagctggt taagcagcgc attgaggtca 2041 cggatggaag gctacaagcg gcctttgtcg tgtcgcgggc gatcaaaggc acgcgcatcg 2101 gcggtgaggt tgccgaggcg ctggccgggt acgagctgcc cattcttgag tcccgtatca 2161 cgcagcgcgt gagctaccca ggcactgccg ccgccggcac aaccgttctt gaatcagaac 2221 ccgagggcga cgctgcccgc gaggtccagg cgctggccgc tgaaattaaa tcaaaactca 2281 tttgagttaa tgaggtaaag agaaaatgag caaaagcaca aacacgctaa gtgccggccg 2341 tccgagcgca cgcagcagca aggctgcaac gttggccagc ctggcagaca cgccagccat 2401 gaagcgggtc aactttcagt tgccggcgga ggatcacacc aagctgaaga tgtacgcggt 2461 acgccaaggc aagaccatta ccgagctgct atctgaatac atcgcgcagc taccagagta 2521 aatgagcaaa tgaataaatg agtagatgaa ttttagcggc taaaggaggc ggcatggaaa 2581 atcaagaaca accaggcacc gacgccgtgg aatgccccat gtgtggagga acgggcggtt 2641 ggccaggcgt aagcggctgg gttgtctgcc ggccctgcaa tggcactgga acccccaagc 2701 ccgaggaatc ggcgtgagcg gtcgcaaacc atccggcccg gtacaaatcg gcgcggcgct 2761 gggtgatgac ctggtggaga agttgaaggc cgcgcaggcc gcccagcggc aacgcatcga 2821 ggcagaagca cgccccggtg aatcgtggca agcggccgct gatcgaatcc gcaaagaatc 2881 ccggcaaccg ccggcagccg gtgcgccgtc gattaggaag ccgcccaagg gcgacgagca 2941 accagatttt ttcgttccga tgctctatga cgtgggcacc cgcgatagtc gcagcatcat 3001 ggacgtggcc gttttccgtc tgtcgaagcg tgaccgacga gctggcgagg tgatccgcta 3061 cgagcttcca gacgggcacg tagaggtttc cgcagggccg gccggcatgg ccagtgtgtg 3121 ggattacgac ctggtactga tggcggtttc ccatctaacc gaatccatga accgataccg 3181 ggaagggaag ggagacaagc ccggccgcgt gttccgtcca cacgttgcgg acgtactcaa 3241 gttctgccgg cgagccgatg gcggaaagca gaaagacgac ctggtagaaa cctgcattcg 3301 gttaaacacc acgcacgttg ccatgcagcg tacgaagaag gccaagaacg gccgcctggt 3361 gacggtatcc gagggtgaag ccttgattag ccgctacaag atcgtaaaga gcgaaaccgg 3421 gcggccggag tacatcgaga tcgagctagc tgattggatg taccgcgaga tcacagaagg 3481 caagaacccg gacgtgctga cggttcaccc cgattacttt ttgatcgatc ccggcatcgg 3541 ccgttttctc taccgcctgg cacgccgcgc cgcaggcaag gcagaagcca gatggttgtt 3601 caagacgatc tacgaacgca gtggcagcgc cggagagttc aagaagttct gtttcaccgt 3661 gcgcaagctg atcgggtcaa atgacctgcc ggagtacgat ttgaaggagg aggcggggca 3721 ggctggcccg atcctagtca tgcgctaccg caacctgatc gagggcgaag catccgccgg 3781 ttcctaatgt acggagcaga tgctagggca aattgcccta gcaggggaaa aaggtcgaaa 3841 aggtctcttt cctgtggata gcacgtacat tgggaaccca aagccgtaca ttgggaaccg 3901 gaacccgtac attgggaacc caaagccgta cattgggaac cggtcacaca tgtaagtgac 3961 tgatataaaa gagaaaaaag gcgatttttc cgcctaaaac tctttaaaac ttattaaaac 4021 tcttaaaacc cgcctggcct gtgcataact gtctggccag cgcacagccg aagagctgca 4081 aaaagcgcct acccttcggt cgctgcgctc cctacgcccc gccgcttcgc gtcggcctat 4141 cgcggccgct ggccgctcaa aaatggctgg cctacggcca ggcaatctac cagggcgcgg 4201 acaagccgcg ccgtcgccac tcgaccgccg gcgcccacat caaggcaccc tgcctcgcgc 4261 gtttcggtga tgacggtgaa aacctctgac acatgcagct cccggagacg gtcacagctt 4321 gtctgtaagc ggatgccggg agcagacaag cccgtcaggg cgcgtcagcg ggtgttggcg 4381 ggtgtcgggg cgcagccatg acccagtcac gtagcgatag cggagtgtat actggcttaa 4441 ctatgcggca tcagagcaga ttgtactgag agtgcaccat atgcggtgtg aaataccgca 4501 cagatgcgta aggagaaaat accgcatcag gcgctcttcc gcttcctcgc tcactgactc 4561 gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg 4621 gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa 4681 ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga 4741 cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag 4801 ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct 4861 taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg 4921 ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc 4981 ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt 5041 aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta 5101 tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaaggac 5161 agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc 5221 ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat 5281 tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc 5341 tcagtggaac gaaaactcac gttaagggat tttggtcatg cattctaggt actaaaacaa 5401 ttcatccagt aaaatataat attttatttt ctcccaatca ggcttgatcc ccagtaagtc 5461 aaaaaatagc tcgacatact gttcttcccc gatatcctcc ctgatcgacc ggacgcagaa 5521 ggcaatgtca taccacttgt ccgccctgcc gcttctccca agatcaataa agccacttac 5581 tttgccatct ttcacaaaga tgttgctgtc tcccaggtcg ccgtgggaaa agacaagttc 5641 ctcttcgggc ttttccgtct ttaaaaaatc atacagctcg cgcggatctt taaatggagt 5701 gtcttcttcc cagttttcgc aatccacatc ggccagatcg ttattcagta agtaatccaa 5761 ttcggctaag cggctgtcta agctattcgt atagggacaa tccgatatgt cgatggagtg 5821 aaagagcctg atgcactccg catacagctc gataatcttt tcagggcttt gttcatcttc 5881 atactcttcc gagcaaagga cgccatcggc ctcactcatg agcagattgc tccagccatc 5941 atgccgttca aagtgcagga cctttggaac aggcagcttt ccttccagcc atagcatcat 6001 gtccttttcc cgttccacat cataggtggt ccctttatac cggctgtccg tcatttttaa 6061 atataggttt tcattttctc ccaccagctt atatacctta gcaggagaca ttccttccgt 6121 atcttttacg cagcggtatt tttcgatcag ttttttcaat tccggtgata ttctcatttt 6181 agccatttat tatttccttc ctcttttcta cagtatttaa agatacccca agaagctaat 6241 tataacaaga cgaactccaa ttcactgttc cttgcattct aaaaccttaa ataccagaaa 6301 acagcttttt caaagttgtt ttcaaagttg gcgtataaca tagtatcgac ggagccgatt 6361 ttgaaaccgc ggtgatcaca ggcagcaacg ctctgtcatc gttacaatca acatgctacc 6421 ctccgcgaga tcatccgtgt ttcaaacccg gcagcttagt tgccgttctt ccgaatagca 6481 tcggtaacat gagcaaagtc tgccgcctta caacggctct cccgctgacg ccgtcccgga 6541 ctgatgggct gcctgtatcg agtggtgatt ttgtgccgag ctgccggtcg gggagctgtt 6601 ggctggctgg tggcaggata tattgtggtg taaacaaatt gacgcttaga caacttaata 6661 acacattgcg gacgttttta atgtactgaa ttaacgccga attaattcct aggccaccat 6721 gttgggcccg gcgcgccgaa ttcccgggga tccgtcgacc tgcagccaag ctttaagagg 6781 agtccaccat ggtagatctg actagtgtta acg //
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文献和实验相关实验
-DNA or transposon) insertions using the TAIL-PCR method. The amplified products are suitable as templates for direct sequencing, or for cloning in vectors. Examples are given in use of the protocol for T-DNA tagging in rice using the pCAMBIA binary vectors.
plants, bypassing the callus formation phase. Stolon nodes are infected and co-cultivated with Agrobacterium tumefaciens harboring pCAMBIA binary vectors. Hygromycin phosphotransferase gene (hph ) is used as the selectable marker and hygromycin is used
downstream of the stop codon, was amplified from genomic DNA and cloned into a binary vector pCAMBIA 1301, then transformed into fon4-1 by Agrobacterium-mediated transformation (Hiei et al., 1994). From: Huangwei Chu, Qian Qian, Wanqi Liang, Changsong Yin
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pCambia0380
¥500 - 1580
