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- 保存条件：
-20℃
- 保质期：
6个月
- 英文名：
pTrcHis2 B
- 库存：
大量
- 供应商：
钰博生物
- 规格：
1ug/5ug
pTrcHis2B载体基本信息
| 出品公司: | Ybscience |
|---|---|
| 别名: | pTrcHis2B、 pTrcHis2 B |
| 质粒类型: | 大肠杆菌表达载体 |
| 表达水平: | 高拷贝 |
| 启动子: | Trc |
| 克隆方法: | 多克隆位点，限制性内切酶 |
| 载体大小: | 4404 bp |
| 5' 测序引物及序列: | pTrcHis-F: gaggtatatattaatgtatcg |
| 3' 测序引物及序列: | pTrcHis-R:GATTTAATCTGTATCAGG |
| 载体标签: | C-His, C-Myc |
| 载体抗性: | Ampicillin |
| 备注: | Hosts: E.coli NM522, E.coli INValphaF'. Related vectors: pSE420. |
| 稳定性: | 瞬时表达 Transient |
| 组成型: | 组成型 Constitutive |
| 病毒/非病毒: | 非病毒 |
pTrcHis2B载体质粒图谱和多克隆位点信息
pTrcHis2B载体简介
The pTrcHis2 plasmids are pBR322-derived expression vectors designed for efficient recombinant protein expression and purification in E. coli. High levels of expression are possible using the trc (trp-lac) promoter (Egon et al., 1983) and the rrnB anti-termination region (Li et al., 1984). The trc promoter contains the –35 region of the trp promoter together with the –10 region of the lac promoter (Brosius et al., 1985; Egon et al., 1983; Mulligan et al., 1985). To regulate expression, the gene encoding Lac repressor (lacIq) is provided in the pTrcHis2 vectors, allowing regulation of the trc promoter regardless of whether the host strain contains a gene encoding the Lac repressor.
Isopropyl-Beta-D-thiogalactopyranoside (IPTG) is used to induce expression of your gene. Translation is enhanced by the bacteriophage T7 gene 10 translation enhancer and a minicistron that provides highly efficient translational restart into the open reading frame of the multiple cloning site. DNA inserts are positioned downstream and in frame with the initiation ATG and a C-terminal fusion peptide. The C-terminal peptide encodes the myc epitope and six histidine residues that function as a metal binding site in the expressed protein.
pTrcHis2B载体序列
LOCUS pTrcHis2\B 4404 bp DNA circular SYN 7-JUL-2013DEFINITION pTrcHis2 B.
SOURCE
ORGANISM other sequences; artificial sequences; vectors.
COMMENT http://www.biofeng.com/
COMMENT This file is created by Vector NTI
http://www.invitrogen.com/
COMMENT ORIGDB|GenBank
COMMENT VNTNAME|pTrcHis2 B|
COMMENT VNTOAUTHORNAME|biofeng.com|
FEATURES Location/Qualifiers
source 1..4404
/organism="pTrcHis2 A"
/mol_type="other DNA"
/vntifkey="98"
misc_feature 235..257
/vntifkey="21"
/label=M13_pUC_rev_primer
misc_feature 264..333
/vntifkey="21"
/label=rrnB_ATS
misc_feature 370..390
/vntifkey="21"
/label=pTrcHis_fwd_primer
misc_feature 383..409
/vntifkey="21"
/label=minicis
misc_feature complement(587..604)
/vntifkey="21"
/label=pBAD_rev_primer
misc_feature complement(587..604)
/vntifkey="21"
/label=pTrcHis_rev_primer
terminator 637..794
/vntifkey="43"
/label=rrnB_terminator
terminator 760..803
/vntifkey="43"
/label=rrnB_T1_terminator
terminator 935..962
/vntifkey="43"
/label=rrnB_T2_terminator
promoter 1004..1032
/vntifkey="30"
/label=AmpR_promoter
gene 1074..1934
/gene="Ampicillin"
/vntifkey="60"
CDS 1074..1934
/vntifkey="4"
/label=ORF\frame\2
rep_origin 2089..2708
/vntifkey="33"
/label=pBR322_origin
misc_feature 3105..3127
/vntifkey="21"
/label=pGEX_3_primer
misc_feature 3274..4365
/vntifkey="21"
/label=lacI
CDS 3406..4365
/vntifkey="4"
/label=ORF\frame\3
BASE COUNT 1091 a 1098 c 1160 g 1055 t
ORIGIN
1 gtttgacagc ttatcatcga ctgcacggtg caccaatgct tctggcgtca ggcagccatc
61 ggaagctgtg gtatggctgt gcaggtcgta aatcactgca taattcgtgt cgctcaaggc
121 gcactcccgt tctggataat gttttttgcg ccgacatcat aacggttctg gcaaatattc
181 tgaaatgagc tgttgacaat taatcatccg gctcgtataa tgtgtggaat tgtgagcgga
241 taacaatttc acacaggaaa cagcgccgct gagaaaaagc gaagcggcac tgctctttaa
301 caatttatca gacaatctgt gtgggcactc gaccggaatt atcgattaac tttattatta
361 aaaattaaag aggtatatat taatgtatcg attaaataag gaggaataaa ccatggatcc
421 gagctcgaga tctgcagctg gtaccatatg ggaattcgaa gctttctaga acaaaaactc
481 atctcagaag aggatctgaa tagcgccgtc gaccatcatc atcatcatca ttgagtttaa
541 acggtctcca gcttggctgt tttggcggat gagagaagat tttcagcctg atacagatta
601 aatcagaacg cagaagcggt ctgataaaac agaatttgcc tggcggcagt agcgcggtgg
661 tcccacctga ccccatgccg aactcagaag tgaaacgccg tagcgccgat ggtagtgtgg
721 ggtctcccca tgcgagagta gggaactgcc aggcatcaaa taaaacgaaa ggctcagtcg
781 aaagactggg cctttcgttt tatctgttgt ttgtcggtga acgctctcct gagtaggaca
841 aatccgccgg gagcggattt gaacgttgcg aagcaacggc ccggagggtg gcgggcagga
901 cgcccgccat aaactgccag gcatcaaatt aagcagaagg ccatcctgac ggatggcctt
961 tttgcgtttc tacaaactct ttttgtttat ttttctaaat acattcaaat atgtatccgc
1021 tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta
1081 ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg
1141 ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg
1201 gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac
1261 gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtgttg
1321 acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt
1381 actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg
1441 ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac
1501 cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt
1561 gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgtag
1621 caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc
1681 aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc
1741 ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta
1801 tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg
1861 ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga
1921 ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac
1981 ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa
2041 tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat
2101 cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc
2161 taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg
2221 gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc
2281 acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg
2341 ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg
2401 ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa
2461 cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg
2521 aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga
2581 gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct
2641 gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca
2701 gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc
2761 ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg
2821 ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc
2881 tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata tggtgcactc
2941 tcagtacaat ctgctctgat gccgcatagt taagccagta tacactccgc tatcgctacg
3001 tgactgggtc atggctgcgc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc
3061 ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga gctgcatgtg
3121 tcagaggttt tcaccgtcat caccgaaacg cgcgaggcag cagatcaatt cgcgcgcgaa
3181 ggcgaagcgg catgcattta cgttgacacc atcgaatggt gcaaaacctt tcgcggtatg
3241 gcatgatagc gcccggaaga gagtcaattc agggtggtga atgtgaaacc agtaacgtta
3301 tacgatgtcg cagagtatgc cggtgtctct tatcagaccg tttcccgcgt ggtgaaccag
3361 gccagccacg tttctgcgaa aacgcgggaa aaagtggaag cggcgatggc ggagctgaat
3421 tacattccca accgcgtggc acaacaactg gcgggcaaac agtcgttgct gattggcgtt
3481 gccacctcca gtctggccct gcacgcgccg tcgcaaattg tcgcggcgat taaatctcgc
3541 gccgatcaac tgggtgccag cgtggtggtg tcgatggtag aacgaagcgg cgtcgaagcc
3601 tgtaaagcgg cggtgcacaa tcttctcgcg caacgcgtca gtgggctgat cattaactat
3661 ccgctggatg accaggatgc cattgctgtg gaagctgcct gcactaatgt tccggcgtta
3721 tttcttgatg tctctgacca gacacccatc aacagtatta ttttctccca tgaagacggt
3781 acgcgactgg gcgtggagca tctggtcgca ttgggtcacc agcaaatcgc gctgttagcg
3841 ggcccattaa gttctgtctc ggcgcgtctg cgtctggctg gctggcataa atatctcact
3901 cgcaatcaaa ttcagccgat agcggaacgg gaaggcgact ggagtgccat gtccggtttt
3961 caacaaacca tgcaaatgct gaatgagggc atcgttccca ctgcgatgct ggttgccaac
4021 gatcagatgg cgctgggcgc aatgcgcgcc attaccgagt ccgggctgcg cgttggtgcg
4081 gatatctcgg tagtgggata cgacgatacc gaagacagct catgttatat cccgccgtca
4141 accaccatca aacaggattt tcgcctgctg gggcaaacca gcgtggaccg cttgctgcaa
4201 ctctctcagg gccaggcggt gaagggcaat cagctgttgc ccgtctcact ggtgaaaaga
4261 aaaaccaccc tggcgcccaa tacgcaaacc gcctctcccc gcgcgttggc cgattcatta
4321 atgcagctgg cacgacaggt ttcccgactg gaaagcgggc agtgagcgca acgcaattaa
4381 tgtgagttag cgcgaattga tctg
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文献和实验第一个用于研究细胞骨架的药物，它是真菌分泌的生物碱。细胞松弛素(细胞松弛素 B 及其衍生物)在细胞内同微丝的正端结合, 并引起F-肌动蛋白解聚，阻断亚基的进一步聚合。当将细胞松弛素加入到活细胞后，肌动蛋白纤维骨架消失，使动物细胞的各种活动瘫痪, 包括细胞的移动、吞噬作用、胞质分裂等。它对微管没有作用, 也不抑制肌收缩, 因肌纤维中肌动蛋白丝是稳定的结构, 不发生组装及解聚的动态平衡。
化，其他已知细胞松弛素 B还可抑制糖的输送，可以考虑细胞松弛素 B与细胞膜间的相互作用。此外还分离出数种具有类似结构和功能的物质，分别命名为细胞松弛素 A、 C、 D、 E、 F。
细胞色素b563 cytochrome b563 亦称细胞色素 b6 。存在于高等植物至藻类的叶绿体中，与类囊体膜结合紧密。含有原正铁血红素，氧化还原 （β带）和 434nm（γ带或 So－ ret带）。为发动光合磷酸化的环状电子传递系统的一个成分，可被光化系统Ⅰ所还原和氧化。
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