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- 详细信息
- 文献和实验
- 技术资料
- 英文名:
FastDigest Eco72I
- 库存:
大量
- 供应商:
上海研卉生物科技
- 规格:
200 reactions
描述
| 5' | C | A | C ↓ | G | T | G | 3' | ||||
| 3' | G | T | G ↑ | C | A | C | 5' |
Thermo Scientific FastDigest Eco72I restriction enzyme recognizes CAC^GTG site and cuts best at 37°C in 5–15 minutes using universal FastDigest Buffer. Isoschizomers: PmlI, AcvI, BbrPI, PmaCI, PspCI.
Thermo Scientific FastDigest Eco72I is one of an advanced line of fast restriction enzymes that are all 100% active in the universal FastDigest and FastDigest Green reaction buffers.
The universal buffer allows rapid single-, double-, or multiple DNA digestion within 5–15 minutes eliminating any need for buffer change or subsequent DNA clean-up steps. See Reaction Conditions for FastDigest Enzymes for a table of enzyme activity, heat inactivation and incubation times for this and other FastDigest restriction enzymes. DNA modifying enzymes, such as Klenow Fragment, T4 DNA Ligase, alkaline phosphatases and T4 DNA Polymerase all have 100% activity in FastDigest Buffer. Therefore, enzymes for downstream applications can be directly added to the FastDigest reaction mix.
For additional convenience FastDigest Green Buffer includes a density reagent and two tracking dyes for direct loading of digestion reaction products on gels.
Short incubation times and optimal composition of the universal FastDigest Buffer eliminate star activity effects.
Features
• 100% activity of all FastDigest enzymes in the universal buffer
• 100% buffer compatibility with downstream applications
规格
| Compatible Buffer: | 10x FastDigest Buffer/FastDigest Green Buffer |
|---|---|
| Enzyme: | Eco72I |
| Methylation Sensitivity: | CpG methylation-sensitive, Not dam methylation-sensitive, Not dcm methylation-sensitive |
| Optimal Reaction Temperature: | 37° C |
| Product Line: | FastDigest |
| Product Size: | 200 reactions |
| Sensitive to Heat Inactivation: | Yes |
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文献和实验大鼠热休克蛋白-72(HSP-72)酶联免疫分析(ELISA)
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CD72 分子 CD72 常用单克隆抗体或代号: S- HCL2,J3.109,BU- 40 主要表达细胞: B [B] 分子质量(kDa)和结构: gp43,39(C型凝集素) 功 能: 与CD5结合,B细胞活化、增殖 CD72 Cell surface protein expressed exclusively on B cells; may control B cell
上海西唐生物科技有限公司 021-55229872, 65333639 www.westang.com 人热休克蛋白72 ( HSP72 )ELISA 试剂盒 原理 本实验采用双抗体夹心 ABC-ELISA 法。用抗人 HSP72 单抗包被于酶标板上,标准品和样品中的 HSP72与单抗结合,加入生物素化的抗人 HSP72 ,形成免疫复合物连接在板上,辣根
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