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- 详细信息
- 文献和实验
- 技术资料
- 样本:
Cell Culture Supernates (100 uL), Tissue Lysates (10 uL), Cerebrospinal Fluid (10 uL)
- 标记物:
DAB142
- 适应物种:
人
- 应用:
7.8 - 500 pg/mL
- 检测方法:
Cell Culture Supernates (100 uL), Tissue Lysates (10 uL), Cerebrospinal Fluid (10 uL)
- 检测范围:
7.8 - 500 pg/mL
- 库存:
科研用
- 规格:
96T
阿尔茨海默症(Alzheimer's Disease, AD),俗称老年痴呆症,其特征是在大脑神经细胞中存在老年斑(Senile Plaques,SPs) 和神经元纤维缠结(Neuro Fibrillary Tangles,NFT),老年斑的主要组成蛋白是 β- 淀粉样多肽(Amyloid Peptide,Aβ)40 和 42(43),Aβ42 比 Aβ40 更容易发生聚集。因此,蛋白样多肽的聚集开始于 Aβ42 而不是 Aβ40。出现了 Aβ42 而没有出现 Aβ40 是阿 尔茨海默症的早期特征;出现了 Aβ40,尤其是在成熟老年斑的中心部分出现则代表进入阿尔茨海默症的晚期阶段。
Human Amyloid beta (aa1-42) Quantikine ELISA Kit Summary
Product Summary
(aa1-42) showed linear curves that were parallel to the standard curves obtained using the kit standards. These results indicate that this kit can be used to determine relative mass values for natural human Amyloid beta (aa1-42).
Precision
Cell Culture Supernates, Tissue Lysates, Cerebrospinal Fluid
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 20 | 20 | 20 |
| Mean (pg/mL) | 46.3 | 124 | 264 | 48.9 | 136 | 274 |
| Standard Deviation | 2.46 | 4.4 | 9.38 | 3.48 | 9.02 | 16.1 |
| CV% | 5.3 | 3.5 | 3.6 | 7.1 | 6.6 | 5.9 |
Recovery
The recovery of human Amyloid beta (aa1-42) spiked to levels throughout the range of the assay in various matrices was evaluated.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| Cell Culture Media (n=4) | 97 | 82-114 |
| Cell Lysis Buffer (n=2) | 92 | 90-94 |
| Cerebrospinal Fluid (n=4) | 107 | 95-120 |
Linearity
Data Examples
Human Amyloid beta aa 1-42 ELISA Standard Curve
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【分享】Human press METHODS IN MOLECULAR BIOLOGY
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