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- 详细信息
- 文献和实验
- 技术资料
- 英文名:
Elite ABC-HRP Kit (Peroxidase, Universal) PK-6200
- 供应商:
上海研卉生物
- 库存:
大量
- 规格:
kit
Description
The VECTASTAIN Elite ABC system is the most sensitive avidin/biotin-based peroxidase system and is approximately 5 times more sensitive than the original VECTASTAIN ABC Kit with the same low background. This kit contains a biotinylated universal (anti-mouse/rabbit IgG) secondary antibody.
Features:
- Advanced avidin/biotin technology: The Elite® ABC complex is smaller, very uniform, and highly active, allowing more accessibility for binding to a biotinylated target
- Cost effective: Higher sensitivity leads to lower cost per slide
- Kit contains sufficient reagents to stain approximately 500-1000 sections
Specifications
| Unit Size | 1 kit |
|---|---|
| Applications | Immunohistochemistry / Immunocytochemistry, In situ hybridization, Blotting Applications, Elispot, ELISAs |
| Technology | ABC Elite (Avidin/Biotin) Systems |
| Detection Enzyme(s) | Peroxidase |
| Target Species | Universal (Mouse/Rabbit) |
| Format | Concentrate |
Kit Components
Kit Contents:
- 2 ml Reagent A
- 2 ml Reagent B
- 2 ml biotinylated horse anti-mouse/rabbit IgG
- 3 ml normal horse serum
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文献和实验All-in-One Kit for Immunohistochemical Staining for Tissues with Antibodies
near the tissue sections with a laboratory wipe. Circle each tissue section using the Mini PAP Pen. 2. Proceed immediately to Peroxidase Quenching. Peroxidase Quenching Peroxo-Block™ works efficiently on many tissues, especially
Mapping Protein Distributions on Polytene Chromosomes by Immunostaining
stock solution Nutrient-rich fly medium Phosphate-buffered saline (PBS; pH 7.5) Poly-L-lysine solution (0.1% [w/v] in H2 O; Sigma P8920) Sodium phosphate buffer (10 mM, pH 6.8) Triton X-100 VECTASTAIN Elite ABC Kit
IMMUNOHISTOCHEMISTRY on PARAFFIN OR CRYOSECTIONS
IMMUNOHISTOCHEMISTRY on PARAFFIN OR CRYOSECTIONS USING VECTASTAIN ABC ELITE KIT: 5) Block: a) Block I: 100 mL PBS 1 g Bovine Albumin (Sigma A-9418) PBS + 1% BSA = PBA 1 hr, RT b) Block II: PBA 2% serum from animal used to produce 2° antibody (provided
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