- ￥2570
- PEPROTECH
- 500-P79Bt
- USA
- 2025年07月14日
- WB ELISA
- 兔
- 人
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
500-P79Bt
- 靶点：
Biotinylated Anti-Rat CNTF-生物素标记CNTF
- 级别：
科研
- 目录编号：
500-P79Bt
- 克隆性：
多克隆
- 抗原来源：
Recombinant Rat CNTF (PeproTech catalog# 450-50)
- 抗体英文名：
Biotinylated Anti-Rat CNTF-生物素标记CNTF
- 抗体名：
Biotinylated Anti-Rat CNTF-生物素标记CNTF
- 标记物：
Biotinylated
- 宿主：
兔
- 适应物种：
人
- 应用范围：
WB ELISA
- 浓度：
Biotinylated Anti-Rat CNTF-生物素标记CNTF
- 规格：
25ug
产品详情
Source: Polyclonal Rabbit
Preparation: Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant Rat CNTF. Anti-Rat CNTF specific antibody was purified by affinity chromatography and then biotinylated.
Immunogen: E.coli derived Recombinant Rat CNTF (PeproTech catalog# 450-50)
Sandwich ELISA: To detect Rat CNTF by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.25 – 1.0 μg/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with PeproTech’s Polyclonal Anti-Rat CNTF (500-P79) as a capture antibody, allows the detection of at least 0.2 – 0.4 ng/well of recombinant Rat CNTF.
Western Blot: To detect Rat CNTF by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant Rat CNTF is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
| 500-P79-50 | Rabbit Anti-Rat CNTF | 50ug |
| 500-P79-100 | Rabbit Anti-Rat CNTF | 100ug |
| 500-P79-1000 | Rabbit Anti-Rat CNTF | 1000ug |
| 500-P79Bt-25 | Biotinylated Rabbit Anti-Rat CNTF | 25ug |
| 500-P79Bt-50 | Biotinylated Rabbit Anti-Rat CNTF | 50ug |
| 500-P79Bt-1000 | Biotinylated Rabbit Anti-Rat CNTF | 1000ug |
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文献和实验在板上，辣根过氧化物酶标记的 Streptavidin 与生物素结合，加入底物工作液显蓝色，最后加终止液硫酸，在 450nm 处测 OD 值， CNTF 浓度与 OD 值成正比，可通过绘制标准曲线求出标本中 CNTF 浓度。 试剂盒组成 （ 2-8℃ 保存） 酶标 板（ Coated Wells ） 96 孔 酶标抗体工作液（ Enzyme
化的抗小鼠 CNTF ，形成免疫复合物连接在板上，辣根过氧化物酶标记的 Streptavidin 与生物素结合，加入底物工作液显蓝色，最后加终止液硫酸，在 450nm 处测 OD 值，CNTF 浓度与 OD 值成正比，可通过绘制标准曲线求出标本中CNTF 浓度。 试剂盒组成 （ 2-8 ℃保存） 酶标 板 （Coated Wells ） 96 孔
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