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- 详细信息
- 文献和实验
- 技术资料
- 英文名：
FastDigest BamHI
- 供应商：
上海研卉生物科技有限公司
- 库存：
大量
- 规格：
800 reactions
描述
| 5' | G ↓ | G | A | T | C | C | 3' | ||||
| 3' | C | C | T | A | G ↑ | G | 5' |
Thermo Scientific FastDigest BamHI restriction enzyme recognizes G^GATCC site and cuts best at 37°C in 5–15 minutes using universal FastDigest Buffer.
Thermo Scientific FastDigest BamHI is one of an advanced line of fast restriction enzymes that are all 100% active in the universal FastDigest and FastDigest Green reaction buffers.
The universal buffer allows rapid single-, double-, or multiple DNA digestion within 5–15 minutes eliminating any need for buffer change or subsequent DNA clean-up steps. See Reaction Conditions for FastDigest Enzymes for a table of enzyme activity, heat inactivation and incubation times for this and other FastDigest restriction enzymes. DNA modifying enzymes, such as Klenow Fragment, T4 DNA Ligase, alkaline phosphatases and T4 DNA Polymerase all have 100% activity in FastDigest Buffer. Therefore, enzymes for downstream applications can be directly added to the FastDigest reaction mix.
For additional convenience FastDigest Green Buffer includes a density reagent and two tracking dyes for direct loading of digestion reaction products on gels.
Short incubation times and optimal composition of the universal FastDigest Buffer eliminate star activity effects.
Features
• 100% activity of all FastDigest enzymes in the universal buffer
• 100% buffer compatibility with downstream applications
• Complete digestion in 5–15 minutes
规格
| Compatible Buffer: | 10x FastDigest Buffer/FastDigest Green Buffer |
|---|---|
| Enzyme: | BamH I |
| Methylation Sensitivity: | Not CpG methylation-sensitive, Not dam methylation-sensitive, Not dcm methylation-sensitive |
| Optimal Reaction Temperature: | 37° C |
| Product Line: | FastDigest |
| Product Size: | 800 reactions |
| Sensitive to Heat Inactivation: | Yes |
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文献和实验相关专题 重组DNA技术工具酶 限制性核酸内切酶产品选择专题 实验目的 1. 学会DNA限制性内切酶 酶切技术。 2. 琼脂 糖凝胶电泳法观察酶切DNA的结果。 实验原理 质粒pUC118上有限制性内切酶BamHI的识别序列G↓GATCC，用BamHI酶切后形成线性质粒DNA。琼脂 糖凝胶电泳可以按酶切产物分子量的大小分离DNA片段，小片段比大片段迁移快，凝胶上迁移的距离
Methylation analyis using restriction enzyme digestion
. Therefore first of all define the region of interest flanked with restriction sites for CG methylation insensitive enzymes (BamHI for example), and containing not more than 5-6 sites for HpaII. The probe used for Southern blot hybridisation should be located within this region
cindyly267 最近在构建一个克隆，两个多月了都还没成功。具体情况如下：使用的是promega公司的Halotag载体，约3900kb。片段约500kb。酶切位点是SgfI 和 BamHI。之前用别人连了片段的载体酶切后再做载体的，无论怎么切都是自连。这次使用了公司的原始载体（带致死基因），片段也是从T载上切下来的，所以感觉载体和片段都切的没问题了，结果一个都没长（可能有自连的但是死了）。 引物，酶切位点什么的都检查过了没问题，实在是没
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