- ￥425
- Sigma
- V900312
- Sigma
- 2025年08月26日
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- 技术资料
- 英文名：
Trizma® hydrochloride
- CAS号：
1185-53-1
- 供应商：
1185-53-1
- 库存：
V900312
- 规格：
500g
Application
The pH values of all buffers are temperature and concentration dependent. For Tris buffers, pH increases about 0.03 unit per °C decrease in temperature, and decreases 0.03-0.05 unit per ten-fold dilution.
For precise applications, use a carefully calibrated pH meter with a glass/calomel combination electrode.
grade Vetec™ reagent grade
assay ≥99%
useful pH range 7.0 - 9.0
pKa (25 °C) 8.1
应用
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文献和实验How to make DEPC-treated water and Tris Buffer
Add 0.1 ml DEPC to 100 ml of the solution to be treated and shake vigorously to bring the DEPC into solution.Let the solution incubate for 12 hours at 37℃.Autoclave for 15 minutes to remove any trace of DEPC.Notes:How to make DEPC-treated Tris
Role of TRIS in DNA extraction-Molecular Biology
i want to know why exactly tris is used as a buffer when we can use any inorganic salt also to reach pH 8 so what is the function of tris? -------------------------------------------------------------------------------- The pH
一、TBS的配制 0.05 M TBS ( pH7.4 ) 的配制： Tris ( 三羟甲基胺基甲烷 ) 12.1g NaCl 17.5g 加蒸馏水 1500ml 磁性搅拌下滴加浓HCl至pH为7.4，再加蒸馏水至2000ml，即可。 如需含1% Triton X-100，则在滴加HCl前先加入20
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