Mouse BDNF ELISA Kit (Sandwich

Mouse BDNF ELISA Kit (Sandwich ELISA) - LS-F24714

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LS-F24714-1
1 plate
$690 USD
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Popular BDNF Elisa Kits

Human BDNF ELISA Kit (Sandwich ELISA) - LS-F35
Type: Quantitative Sandwich ELISA
Format: 96-Well Microplate
Reactivity: Human
Range: 0.066-16 ng/ml
Detection: Colorimetric - 450nm (TMB)
Sample Types: Cell Culture Supernatants, Serum, Plasma

Human BDNF ELISA Kit (Sandwich ELISA) - LS-F2402
Type: Quantitative Sandwich ELISA
Format: 96-Well Microplate
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Mouse BDNF ELISA Kit (Sandwich ELISA) - LS-F2404
Type: Quantitative Sandwich ELISA
Format: 96-Well Microplate
Reactivity: Mouse
Range: 31.2-2000 pg/ml
Detection: Colorimetric - 450nm (TMB)
Sample Types: Cell Culture Supernatants, Cell Lysates, Serum, Plasma

Human BDNF ELISA Kit (Sandwich ELISA) - LS-F3960
Type: Sandwich ELISA
Format: 96-Well Strip Plate
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Range: 0.156-10 ng/ml
Detection: Colorimetric - 450nm (TMB)
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Type: Sandwich ELISA
Format: 96-Well Strip Plate
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Sample Types: Cell Culture Supernatants, Cell Lysates, Serum, Tissue Homogenates, Plasma

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Product Description

LS-F24714 is a 96-well enzyme-linked immunosorbent assay (ELISA) for the Quantitative detection of Mouse BDNF in samples of Cell Culture Supernatants, Plasma and Serum. It is based upon a Sandwich assay principle and can be used to detect levels of BDNF as low as 2 picograms per milliliter.
About BDNF
During development, promotes the survival and differentiation of selected neuronal populations of the peripheral and central nervous systems. Participates in axonal growth, pathfinding and in the modulation of dendritic growth and morphology. Major regulator of synaptic transmission and plasticity at adult synapses in many regions of the CNS. Uniprot: P23560 NCBI:NM_001709 NP_001700.2

Synonyms: BDNF ElisaKit, ANON2 ElisaKit, Abrineurin ElisaKit, BULN2 ElisaKit, Neurotrophin ElisaKit

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Specifications

Type
 
Sandwich ELISA (enzyme-linked immunosorbent assay) kit
Target
 
BDNF
Reactivity
 
Mouse
Specificity
 
For the detection and quantification of endogenous levels of natural and/or recombinant Mouse BDNF proteins.
Manual
 

Intended Sample Types
 
Cell Culture Supernatants, Plasma, Serum
Format
 
96-Well Strip Plate
Detection
 
Colorimetric - 450nm (TMB)
Measurement
 
Quantitative
Detection Range
 
31.2 - 2000 pg/ml
Sensitivity
 
2 pg/ml
Storage
 
Short term: 4°C; Long term: see manual.
Quality Assurance
 
Due to their limited shelf life, LSBio ELISA kits are not typically stocked as finished goods. Upon receipt of an order each kit is assembled and tested to ensure that it meets specifications before shipping. Minor changes may occur to the Range, Sensitivity, and Precision. In the event of a significant change the order would be confirmed with the customer before shipping ELISA kit lot numbers reflect the date of final assembly and testing for each specific kit rather than a bulk manufactured lot. All kits are tested to confirm that they fall within their defined Inter- and Intra- assay coefficient of variation.
Kit Components
 

• Coated 96-well Strip Plate
• Standard (Lyophilized)
• Biotinylated Detection Antibody (100x)
• Avidin-Biotin-HRP Complex (100x)
• Sample Diluent
• Biotinylated Detection Antibody Diluent
• Avidin-Biotin-HRP Complex Diluent
• Wash Buffer (20x)
• TMB Substrate
• Stop Solution
• Adhesive Plate Sealers

Background
 
Brain-derived neurotrophic factor (BDNF) is a prosurvival factor induced by cortical neurons that is necessary for survival of striatal neurons in the brain. It is a secreted protein with the molecular weight of 27.8kDa, consisting of 247 amino acids. It is known to promote neuronal survival and differentiation. BDNF shares substantial amino acid sequence identity with nerve growth factor (NGF). BDNF and neurotrophin-3 (NT-3) are two recently cloned neurotrophic factors that are homologous to NGF. mRNA products of the BDNF and NT-3 genes are detected in the adult human brain, suggesting that these proteins are involved in the maintenance of the adult nervous system. BDNF and other neurotrophins are critically involved in long-term potentiation (LTP). BDNF-mediated LTP is induced postsynaptically. BDNF has trophic effects on serotonergic (5-HT) neurons in the central nervous system. BDNF has an essential maintenance function in the regulation of anxiety-related behavior and in food intake through central mediators in both the basal and fasted state. It plays a role in treating breathing disorders such as respiratory insufficiency after spinal injury. The mature form of BDNF is identical in all mammals examined, and the gene encoding human BDNF to chromosome 11, band p13.
Restrictions
 
For research use only.
Guarantee
 
This elisa kit carries the LSBio 100% Guarantee.
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