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- 详细信息
- 询价记录
- 技术资料
- 英文名：
Cell Navigator™ 溶酶体染色试剂盒 *红色荧光*
- 保存条件：
低温
- 规格：
500 Tests
Cell Navigator™ 溶酶体染色试剂盒 *红色荧光*
| Catalog | Unit Size | Price(USD) | Additional Information |
| 22655 | 500 Tests | ¥2219 | Blue Fluorescence |
| 22659 | 500 Tests | ¥2219 | Deep Red Fluorescence |
| 22656 | 500 Tests | ¥2219 | Green Fluorescence |
| 22651 | 500 Tests | ¥2973 | Green Fluorescence with 405 nm Excitation |
| 22652 | 500 Tests | ¥2973 | NIR Fluorescence |
| 22657 | 500 Tests | ¥2219 | Orange Fluorescence |
| 22658 | 500 Tests | ¥2219 | Red Fluorescence |
Lysosomes are cellular organelles which contain acid hydrolase enzymes to break up waste materials and cellular debris. Lysosomes digest excess or worn-out organelles, food particles, and engulfed viruses or bacteria. The membrane around a lysosome allows the digestive enzymes to work at pH 4.5. The interior of the lysosomes is acidic (pH 4.5-4.8) compared to the slightly alkaline cytosol (pH 7.2). The lysosome maintains this pH differential by pumping protons from the cytosol across the membrane via proton pumps and chloride ion channels. Our Cell Navigator™ fluorescence imaging kits are a set of fluorescence imaging tools for labeling sub-cellular organelles such as membranes, lysosomes, mitochondria, nuclei, etc. The selective labeling of live cell compartments provides a powerful method for studying cellular events in a spatial and temporal context. This particular kit is designed to label lysosomes of live cells in orange fluorescence. LysoBrite™ Red, the proprietary lysotropic dye used in the kit, selectively accumulates in lysosomes probably via the lysosome pH gradient. The lysotropic indicator is a hydrophobic compound that easily permeates intact live cells, and trapped in lysosomes after it gets into cells. Its fluorescence is significantly enhanced upon entering lysosomes. This key feature significantly reduces its staining background and makes it useful for a variety of studies, including cell adhesion, chemotaxis, multidrug resistance, cell viability, apoptosis and cytotoxicity. It is suitable for proliferating and non-proliferating cells, and can be used for both suspension and adherent cells. LysoBrite™ dyes significantly outperform the equivalent LysoTracker ™dyes (from Invitrogen). LysoBrite™ dyes can stay in live cells for more than a week with very minimal cell toxicity while the LysoTracker dyes can only be used for a few hours. LysoBrite™ dyes can survive a few generations of cell division. In addition, LysoBrite™ dyes are much more photostable than the LysoTracker dyes.
Platform
| Fluorescence microscope | |
| Excitation | TRITC filter |
| Emission | TRITC filter |
| Recommended plate | Black wall/clear bottom |
Components
| Component A: LysoBrite™ Red | 1 vial (100 µL, 500X DMSO stock solution) |
| Component B: Live Cell Staining Buffer | 1 bottle (50 mL) |
Example protocol
At a glance
Protocol summary
- Prepare cells
- Add LysoBrite™ Red working solution
- Incubate at 37°C for 30 minutes
- Wash the cells
- Analyze the cells under fluorescence microscope at Ex/Em = 575/600 nm (TRITC filter set)
Important notes
Thaw all the kit components at room temperature before starting the experiment.
Preparation of cell samples
For guidelines on cell sample preparation, please visit https://www.aatbio.com/resources/guides/cell-sample-preparation.html
Preparation of working solution
Add 20 µL of 500X LysoBrite™ Red (Component A) to 10 mL of Live Cell Staining Buffer (Component B) to make LysoBrite™ Red working solution. Protect from light. Note: 20 µL of 500X LysoBrite™ Red (Component A) is enough for one 96-well plate. The optimal concentration of the fluorescent lysosome indicator varies depending on the specific application. The staining conditions may be modified according to the particular cell type and the permeability of the cells or tissues to the probe.
Procedure
For adherent cells:
- Grow cells either in a 96-well black wall/clear bottom plate (100 µL/well/96-well plate) or on cover-slips inside a petri dish filled with the appropriate culture medium.
- When cells reach the desired confluence, add equal volume of LysoBrite™ Red working solution.
- Incubate the cells in a 37°C, 5% CO2 incubator for 30 minutes.
- Wash the cells twice with pre-warmed (37°C) Hanks and 20 mM Hepes buffer (HBSS) or buffer of your choice, fill the cell wells with HBSS or growth medium.
- Observe the cells using a fluorescence microscope with TRITC filter set (Ex/Em = 575/600 nm). Note: It is recommended to increase either the labeling concentration or the incubation time to allow the dye to accumulate if the cells do not appear to be sufficiently stained.
For suspension cells:
- Add equal volume of LysoBrite™ Red working solution into the cells.
- Incubate the cells in a 37°C, 5% CO2 incubator for 30 minutes.
- Wash the cells twice with pre-warmed (37°C) Hanks and 20 mM Hepes buffer (HBSS) or buffer of your choice, fill the cell wells with HBSS or growth medium.
- Observe the cells using a fluorescence microscope with TRITC filter set (Ex/Em = 575/600 nm). Note: It is recommended to increase either the labeling concentration or the incubation time to allow the dye to accumulate if the cells do not appear to be sufficiently stained. Suspension cells may be attached to cover-slips that have been treated with BD Cell-Tak® (BD Biosciences) and stained as adherent cells.
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溶酶体染色试剂盒
¥2219
