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- 详细信息
- 文献和实验
- 技术资料
- 英文名:
RBC Lysis Buffer (10X)
- 保存条件:
低温
- 规格:
100 ml
Description
Red Blood Cell (RBC) Lysis Buffer has been designed, formulated, and tested to ensure optimal lysis of RBCs in single cell suspensions with minimal effects on leukocytes. RBC Lysis Buffer is supplied as a 10X solution containing ammonium chloride, potassium carbonate, and EDTA, and should be diluted in deionized water prior to use. Nucleated RBCs are not effectively lysed with ammonium chloride.
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文献和实验10X TBE BUFFER 3 Liters 324 g Tris base 165g Boric acid 120mls 0.5M EDTA (pH 8.0) autoclave for 20 min 2 Liters 216g Tris base 110g Boric acid 80mls 0.5M EDTA (pH 8.0) autoclave
ChIP Protocol-Mechanical Breakage & FA Lysis Buffer
ChIP Protocol-Mechanical Breakage & FA Lysis Buffer Day -2: o Start a 5ml overnight culture from a single colony. Day -1: o Start a larger overnight culture using part of the 5ml culture (subculture). Day
Jacobs:Protocol Total Protein Isolation Using RIPA Lysis Buffer
Procedure Each group will receive one tissue culture dish containing 3T3 fibroblasts Label 2 microcentrifuge tubes: Protein Group# One person will prepare RIPA Lysis buffer for the entire class: Add 10 μ
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