产品封面图

BD MitoScreen (JC-1),细胞凋亡检测

收藏
  • ¥6907
  • bd
  • 551302
  • USA
  • 2025年10月03日
    avatar
  • 企业认证

    点击 QQ 联系

    • 详细信息
    • 文献和实验
    • 技术资料
    • 英文名

      BD MitoScreen (JC-1)

    • 保存条件

      低温

    • 规格

      100 Tests

    BD MitoScreen (JC-1),细胞凋亡检测
    Reactivity
    Human, Mouse (QC Testing)

    Description

    Components
    Jc-1: 4 Amber Vials, lyophilized
    Assay Buffer (10x), 60 mL

    Preparation and Storage

    Store the unopened vials of JC-1 and 10× Assay Buffer at 2° – 8°C.

    1. Barbieri, D., M.P. Abbracchio, S. Salvioli, D. Monti, A. Cossarizza, S. Ceruti, R. Brambilla, F. Cattabeni, K.A. Jacobson, and C. Franceschi. 1998. Apoptosis by 2-chloro-2’-deoxy-adenosine and 2-chloro-adenosine in human peripheral blood mononuclear cells. Neurochemi. Int. 32:493-504.

    2. Cossarizza, A., M. Baccarani-Contri, G. Kalashnikova, and C. Franceschi. 1993. A new method for the cytofluorimetric analysis of mitochondrial membrane potential using the J-aggregate forming lipophilic cationic 5,5’,6,6’-tetrachloro-1,1’,3,3’-tetraethylbenzimidazolcarbocyanine iodide (JC-1). Biochem. Biophs. Res. Comm. 197:40-45.

    3. Facompre, M., N. Wattez, J. Kluza, A. Lansiaux, and C. Bailly. 2000. Relationship between cell cycle changes and variations of the mitochondrial membrane potential induced by etoposide. Mol. Cell Biol. Res. Comm. 4:37-42.

    4. Gravance, C.G., D.L. Garner, J. Baumber, and B.A.Ball. 2000. Assessment of equine sperm mitochondrial function using JC-1. Theriogenology 53:1691-1703

    5. Mantymaa, P., T. Sitonen, T. Guttorm, M. Saily, V. Kinnula, E-R. Savolainen, and P. Koistinen. 2000. Introduction of mitochondrial manganes superoxide dismutase confers resistance to apoptosis in acute myeloblastic leukemia cells exposed to etoposide. British J. Haematol. 108:574-581.

    6. Mathur, A., Y. Hong, B.K. Kemp, A.A. Barrientos, and J.D. Erusalimsky. 2000. Evaluation of fluorescent dyes for the detection of mitochondrial membrane potential changes in cultured cardiomyocytes. J. Cardiovascular Res. 46:126-138.

    7. Petit, P.X., H. Lecoeur, E. Zorn, C. Dauguet, B. Mignotte, and M-L. Gougeon. 1995. Alterations in mitochondrial structure and function are early events of dexamethasone-induced thymocyte apoptosis. J. Cell Biol. 130:157-167.

    8. Salvioli, S., Ardizzoni, A., Franceschi, and A. Cossarizza 1997. JC-1, but not DiOC6(3) orrhodamine 123, is a reliable fluorescent probe to assess ∆ψ changes in intact cells: implications for studies on mitochondrial functionality during apoptosis. FEBS 411:72-82.

    9. Salvioli, S.,k J. Dobrucki, L. Moretti, L. Troiano, M.G. Fernandez, M. Pinti, J. Pedrazzi, C. Franceshi, and A. Coassarizza. 2000. Mitochondrial heterogeneity during staurosporine-induced apoptosis in HL60 cells: Analysis at the single cell and single organelle level. Cytometry 40:189-197.

    The BD™ MitoScreen Kit is designed for use in flow cytometry. It consists of two reagents, JC-1 and 10× Assay Buffer. JC-1 excites at 488 – 490 nm. The monomeric form emits at 527 nm; J-aggregates emit at 590 nm. There are enough reagents to test 100 samples by flow cytometry using the protocol provided.

    风险提示:丁香通仅作为第三方平台,为商家信息发布提供平台空间。用户咨询产品时请注意保护个人信息及财产安全,合理判断,谨慎选购商品,商家和用户对交易行为负责。对于医疗器械类产品,请先查证核实企业经营资质和医疗器械产品注册证情况。

    图标文献和实验
    相关实验
    • 【求助】JC-1检测

      yy59410 请问,我现在要测线粒体膜通透性改变即JC-1的检测,想问下国产的试剂盒里凯基的好还是碧云天的好。希望高手指路啊。 freecell 效果差不多。 yy59410 谢谢! 还有一个就是我用JC-1测MMP,是不是没必要再用罗丹明测ROS了! 我做的是凋亡检测! wudonghui3516010 我现在也是在用JC-1,效果

    • 常用细胞凋亡检测方法(图)

      ,而线粒体跨膜电位DYmt的下降,被认为是细胞凋亡级联反应过程中最早发生的事件,它发生在细胞核凋亡特征(染色质浓缩、DNA断裂)出现之前,一旦线粒体DYmt崩溃,则细胞凋亡不可逆转。 线粒体跨膜电位的存在,使一些亲脂性阳离子荧光染料如Rhodamine 123、3,3-Dihexyloxacarbocyanine iodide[DiOC6(3)]、Tetrechloro-tetraethylbenzimidazol carbocyanine iodide[JC-1]、Tetramethyl

    • 常用细胞凋亡检测方法(三)

      -tetraethylbenzimidazol carbocyanine iodide[JC-1]、Tetramethyl rhodamine methyl ester(TMRM)等可结合到线粒体基质,其荧光的增强或减弱说明线粒体内膜电负性的增高或降低. 方法: 将正常培养的细胞和诱导凋亡的细胞加入使用终浓度为Rhodamine 123(1mM)或终浓度为DiOC6(25nM),JC-1(1mM), TMRM(100nM), 37°C平衡30min, 流式细胞计检测细胞的荧光强度. 注意事项 1. 始终保持平衡染液

    图标技术资料

    暂无技术资料 索取技术资料

    同类产品报价

    产品名称
    产品价格
    公司名称
    报价日期
    ¥2200
    上海研卉生物科技有限公司
    2025年12月04日询价
    ¥600
    上海贝博生物科技有限公司
    2025年07月16日询价
    ¥120
    翌圣生物科技(上海)股份有限公司
    2025年12月25日询价
    询价
    上海莼试生物技术有限公司
    2025年07月16日询价
    ¥2000
    上海伟进生物科技有限公司
    2025年12月25日询价
    BD MitoScreen (JC-1),细胞凋亡检测
    ¥6907