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- ACRO
- HSA-H5220
- 2025年07月16日
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- 详细信息
- 询价记录
- 技术资料
- 供应商：
上海研卉生物科技有限公司
- 库存：
大量
- 英文名：
Human Serum Albumin Protein, His Tag (MALS verified)
- 规格：
500ug
Human Serum Albumin Protein, His Tag (MALS verified)
分子别名（Synonym）
ALB,Alb,Alb-1,Alb1,Serum albumin,FCGRT,HAS,MSA,RSA,CSA
表达区间及表达系统（Source）
Human Serum Albumin, His Tag (HSA-H5220) is expressed from human 293 cells (HEK293). It contains AA Asp 25 - Leu 609 (Accession # P02768-1).
Predicted N-terminus: Asp 25
Request for sequence
蛋白结构（Molecular Characterization）
This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 67.4 kDa. The protein migrates as 66 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
内毒素（Endotoxin）
Less than 1.0 EU per μg by the LAL method.
纯度（Purity）
>95% as determined by SDS-PAGE.
>90% as determined by SEC-MALS.
制剂（Formulation）
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.
Contact us for customized product form or formulation.
重构方法（Reconstitution）
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
分子别名（Synonym）
ALB,Alb,Alb-1,Alb1,Serum albumin,FCGRT,HAS,MSA,RSA,CSA
表达区间及表达系统（Source）
Human Serum Albumin, His Tag (HSA-H5220) is expressed from human 293 cells (HEK293). It contains AA Asp 25 - Leu 609 (Accession # P02768-1).
Predicted N-terminus: Asp 25
Request for sequence
蛋白结构（Molecular Characterization）
This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 67.4 kDa. The protein migrates as 66 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
内毒素（Endotoxin）
Less than 1.0 EU per μg by the LAL method.
纯度（Purity）
>95% as determined by SDS-PAGE.
>90% as determined by SEC-MALS.
制剂（Formulation）
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.
Contact us for customized product form or formulation.
重构方法（Reconstitution）
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
存储（Storage）
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
电泳（SDS-PAGE）
Human Serum Albumin, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.
SEC-MALS
The purity of Human Serum Albumin, His Tag (Cat. No. HSA-H5220) was more than 90% and the molecular weight of this protein is around 60-75 kDa verified by SEC-MALS.
Report
活性（Bioactivity）-SPR
Human Serum Albumin Protein, His Tag (Cat. No. HSA-H5220) immobilized on CM4 Chip can bind Human FCGRT&B2M Heterodimer Protein, His Tag (Cat. No. FCN-H52W7) with an affinity constant of 0.381 μM as determined in a SPR assay (Biacore T200) (Routinely tested).
Protocol
活性（Bioactivity）-BLI
Loaded Biotinylated Human FCGRT&B2M Heterodimer Protein, His,Avitag (Cat. No. FCM-H82W7) on SA Biosensor, can bind Human Serum Albumin, His Tag (Cat. No. HSA-H5220) with an affinity constant of 0.647 μM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
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