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- HSA-H5220
- 2025年07月16日
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- 详细信息
- 询价记录
- 文献和实验
- 技术资料
- 供应商：
上海研卉生物科技有限公司
- 库存：
大量
- 英文名：
Human Serum Albumin Protein, His Tag (MALS verified)
- 规格：
500ug
Human Serum Albumin Protein, His Tag (MALS verified)
分子别名（Synonym）
ALB,Alb,Alb-1,Alb1,Serum albumin,FCGRT,HAS,MSA,RSA,CSA
表达区间及表达系统（Source）
Human Serum Albumin, His Tag (HSA-H5220) is expressed from human 293 cells (HEK293). It contains AA Asp 25 - Leu 609 (Accession # P02768-1).
Predicted N-terminus: Asp 25
Request for sequence
蛋白结构（Molecular Characterization）
This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 67.4 kDa. The protein migrates as 66 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
内毒素（Endotoxin）
Less than 1.0 EU per μg by the LAL method.
纯度（Purity）
>95% as determined by SDS-PAGE.
>90% as determined by SEC-MALS.
制剂（Formulation）
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.
Contact us for customized product form or formulation.
重构方法（Reconstitution）
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
分子别名（Synonym）
ALB,Alb,Alb-1,Alb1,Serum albumin,FCGRT,HAS,MSA,RSA,CSA
表达区间及表达系统（Source）
Human Serum Albumin, His Tag (HSA-H5220) is expressed from human 293 cells (HEK293). It contains AA Asp 25 - Leu 609 (Accession # P02768-1).
Predicted N-terminus: Asp 25
Request for sequence
蛋白结构（Molecular Characterization）
This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 67.4 kDa. The protein migrates as 66 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
内毒素（Endotoxin）
Less than 1.0 EU per μg by the LAL method.
纯度（Purity）
>95% as determined by SDS-PAGE.
>90% as determined by SEC-MALS.
制剂（Formulation）
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.
Contact us for customized product form or formulation.
重构方法（Reconstitution）
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
存储（Storage）
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
电泳（SDS-PAGE）
Human Serum Albumin, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.
SEC-MALS
The purity of Human Serum Albumin, His Tag (Cat. No. HSA-H5220) was more than 90% and the molecular weight of this protein is around 60-75 kDa verified by SEC-MALS.
Report
活性（Bioactivity）-SPR
Human Serum Albumin Protein, His Tag (Cat. No. HSA-H5220) immobilized on CM4 Chip can bind Human FCGRT&B2M Heterodimer Protein, His Tag (Cat. No. FCN-H52W7) with an affinity constant of 0.381 μM as determined in a SPR assay (Biacore T200) (Routinely tested).
Protocol
活性（Bioactivity）-BLI
Loaded Biotinylated Human FCGRT&B2M Heterodimer Protein, His,Avitag (Cat. No. FCM-H82W7) on SA Biosensor, can bind Human Serum Albumin, His Tag (Cat. No. HSA-H5220) with an affinity constant of 0.647 μM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
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文献和实验丰富，可构建多种类型的噬菌体免疫库和天然库（scFv、Fab、VHH等）。 2. 筛选：普健自有噬菌体天然抗体文库，序列正确性高，来源丰富，库容大。 3. 筛选通量高，已成功交付多例建库及筛选项目，客户分布于欧洲及亚洲。 4. 载体带有His标签，可直接用于表达抗体，方便抗体纯化验证。 5. 现已成功开发出兔scFv天然噬菌体文库产品：ATA-Rabbit Naïve ScFv I（库容：1.09×1010 pfu）。 经兔scFv天然库筛选出的序列，拼接成全长IgG重组
有一钙质的裂缝。 2．进化程度高，接近于人类。因此，它们具有与人相近似的生理生化代谢特性和相同的药物代谢酶。大量实验证明，灵长目动物在药物代谢方式等上远较非灵长目动物更接近于人，在灵长目动物中，从进化尺度上越是接近于人，其代谢方式也越和人近似，见表2-3和表2-4。 表2-3 灵长目动物的进化谱* 类人猿亚目 1．人 2．猩猩科（如黑猩猩和大猩猩） 3．猴科或旧域猴（如猕猴和狒狒） 4．新域猴（如代帽猿、松鼠猴、狨） 原猴亚目 5．狐猴科（如狐猴、懒猴
代谢酶。大量实验证明，灵长目动物在药物代谢方式等上远较非灵长目动物更接近于人，在灵长目动物中，从进化尺度上越是接近于人，其代谢方式也越和人近似，见表2-3和表2-4。 表2-3 灵长目动物的进化谱* 类人猿亚目 1．人 2．猩猩科（如黑猩猩和大猩猩） 3．猴科或旧域猴（如猕猴和狒狒） 4．新域猴（如代帽猿、松鼠猴、狨
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