- ￥1066
- BD
- 354351
- USA
- 2025年07月11日
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- 详细信息
- 询价记录
- 文献和实验
- 技术资料
- 库存：
1
- 供应商：
BD
- 英文名：
ITS Premix
- 规格：
5ml
ITS Premix Universal Culture Supplement, 5mL, 1/Pack (Product #354351)
Corning® ITS Premix Universal Culture Supplement, 5mL, contains insulin, human transferrin, and selenous acid, the three most universally essential components of defined culture media. The concentrated supplement stimulates cell proliferation of a variety of cells under serum-reduced conditions. 5mL (5L equivalent), 1/pack
The ITS Premix (354350 and 354231) contains the following when used at the recommended dilution: Insulin (5ug/mL), Transferrin (5ug/mL), and Selenious Acid (5ng/mL)
Formulation
Corning ITS Premix -Lyophilized from a water solution; reconstitute in 20 mL (cat. no. 354350) or 5 mL (cat. no. 354351) dH2O (stock solution).
Quality
• Tested for ability to promote proliferation of HeLa cells in serum-free medium
• Filtered (0.2 µm membrane) and tested and found negative for bacteria, fungi and mycoplasma
Preparation and Storage
Corning ITS Premix: Stable for at least three months at 2° to 8°C (lyophilized) or = three months at -20°C (solubilized). Avoid multiple freeze/thaws.
Tip
Corning ITS Premix - Recommended dilution - 1:1000 (dilute stock solution in serum-free medium
国产替代品
胰岛素-转铁蛋白-硒-乙chun胺添加剂（ITS-X），100X
货号：S452J7
规格：10 mL风险提示：丁香通仅作为第三方平台，为商家信息发布提供平台空间。用户咨询产品时请注意保护个人信息及财产安全，合理判断，谨慎选购商品，商家和用户对交易行为负责。对于医疗器械类产品，请先查证核实企业经营资质和医疗器械产品注册证情况。
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文献和实验N2 supplement的配置和N2 Supplement操作手册
of 100 ml medium add 1 ml N2 Supplement (100x) into 99 ml of the appropriate base medium. Cell culture vessels must be coated with poly-D-lysine (0.05 mg/ml). Fibronectin must be added at a final concentration of 5-10 μg/ml directly
, they are transferred to the "tissue culture" incubator. C . Using sterile forceps, transfer tissue to the petri dish, a 2mm cubed sample is sufficient. If the sample is large, cut off a section. Add 2.0ml of media to the dish. Using scissors, cut tissue into small
1. Medium preparation 1) Base Medium consists of 99mL of Williams’ Medium E combined with 1 mL of GlutaMAX™ I 2) Thaw the HepaRG™ Supplement by placing the bottle in a 37°C water bath until completely thawed. 3) Prepare the HepaRG
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