- Catalog# / Size
- 362603 / 100 tests
- Clone
- J095G46 (See other available formats)
- Other Names
- Hexa histidine-tag, 6xHis-tag, 6XHis, 6His
- Ave. Rating
This antibody was raised against linear 8XHis tag. It reacts with His tag protein, either C, N, or internal His6 and up repeats. It can be used for Western blotting to detect linear His repeat (His6 or more). It can also be used for ICFC and ICC staining of His-Tagged protein.
- Catalog# / Size
- 362603 / 100 tests
- Clone
- J095G46 (See other available formats)
- Other Names
- Hexa histidine-tag, 6xHis-tag, 6XHis, 6His
- Ave. Rating
- 0 reviews
This antibody was raised against linear 8XHis tag. It reacts with His tag protein, either C, N, or internal His6 and up repeats. It can be used for Western blotting to detect linear His repeat (His6 or more). It can also be used for ICFC and ICC staining of His-Tagged protein.
Product Details
- Reactivity
- Human, Mouse, Rat, All species
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% (w/v) BSA (origin USA).
- Preparation
- The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions. The solution is free of unconjugated PE and unconjugated antibody.
- Concentration
- Lot-specific (please contact technical support for concentration and total µg amount, or use our Lookup tool if you have a lot number.)
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
- Recommended Usage
-
Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells or 5 µl per 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
- Excitation Laser
-
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm) - Application Notes
-
This antibody was raised against linear 8XHis tag. It reacts with proteins displaying repeats of 6 Histidines (His6) or more in either internal or C and N terminal regions. It is suitable for ICFC.
Clone J095G46 has been validated for immunocytochemistry (ICC). - RRID
- AB_2563634 (BioLegend Cat. No. 362603)
Other Formats
APC anti-His Tag, Alexa Fluor® 594 anti-His Tag, Purified anti-His Tag, Alexa Fluor® 647 anti-His Tag, Direct-Blot™ HRP anti-His Tag, Alexa Fluor® 488 anti-His TagProduct Data
