- ¥8400
- GE
- 17508701
- USA
- 2025年11月16日
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53 mL
| 17508701 | HiPrep 26/10 Desalting | 53 mL |
HiPrep 26/10 Desalting are convenient, easy-to-use, 53 mL columns prepacked with Sephadex G-25 Fine resin for desalting and buffer exchange.
- Provides reliable and reproducible protein desalting and buffer exchanges of samples up to 15 mL.
- Two to four columns can be connected in series for sample sizes from 30 to 60 mL.
- Replaces dialysis for desalting protein, which saves time and reduces labor.
- HiPrep format compatible with peristaltic pumps or chromatography systems such as ÄKTA systems.
Column for desalting and buffer exchange of proteins
HiPrep 26/10 Desalting is a prepacked, ready-to-use column for group separation between high and low molecular weight substances. Applications include buffer exchange prior to or after different chromatographic steps, removal of low molecular weight contaminants, or removal of reagents to terminate a reaction.
| Parameter | HiPrep 26/10 Desalting |
|---|---|
| Bed dimensions | 26 × 100 mm |
| Bed height | 100 mm |
| Bed volume | 53 |
| Column i.d. | 26 mm |
| Flow rate1 | < 31 ml/min |
| Flow velocity | < 450 cm/h |
| Pressure max. (over the packed bed during operation) | 1.5 bar [0.15 MPa] (22 psi) |
| Sample volume | <= 15 ml |
| Storage | 4 to 30°C, 20% Ethanol |
| Pack size | 53 mL |
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文献和实验3 times with Buffer D (ATP saturation described further in Ref 3 ). 4. Acquire a Polytron or other homogenizer. 5. Have DNaseI-Sepharose column equilibrated in Buffer G and hooked in tandem with a G-25 Sephadex column also equilibrated in Buffer G
，G25，G50等去除小分子，效率高，处理量可达床体积30%。只需在进样后收集首1/3-1/2柱体积的洗脱液，就可以去除该填料分离范围上限以下的小分子，简单直接。由于只是去除小分子，柱高10cm以上即可。整个过程一般可于数分钟至半小时完成。Sephadex G25系列介质专为蛋白质脱盐而设计，预装柱HiTrap Desalting（5ml）可用针筒操作。HiPrep Desalting(26ml)可在数分钟为多至10ml样品脱盐。 16.疫苗纯化 使用凝胶过滤介质Sepharose 4FF
上疏水层析。若作离子交换，需先用Sephadex G-25脱盐。疏水层析是较新技术，随着介质种类不断增多，渐被融入各生产工艺中。利用Hitrap HIC Test Kit 和RESOURCE HIC Test Kit可在八种疏水介质中选择最适合介质及最佳的纯化条件。低盐洗脱的样品可稍加稀释或直接上其它吸附性层析。5.纯水-----糖类分子固化外源凝订素如刀豆球蛋白、花生、大麦等凝集素，可结合碳水化合物的糖类残基，很适合用作分离糖化细胞膜组份、细胞、甚至亚细胞细胞器，纯化糖蛋白等。两种附上外源凝集
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