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- 2025年11月19日
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Pkg of 100 capsules, each enough for 2.5 L enrichment broth for 250 g sample
Use RAPID'Salmonella Capsules for sample enrichment prior to the detection and enumeration of all Salmonellaspp. in products intended for human or animal consumption, or in environmental samples. After enrichment, identification using RAPID'Salmonella Medium is based on the chromogenic detection of C8-esterase activity, while simultaneous detection of β-glucosidase activity permits the differentiation of Salmonella from other Enterobacteriaceae.
Features and Benefits
- Capsules enable highly effective Salmonella growth, even when stressed, while limiting the growth of interfering flora
- Salmonella spp. form magenta colonies on RAPID'Salmonella Agar, while non-Salmonella spp. form blue or uncolored colonies
- Detect motile and non-motile Salmonella, lactose-positive Salmonella, and Salmonella Typhi/Paratyphi
- Results in <42 hr
Options for confirmation of Salmonella and serotype identification include the latex agglutination test (Salmonellagroups B to E and G), iQ-Check® Salmonella II Real-Time PCR Test, evaluation of cytochrome c oxidases, ONPG Test (β-galactosidase), and Bio-Rad’s extensive range of antisera to flagellar, somatic, and capsular antigens.
Validations
- Certified NF VALIDATION according to the ISO 16140 standard and validated by the AOAC
- RAPID'Salmonella Medium can be used as a second medium in the ISO 6579 standard
Packaging Options
RAPID'Salmonella Capsules, unconcentrated, are also available (3564710)
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文献和实验Single Primer ("Semi-Random") P
Reamplification 40 µL H2O 10 µL 10x M-PCRB 10 µL 4 dNTPs @ 2 mM each 10 µL Pn primer @ 5 µM 10 µL Po primer @ 0.2 µM 10 µL DNA from part 1 10 µL "T/TS" @ 0.4 u Taq/µL --------- 100 µL Load glass capillaries and amplify: 30"x94°C 30
of H2O:54 g of Tris base27.5 g of boric acid20 ml of 0.5 M EDTA (pH 8.0)The 0.5x working solution is 45 mM Tris-borate/1 mM EDTA.TBE is usually made and stored as a 5x or 10x stock solution. The pH of the concentrated stock buffer should be approx
Single Primer (Semi-Random) PCR
In our lab, PCR is always done in an Air ThermoCycler (Idaho Technologies, PO Box 50819, Idaho Falls, ID 83402), which ramps quickly, allowing short dwell times during denaturation and annealing. This enables rapid and stringent polymerization
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