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- SignalChem
- 加拿大
- K01-09-05
- 2025年07月11日
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- 详细信息
- 文献和实验
- 技术资料
- 供应商：
安诺伦（北京）生物科技有限公司
- 规格：
5 ml
存储溶液：Frozen solution of buffer containing: 25 mM MOPS, pH7.2, 12.5mM B-glycerol-phosphate, 25mM MgCl2, 5mM EGTA, 2mM EDTA, 0.25mM DTT.
应用：Kinase Assay
官网链接：https://www.signalchem.com/product_details.php?id=370
商品关键词：Kinase Assay Buffer I,K01-09-05,SignalChem
简单描述：Stock solution of buffer used for assaying kinase protein.
Sterility: Buffer passed through a 0.2µ filter membrane and packaged aseptically.
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文献和实验is lost on tips). Rotate in cold room for 1 hr. Spin and wash beads twice with RIPA (discard supernatant), and then once with Histone Wash Buffer. Add 30 µL of Histone Assay solution. Incubate at 37°C for exactly 30 min. Mix
Immunoprecipitation and Immune Complex kinase assay
9) Transfer to a new eppi tube and wash IP 3x with 500 microliters lysis buffer and then 1x with TN by spinning and resuspending. 10) If doing kinase assay, do additional wash in TN and then proceed with kinase assay (below). 11) Pellets can be stored dry
Direct Kinase Assay Screening for Inhibitors of MAP Kinase
-myristate 13-acetate) (8 ), antigen binding to T-cell receptors, and infection of cells by Salmonella sp. MAP kinase is also involved in cell-cycle control and becomes activated during M-phase of the cell cycle (9 ). As such, MAP kinase is of great
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