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- SignalChem
- 加拿大
- K314-911-05
- 2025年07月13日
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- 详细信息
- 文献和实验
- 技术资料
- 供应商：
安诺伦（北京）生物科技有限公司
- 规格：
5 nmol
存储温度：The lyophilized powder is stable for at least 4 weeks at room temperature. It is recommended that the lyophilized and resuspended siRNAs are stored at or below -20oC. After resuspension, siRNA stock solutions ≥2 uM can undergo up to 50 freeze-thaw cycles without significant degradation. For long-term storage, it is recommended that the siRNA is stored at -70oC. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
存储溶液：The siRNAs are supplied as a lyophilized powder and shipped at room temperature.
参考文献：1. Kamine, J. et.al: Identification of a cellular protein that specifically interacts with the essential cysteine region of the HIV-1 Tat transcriber. Virology 216: 357-366, 1996. 2. Gorrini, C. et.al: Tip60 is a haplo-insufficient tumour suppressor required for an oncogene-induced DNA damage response. Nature 448: 1063-1067, 2007.
官网链接：https://www.signalchem.com/product_details.php?id=7636
商品关键词：KAT5 (TIP60) siRNA Set I,,K314-911-05,SignalChem
简单描述：KAT5 (TIP60) is a pool of three individual synthetic siRNA duplexes designed to knock-down human KAT5 (TIP60) mRNA expression. Each siRNA is 19-25 bases in length.
Description: KAT5 (TIP60) is a pool of three individual synthetic siRNA duplexes designed to knock-down human KAT5 (TIP60) mRNA expression. Each siRNA is 19-25 bases in length.
Reconstitution Protocol: Briefly centrifuge the tubes (maximum RCF 4,000g) to collect lyophilized siRNA at the bottom of the tube. Resuspend the siRNA in 50 ul of DEPC-treated water (supplied by researcher), which results in a 1x stock solution (10 uM). Gently pipet the solution 3-5 times to mix and avoid the introduction of bubbles. Optional: aliquot 1x stock solutions for storage.
Format: Lyophilized powder
Gene Aliases: cPLA2; ESA1; HTATIP; HTATIP1; PLIP; TIP; TIP60
官网链接: www.annoron.com
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文献和实验Our current strategy with siRNA is to synthesis relatively small amounts enzymatically and use these to test for efficiency by western blotting. This allows us to test several candidate siRNAs quite cheaply. The yields are good enough for reasonable
starter kits that include a set of controls for the siRNA transfection and Western blot analysis. Transfection of siRNA duplexes We perform a single transfection of siRNA duplex using OLIGOFECTAMINE Reagent (product number: 12252011
and now provide validated sets of siRNA reagents together with the corresponding target-specific antibody. They also provide siRNA starter kits that include a set of controls for the siRNA transfection and Western blot analysis. Transfection of siRNA duplexes
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