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- 详细信息
- 技术资料
- 供应商:
上海联迈生物工程有限公司
- 库存:
大量
- 目录编号:
LM-8662R
- 克隆性:
多克隆
- 抗原来源:
Rabbit
- 保质期:
1年
- 抗体英文名:
BASP1
- 抗体名:
脑富含膜附着信号蛋白1抗体
- 宿主:
Rabbit
- 适应物种:
Human, Mouse, Rat, Chicken, Dog, Pig, Cow, Horse, Rabbit,
- 免疫原:
KLH conjugated synthetic peptide derived from human BASP1/Nap22:3-100/227
- 亚型:
IgG
- 形态:
Lyophilized or Liquid
- 应用范围:
WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 ICC=1:100-500 IF=1:100-500 (石蜡切片需做抗原修复)
- 浓度:
1mg/ml
- 保存条件:
Store at -20 °C
- 规格:
100ul 200ul
BASP1脑富含膜附着信号蛋白1抗体
| 英文名称 | BASP1 |
| 中文名称 | 脑富含膜附着信号蛋白1抗体 |
| 别 名 | BASP 1; BASP-1; BASP1 protein; Brain abundant membrane attached signal protein 1; Brain Abundant Signal Protein Membrane Attached 1; Brain Acid Soluble Protein 1; CAP 23; CAP23; MGC8555; NAP 22; NAP-22; NAP22; Neuronal axonal membrane protein NAP 22; Neuronal tissue enriched acidic protein; 22 kDa neuronal tissue enriched acidic protein; BASP1_HUMAN. |
| 规格价格 | 100ul/1380元 购买 200ul/2200元 购买 大包装/询价 |
| 说 明 书 | 100ul 200ul |
| 研究领域 | 细胞生物 神经生物学 表观遗传学 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Human, Mouse, Rat, Chicken, Dog, Pig, Cow, Horse, Rabbit, |
| 产品应用 | WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 ICC=1:100-500 IF=1:100-500 (石蜡切片需做抗原修复) not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 分 子 量 | 25kDa |
| 细胞定位 | 细胞膜 |
| 性 状 | Lyophilized or Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human BASP1/Nap22:3-100/227 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 储 存 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存条件 | Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. |
| PubMed | PubMed |
| 产品介绍 | background: Neuronal axonal membrane protein Nap-22, also designated neuronal tissue-enriched acidic protein or brain acid soluble protein (BASP1), is a Ca2+-dependent calmodulin-binding protein that is important for neuronal sprouting and plasticity. Nap-22 is abundant in brain nerve terminals and is also present in significant amounts in kidney, testis and lymphoid tissue. Nap-22 undergoes N-terminal myristoylation for membrane localization. It has been characterized as a major protein of neuronal rafts, which are known to preferentially bind membranes containing cholesterol. Nap-22 is a crucial protein active in neurite outgrowth and synaptic plasticity. Function: BASP1 is a brain abundant membrane bound protein with several transient phosphorylation sites and PEST motifs. BASP1 binds calmodulin, is phosphorylated by protein kinase C and undergoes N-terminal myristoylation. BASP1 is expressed specifically in nervous tissue. Intercellular signaling and nerve outgrowth are influenced by a number of proteins expressed at nerve endings and synapses of which BASP1 is one. Subcellular Location: Cell membrane; Lipid-anchor. Cell projection, growth cone. Note=Associated with the membranes of growth cones that form the tips of elongating axons. Tissue Specificity: Brain Similarity: Belongs to the BASP1 family. SWISS: P80723 Gene ID: 10409 Database links: Entrez Gene: 10409 Human Entrez Gene: 70350 Mouse Omim: 605940 Human SwissProt: P80723 Human SwissProt: Q91XV3 Mouse Unigene: 201641 Human Unigene: 29586 Mouse Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 产品图片 | ![]() Sample:Hela (Human)Cell Lysate at 40 ug Primary: Anti-BASP1(bs-8662R)at 1/300 dilution Secondary: IRDye800CW Goat Anti-RabbitIgG at 1/20000 dilution Predicted band size: 25kD Observed band size: 26kD ![]() Paraformaldehyde-fixed, paraffin embedded (mouse brain tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (BASP1) Polyclonal Antibody, Unconjugated (bs-8662R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining. ![]() Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-BASP1 Polyclonal Antibody, Unconjugated(bs-8662R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining |
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BASP1脑富含膜附着信号蛋白1抗体
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