- ￥3988
- Biogems
- 500-P33,50μg
- 2025年07月09日
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- 详细信息
- 文献和实验
- 技术资料
- 克隆性：
多克隆
- 供应商：
安诺伦（北京）生物科技有限公司
- 靶点：
Recombinant Human GM-CSF (BioGems Catalog #300-03)
- 规格：
50μg
存储溶液：purified
官网链接：http://www.bio-gems.com/recombinant-proteins/anti-cytokine-chemokine-antibodies/anti-human-gm-csf.html
商品关键词：Anti-Human GM-CSF,500-P33,50μg,
简单描述：Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hGM-CSF. Anti-Human GM-CSF specific antibody was purified by affinity chromatography employing immobilized hGM-CSF matrix.
Immunohistochemistery: This antibody stained formalin-fixed, paraffin-embedded sections of human cervical squamous cell carcinoma. The recommended concentration is 0.25 µg/mL with an overnight incubation at 4°C. An HRP-labeled polymer detection system was used with a DAB chromogen. Heat induced antigen retrieval with a pH 6.0 sodium citrate buffer is recommended. Optimal concentrations and conditions may vary.
Sandwich ELISA: To detect hGM-CSF by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.5 - 2.0 μg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with BioGems' Biotinylated Anti-Human GM-CSF (60-167BT) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hGM-CSF.
Western Blot: To detect hGM-CSF by Western Blot analysis this antibody can be used at a concentration of 0.1- 0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hGM-CSF is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity of hGM-CSF (1.0 ng/ml), a concentration of 0.13-0.20 µg/ml of this antibody is required.
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文献和实验a simple ELISA method to detect human anti-antibody response.
Immunohistochemistry: Mouse Anti-Human MCP-1
. 4. Incubate the tissue section overnight at 4?C with Mouse Anti-Human MCP-1 at 10.0 μg/mL. Wash the slide twice for three minutes each. 5. Incubate the tissue section with the Envision HRP polymer kit (Dako) according to the manufacturer’s
Synthesis of Recombinant Human Cytokine GM-CSF in the Seeds of Transgenic Tobacco Plants
, the probability of health risks because of contamination with potential human pathogens and toxins in recombinant products derived from plants is minimized. Furthermore, plants have glycosylation machinery like other eukaryotic systems (1 ).
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