- ￥3860
- Biogems
- 500-P246,100μg
- 2025年07月12日
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- 详细信息
- 文献和实验
- 技术资料
- 克隆性：
多克隆
- 供应商：
安诺伦（北京）生物科技有限公司
- 靶点：
Recombinant Human Galectin-3 (BioGems Catalog #450-38)
- 规格：
100μg
存储溶液：purified
官网链接：http://www.bio-gems.com/recombinant-proteins/anti-cytokine-chemokine-antibodies/anti-human-galectin-3.html
商品关键词：Anti-Human Galectin-3,500-P246,100μg ,Biogems
简单描述：Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hGalectin-3. Anti-Human Galectin-3 specific antibody was purified by affinity chromatography employing immobilized hGalectin-3 matrix.
Immunohistochemistery: This antibody stained formalin-fixed paraffin-embedded sections of human pancreas infiltrating ductal adenocarcinoma tissue. The recommended concentration is 31.25 ng/mL with an overnight incubation at 4°C. An HRP-labeled polymer detection system was used with a DAB chromogen. Optimal results for these conditions were achieved with heat induced antigen retrieval with a pH 6.0 sodium citrate buffer. Optimal concentrations and conditions may vary.
Sandwich ELISA: To detect hGalectin-3 by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.5 - 2.0 μg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with BioGems' Biotinylated Anti-Human Galectin-3 (60-155BT) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hGalectin-3.
Western Blot: To detect hGalectin-3 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hGalectin-3 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
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文献和实验a simple ELISA method to detect human anti-antibody response.
Immunohistochemistry: Mouse Anti-Human MCP-1
. 4. Incubate the tissue section overnight at 4?C with Mouse Anti-Human MCP-1 at 10.0 μg/mL. Wash the slide twice for three minutes each. 5. Incubate the tissue section with the Envision HRP polymer kit (Dako) according to the manufacturer’s
Galectin-3 Binding and Metastasis
in transformed and metastatic cell lines (Raz and Lotan Cancer Metastasis Rev 6: 433–452, 1987; Raz et al. Int J Cancer 46: 871–877, 1990). Moreover, in many human carcinomas, an increased expression of galectin-3 correlates with progressive tumor stages (Lotan
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