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- 详细信息
- 文献和实验
- 技术资料
- 供应商:
上海联迈生物工程有限公司
- 库存:
大量
- 目录编号:
LM-7696R
- 克隆性:
多克隆
- 抗原来源:
Rabbit
- 保质期:
1年
- 抗体英文名:
AXUD1
- 抗体名:
转化生长因子β诱导蛋白3抗体(半胱氨酸和丝氨酸富含核蛋白1)
- 宿主:
Rabbit
- 适应物种:
Human, Mouse, Rat, Pig, Cow, Rabbit, Sheep,
- 免疫原:
KLH conjugated synthetic peptide derived from human AXUD1:201-300/589
- 亚型:
IgG
- 形态:
Lyophilized or Liquid
- 应用范围:
ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 Flow-Cyt=1ug/Test IF=1:100-500 (石蜡切片需做抗原修复)
- 浓度:
1mg/ml
- 保存条件:
Store at -20 °C
- 规格:
100ul 200ul
| 英文名称 | AXUD1 |
| 中文名称 | 转化生长因子β诱导蛋白3抗体(半胱氨酸和丝氨酸富含核蛋白1) |
| 别 名 | Axin 1 Up-regulated; Axin-1 up-regulated gene 1 protein; CSRN1_HUMAN; CSRNP-1; CSRNP1; Cysteine/serine-rich nuclear protein 1; DKFZp566F164; FAM130B; Protein URAX1; TAIP 3; TAIP-3; TGF beta induced apoptosis protein 3; TGF-beta-induced apoptosis protein 3; URAX1. |
| 规格价格 | 100ul/1380元 购买 200ul/2200元 购买 大包装/询价 |
| 说 明 书 | 100ul 200ul |
| 研究领域 | 细胞生物 信号转导 细胞周期蛋白 转录调节因子 表观遗传学 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Human, Mouse, Rat, Pig, Cow, Rabbit, Sheep, |
| 产品应用 | ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 Flow-Cyt=1ug/Test IF=1:100-500 (石蜡切片需做抗原修复) not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 分 子 量 | 64kDa |
| 细胞定位 | 细胞核 |
| 性 状 | Lyophilized or Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human AXUD1:201-300/589 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 储 存 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存条件 | Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. |
| PubMed | PubMed |
| 产品介绍 | background: This gene encodes a protein that localizes to the nucleus and expression of this gene is induced in response to elevated levels of axin. The Wnt signalling pathway, which is negatively regulated by axin, is important in axis formation in early development and impaired regulation of this signalling pathway is often involved in tumors. A decreased level of expression of this gene in tumors compared to the level of expression in their corresponding normal tissues suggests that this gene product has a tumor suppressor function. [provided by RefSeq, Jul 2008]. Function: Binds to the consensus sequence 5'-AGAGTG-3' and has transcriptional activator activity. May have a tumor-suppressor function. May play a role in apoptosis. Subcellular Location: Nucleus Tissue Specificity: Ubiquitous. Most abundantly expressed in lung, placenta, skeletal muscle, pancreas and leukocyte. Frequently down-regulated in lung, kidney, liver and colon cancers compared with their corresponding normal tissues. Similarity: Belongs to the AXUD1 family. SWISS: Q96S65 Gene ID: 64651 Database links: UniProtKB/Swiss-Prot: Q96S65.2 Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 产品图片 | ![]() Blank control (blue line): Mouse thymus cells(Black). Primary Antibody (green line): Rabbit Anti-AXUD1 antibody (bs-7696R) Dilution: 1μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody (white blue line): Goat anti-rabbit IgG-FITC Dilution: 1μg /test. Protocol: The Cells fixed with 4% paraformaldehyde,permeabilized with0.1% PBST.Then stained with Primary Antibody for 30 min at room temperature.The cells were then incubated in 1 X PBS/2%BSA/10% goat serum to block non-specific protein-protein interactions followed by the antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed. |
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文献和实验进行全面审查。因此,本概述仅涉及当今蛋白质研究中研究的少数最常见类型的 PTM。此外,更多的重点放在磷酸化、糖基化和泛素化,因此这些 PTM 在各自的 PTM 专页中进行了更详细的描述。1、Phosphorylation:可逆的蛋白质磷酸化,主要发生在丝氨酸、苏氨酸或酪氨酸残基上,是目前研究得最深入的翻译后修饰之一。磷酸化在细胞周期、生长、凋亡和信号转导等过程中起着重要的调控作用。在以下示例中,使用蛋白免疫印迹分析评价分别用表皮生长因子 (EGF) 和血小板衍生生长因子 (PDGF) 刺激血清
基酸彼此同源。均含有2个拷贝的SCR和1个拷贝的EGF前体结构功能域(图5-18)。 图5-18 4种丝氨酸蛋白酶补体蛋白的结构功能域 C2和Bf均为单肽链糖蛋白,它们除在形成两条补体激活途径中和C3转化酶方面十分相似外,在合成部位、合成途径、分子大小、亚单位结构、半胱氨酸位置及数目,以及保守残基替代及活性部位等方面也有很大的相似性(表5-4)。C2和Bf分子中相同的结构功能域是均有3个CSR、1个与von Willebrand因子(vWF)共同
中科院高福院士课题组 2020 年发表了哪些重要的研究成果?
。相反,PD-1 或 PD-L1 的过表达抑制肿瘤细胞增殖和集落形成。此外,靶向 PD-1 或 PD-L1 的封闭抗体会促进细胞培养和异种移植物中肿瘤的生长。从机制上讲,PD-1 和 PD-L1 的协调激活了其主要的下游信号传导途径,包括 AKT 和 ERK1 / 2 途径,从而增强了肿瘤细胞的生长。这项研究表明,PD-1 / PD-L1 是潜在的肿瘤抑制因子,并且潜在地调节了对抗 PD-1 / PD-L1 治疗的反应,因此代表了最佳癌症免疫治疗方法的潜在生物标志物。 图源:PNAS 4、揭示沙粒
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