手机验证
上海联迈生物工程有限公司
大量
LM-33296M
多克隆
Rabbit
1年
Ubiquitin
泛素蛋白单克隆抗体
Rabbit
Human, Mouse, Rat,
KLH conjugated synthetic peptide derived from human Ubiquitin:
IgG
Lyophilized or Liquid
IHC-P=1:400-800 IHC-F=1:400-800 ICC=1:100-500 IF=1:100-500 (石蜡切片需做抗原修复)
1mg/ml
Store at -20 °C
50ul 100ul
英文名称 | Ubiquitin |
中文名称 | 泛素蛋白单克隆抗体 |
别 名 | FLJ25987; MGC8385; RPS27A; RPS 27A; UBA52; UBA 52; UBA80; UBA 80; UBB; UBC; UB; UBCEP1; UBCEP2; UBCEP 1; UBCEP 2; UBCEP-1; UBCEP-2; Ubiquitin; ubiquitin B; UBC_HUMAN; UBB_HUMAN; RS27A_HUMAN; HMG20; Ubiquitin carboxyl extension protein 80; Ubiquitin; Polyubiquitin-C; Polyubiquitin-B. |
规格价格 | 50ul/860元 购买 100ul/1580元 购买 大包装/询价 |
说 明 书 | 50ul 100ul |
研究领域 | 肿瘤 细胞生物 神经生物学 细胞凋亡 泛素 |
抗体来源 | Mouse |
克隆类型 | Monoclonal |
克 隆 号 | 7H3 |
交叉反应 | Human, Mouse, Rat, |
产品应用 | IHC-P=1:400-800 IHC-F=1:400-800 ICC=1:100-500 IF=1:100-500 (石蜡切片需做抗原修复) not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
分 子 量 | 8.5kDa |
细胞定位 | 细胞核 细胞浆 |
性 状 | Lyophilized or Liquid |
浓 度 | 1mg/ml |
免 疫 原 | KLH conjugated synthetic peptide derived from human Ubiquitin: |
亚 型 | IgG |
纯化方法 | affinity purified by Protein G |
储 存 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
保存条件 | Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. |
PubMed | PubMed |
产品介绍 | background: This gene encodes ubiquitin, one of the most conserved proteins known. Ubiquitin has a major role in targeting cellular proteins for degradation by the 26S proteosome. It is also involved in the maintenance of chromatin structure, the regulation of gene expression, and the stress response. Ubiquitin is synthesized as a precursor protein consisting of either polyubiquitin chains or a single ubiquitin moiety fused to an unrelated protein. This gene consists of three direct repeats of the ubiquitin coding sequence with no spacer sequence. Consequently, the protein is expressed as a polyubiquitin precursor with a final amino acid after the last repeat. An aberrant form of this protein has been detected in patients with Alzheimer's disease and Down syndrome. Pseudogenes of this gene are located on chromosomes 1, 2, 13, and 17. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2013] Function: Ubiquitin exists either covalently attached to another protein, or free (unanchored). When covalently bound, it is conjugated to target proteins via an isopeptide bond either as a monomer (monoubiquitin), a polymer linked via different Lys residues of the ubiquitin (polyubiquitin chains) or a linear polymer linked via the initiator Met of the ubiquitin (linear polyubiquitin chains). Polyubiquitin chains, when attached to a target protein, have different functions depending on the Lys residue of the ubiquitin that is linked: Lys-6-linked may be involved in DNA repair; Lys-11-linked is involved in ERAD (endoplasmic reticulum-associated degradation) and in cell-cycle regulation; Lys-29-linked is involved in lysosomal degradation; Lys-33-linked is involved in kinase modification; Lys-48-linked is involved in protein degradation via the proteasome; Lys-63-linked is involved in endocytosis, DNA-damage responses as well as in signaling processes leading to activation of the transcription factor NF-kappa-B. Linear polymer chains formed via attachment by the initiator Met lead to cell signaling. Ubiquitin is usually conjugated to Lys residues of target proteins, however, in rare cases, conjugation to Cys or Ser residues has been observed. When polyubiquitin is free (unanchored-polyubiquitin), it also has distinct roles, such as in activation of protein kinases, and in signaling. Subcellular Location: Cytoplasm. Nucleus. Similarity: Belongs to the ubiquitin family. Contains 9 ubiquitin-like domains. SWISS: P0CG48 Gene ID: 7314 Database links: Entrez Gene: 281370 Cow Entrez Gene: 100049794 Horse Entrez Gene: 7314 Human Entrez Gene: 22187 Mouse Entrez Gene: 192255 Rat Omim: 191339 Human SwissProt: P0CG53 Cow SwissProt: Q8MKD1 Horse SwissProt: P0CG47 Human SwissProt: P0CG48 Human SwissProt: P62988 Human SwissProt: P0CG49 Mouse SwissProt: P62991 Mouse SwissProt: P0CG51 Rat SwissProt: P62989 Rat Unigene: 356190 Human Unigene: 520348 Human Unigene: 282093 Mouse Unigene: 371592 Mouse Unigene: 110618 Rat Unigene: 1253 Rat Unigene: 3761 Rat Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
产品图片 | Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Ubiquitin) Monoclonal Antibody, Unconjugated (ascites of bsm-33296M 7H3) at 1:2000 overnight at 4°C, followed by a conjugated secondary (sp-0024) for 20 minutes and DAB staining. Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Ubiquitin) Monoclonal Antibody, Unconjugated (bsm-33296M-7H3) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Mouse) (sp-0023) instructionsand DAB staining. |
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