- ¥1580
- LMAI Bio
- LM-7523R
- 进口/国产
- 2025年12月30日
- WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 IF=1:100-500 （石蜡切片需做抗原修复）
- Rabbit
- Mouse, Rat,
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- 详细信息
- 文献和实验
- 技术资料
- 供应商：
上海联迈生物工程有限公司
- 库存：
大量
- 目录编号：
LM-7523R
- 克隆性：
多克隆
- 抗原来源：
Rabbit
- 保质期：
1年
- 抗体英文名：
phospho-Caveolin-2 (Tyr19)
- 抗体名：
磷酸化细胞质膜微囊蛋白-2抗体
- 宿主：
Rabbit
- 适应物种：
Mouse, Rat,
- 免疫原：
KLH conjugated Synthesised phosphopeptide derived from mouse Caveolin2 around the phosphorylation site of Tyr19.:DA(p-Y)SH
- 亚型：
IgG
- 形态：
Lyophilized or Liquid
- 应用范围：
WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 IF=1:100-500 （石蜡切片需做抗原修复）
- 浓度：
1mg/ml
- 保存条件：
Store at -20 °C
- 规格：
100ul
| 英文名称 | phospho-Caveolin-2 (Tyr19) |
| 中文名称 | 磷酸化细胞质膜微囊蛋白-2抗体 |
| 别 名 | Caveolin 2 (phospho Y19); p-Caveolin 2(phospho Y19); CAV; CAV2; CAV2_HUMAN; Caveolae protein 20 kD; Caveolin 2; Caveolin 2 isoform a and b; Caveolin 2 isoform c; Caveolin-2; MGC12294; CAV2_HUMAN. |
| 规格价格 | 100ul/1580元 购买 大包装/询价 |
| 说 明 书 | 100ul |
| 产品类型 | 磷酸化抗体 |
| 研究领域 | 心血管 细胞生物 信号转导 细胞凋亡 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Mouse, Rat, |
| 产品应用 | WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 IF=1:100-500 （石蜡切片需做抗原修复） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 分 子 量 | 18kDa |
| 细胞定位 | 细胞核 细胞浆 细胞膜 |
| 性 状 | Lyophilized or Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated Synthesised phosphopeptide derived from mouse Caveolin2 around the phosphorylation site of Tyr19.:DA(p-Y)SH |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 储 存 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存条件 | Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. |
| PubMed | PubMed |
| 产品介绍 | background: Cellular localization: Nucleus. Cytoplasm. Golgi apparatus membrane. Cell membrane. Membrane > caveola. Potential hairpin-like structure in the membrane. Membrane protein of caveolae. Tyr-19-phosphorylated form is enriched at sites of cell-cell contact and is translocated to the nucleus in complex with MAPK1 in response to insulin (By similarity). Tyr-27-phosphorylated form is located both in the cytoplasm and plasma membrane. CAV1-mediated Ser-23-phosphorylated form locates to the plasma membrane. Ser-36-phosphorylated form resides in intracellular compartments. Function: May act as a scaffolding protein within caveolarmembranes. Interacts directly with G-protein alpha subunits and canfunctionally regulate their activity. Acts as an accessory proteinin conjunction with CAV1 in targeting to lipid rafts and drivingcaveolae formation. The Ser-36 phosphorylated form has a role inmodulating mitosis in endothelial cells. Positive regulator ofcellular mitogenesis of the MAPK signaling pathway. Required forthe insulin-stimulated nuclear translocation and activation ofMAPK1 and STAT3, and the subsequent regulation of cell cycleprogression (By similarity). Subunit: Monomer or homodimer. Interacts with CAV1; theinteraction forms a stable heterooligomeric complex that isrequired for targeting to lipid rafts and for caveolae formation.Tyrosine phosphorylated forms do not form heterooligomers with theTyr-19-phosphorylated form existing as a monomer or dimer, and theTyr-27-form as a monomer only. Interacts (tyrosine phosphorylatedform) with the SH2 domain-containing proteins, RASA1, NCK1 and SRC.Interacts (tyrosine phosphorylated form) with INSR, the interaction(Tyr-27-phosphorylated form) is increased on insulin stimulation.Interacts (Tyr-19 phosphorylated form) with MAPK1 (phosphorylatedform); the interaction, promoted by insulin, leads to nuclearlocation and MAPK1 activation. Interacts with STAT3; theinteraction is increased on insulin-induced tyrosinephosphorylation leading to STAT activation (By similarity). Subcellular Location: Nucleus. Cytoplasm. Golgi apparatusmembrane; Peripheral membrane protein. Cell membrane; Peripheralmembrane protein. Membrane, caveola; Peripheral membrane protein.Note=Potential hairpin-like structure in the membrane. Membraneprotein of caveolae. Tyr-19-phosphorylated form is enriched atsites of cell-cell contact and is translocated to the nucleus incomplex with MAPK1 in response to insulin (By similarity).Tyr-27-phosphorylated form is located both in the cytoplasm andplasma membrane. CAV1-mediated Ser-23-phosphorylated form locatesto the plasma membrane. Ser-36-phosphorylated form resides inintracellular compartments. Tissue Specificity: Expressed in endothelial cells, smooth musclecells, skeletal myoblasts and fibroblasts. Post-translational modifications: Phosphorylated on serine and tyrosine residues. CAV1 promotesphosphorylation on Ser-23 which then targets the complex to theplasma membrane, lipid rafts and caveolae. Phosphorylation onSer-36 appears to modulate mitosis in endothelial cells (Bysimilarity). Phosphorylation on both Tyr-19 and Tyr-27 is requiredfor insulin-induced 'Ser-727' phosphorylation of STAT3 and itsactivation. Phosphorylation on Tyr-19 is required forinsulin-induced phosphorylation of MAPK1 and DNA binding of STAT3.Tyrosine phosphorylation is induced by both EGF and insulin (Bysimilarity). Similarity: Belongs to the caveolin family. SWISS: Q9WVC3 Gene ID: 12390 Database links: Entrez Gene: 858 Human Entrez Gene: 12390 Mouse Entrez Gene: 100362824 Rat Entrez Gene: 363425 Rat Omim: 601048 Human SwissProt: P51636 Human SwissProt: Q9WVC3 Mouse SwissProt: Q2IBC5 Rat Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. Caveolin是细胞生长相关信号途径及肿瘤发生发展过程中重要的抑制因子，Caveolae是细胞膜内的特殊膜结构，参与包括细胞的分子运输、细胞粘附和信号转导在内的多种细胞活动。Caveolin-1是Caveolae中重要的结构蛋白，抑制细胞生长，与多种人类肿瘤发生发展相关的信号分子相互作用。Caveolin在信号转导的整合中起支架蛋白的作用。Caveolin构成了一个蛋白家族，他们是细胞质膜中发夹样结构域的主要结构成分。Caveolin在信号转导的整合中起支架蛋白的作用。至今已经鉴定了3种Caveolin （Caveolin-1、2 和3），它们具有不同的组织分布。 |
| 产品图片 |  Sample: Heart (Rat) Lysate at 40 ug Primary: Anti-phospho-Caveolin-2 (Tyr19) (bs-7523R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 18 kD Observed band size: 18 kD |
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文献和实验Using Phospho‐Motif Antibodies to Determine Kinase Substrates
the phosphorylation site; therefore, substrate?directed, phosphorylation?state?sensitive, motif?specific (?phospho?motif?) antibodies represent powerful tools to identify novel kinase substrates and to investigate mechanisms of substrate phosphorylation
（3）同上。两个磷酸化位点都要做，但是可能在不同的病理条件下，侧重点有所不同，这主要体现在你的论文的讨论中。因此建议实验前多做论文研究，查阅一下别人的发表文章，看看与你的病理模型更相关的是哪个位点。 真爱满行囊 非常感谢你 的回复，现在我遇到的问题是我做出来蛋白总量还有216位点是有变化的，9位因为买的是santa curz的抗体，现在死活做不出来，很头痛，我要说明的问题是他的活性降低，我也看到216位点磷酸化水平降低了，可是现在没有第九位的结果（就是没有办法得到第九
米宝 这俩天做实验，极其郁闷。我做的总AKt和pAkt,大鼠脑组织，第一次跑总AKT的时候，我觉得可能是因为蛋白是提取的浓度比较好吧，出来了一条浓浓的条带，三组总AKt都出了，接下来做pAkt,就出了一个带，效果挺明显的。后来再做pAkt的时候，跑了4张膜，显影的时候，太干净了，上面什么都没有，甚至连个杂带，斑点 都没！实验是在实验员的领导下完成的，技术上基本没什么纰漏，查阅本版面有关这问题方面的介绍，有的解释说pakt降解了！一抗是用的Phospho-Akt
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