- ¥1380 - 2200
- LMAI Bio
- LM-7004R
- 进口/国产
- 2025年11月27日
- ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 Flow-Cyt=0.2ug/Test ICC=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复）
- Rabbit
- Human, Mouse,
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- 详细信息
- 文献和实验
- 技术资料
- 供应商：
上海联迈生物工程有限公司
- 库存：
大量
- 目录编号：
LM-7004R
- 克隆性：
多克隆
- 抗原来源：
Rabbit
- 保质期：
1年
- 抗体英文名：
PRAME
- 抗体名：
黑色素瘤优先表达抗原抗体
- 宿主：
Rabbit
- 适应物种：
Human, Mouse,
- 免疫原：
KLH conjugated synthetic peptide derived from human PRAME (174-210aa):151-250/509
- 亚型：
IgG
- 形态：
Lyophilized or Liquid
- 应用范围：
ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 Flow-Cyt=0.2ug/Test ICC=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复）
- 浓度：
1mg/ml
- 保存条件：
Store at -20 °C
- 规格：
100ul 200ul
| 英文名称 | PRAME |
| 中文名称 | 黑色素瘤优先表达抗原抗体 |
| 别 名 | MAPE; Melanoma antigen preferentially expressed in tumors; OIP 4; OIP-4; OIP4; OPA interacting protein 4; Opa interacting protein OIP4; OPA-interacting protein 4; PRAME; PRAME_HUMAN; Preferentially expressed antigen in melanoma; Preferentially expressed antigen of melanoma; RP23-250F8.3. |
| 规格价格 | 100ul/1380元 购买 200ul/2200元 购买 大包装/询价 |
| 说 明 书 | 100ul 200ul |
| 研究领域 | 肿瘤 细胞生物 信号转导 肿瘤细胞生物标志物 表观遗传学 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Human, Mouse, |
| 产品应用 | ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 Flow-Cyt=0.2ug/Test ICC=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 分 子 量 | 56kDa |
| 细胞定位 | 细胞核 细胞膜 |
| 性 状 | Lyophilized or Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human PRAME (174-210aa):151-250/509 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 储 存 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存条件 | Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. |
| PubMed | PubMed |
| 产品介绍 | background: This gene encodes an antigen that is predominantly expressed in human melanomas and that is recognized by cytolytic T lymphocytes. It is not expressed in normal tissues, except testis. This expression pattern is similar to that of other CT antigens, such as MAGE, BAGE and GAGE. However, unlike these other CT antigens, this gene is also expressed in acute leukemias. Five alternatively spliced transcript variants encoding the same protein have been observed for this gene. [provided by RefSeq, Jul 2008]. Function: Functions as a transcriptional repressor, inhibiting the signaling of retinoic acid through the retinoic acid receptors RARA, RARB and RARG. Prevents retinoic acid-induced cell proliferation arrest, differentiation and apoptosis. Subunit: Interacts with RARA (via the ligand-binding domain); the interaction is direct and ligand (retinoic acid)-dependent. Interacts with EZH2; required to repress RAR signaling. Subcellular Location: Nucleus (Probable). Cell membrane. Tissue Specificity: Expressed in testis. Detected in samples of kidney, brain and skin. Similarity: Belongs to the PRAME family. Contains 4 LRR (leucine-rich) repeats. SWISS: P78395 Gene ID: 23532 Database links: Entrez Gene: 23532 Human Omim: 606021 Human SwissProt: P78395 Human Unigene: 30743 Human Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 产品图片 |  Tissue/cell: mouse lung tissue(Melanoma); 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-PRAME Polyclonal Antibody, Unconjugated(bs-7004R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining  Blank control(blue): Hela Cells(fixed with 2% paraformaldehyde (10 min) and then permeabilized with ice-cold 90% methanol for 30 min on ice). Primary Antibody: Rabbit Anti-PRAME/AF647 Conjugated antibody (bs-7004R-AF647), Dilution: 0.2μg in 100 μL 1X PBS containing 0.5% BSA; Isotype Control Antibody: Rabbit IgG/AF647(orange) ,used under the same conditions.  Blank control(blue): Hela Cells(fixed with 2% paraformaldehyde (10 min) and then permeabilized with ice-cold 90% methanol for 30 min on ice). Primary Antibody: Rabbit Anti-PRAME/AF647 Conjugated antibody (bs-7004R-AF647), Dilution: 0.2μg in 100 μL 1X PBS containing 0.5% BSA; Isotype Control Antibody: Rabbit IgG/AF647(orange) ,used under the same conditions. |
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文献和实验「CAR-T 之父」Carl June 引领抗癌风向标！挑战
RN7SL1，发现其可诱导 B16-F10 黑色素瘤细胞或人树突状细胞干扰素刺激基因（IFN-stimulated genes, ISGs）的表达，如主要组织相容性复合体 I 类（MHC I 类）、PDL1 和 CD86。为了确定 RN7SL1 和对照 RNA（Scrambled RNA, Scr RNA）在体内的效应，他们将 B16 肿瘤植入野生型小鼠的侧壁，并在瘤内注射脂质体包裹的 RNA。结果发现，瘤内注射 RN7SL1 促进肿瘤生长，并降低了小鼠体内的 CD4 和 CD8 T
D 的抗体，选择抗体时可优先挑选经过验证的，如有组织染色的结果更佳，像是 GeneTex 的兔单抗 GTX635362。GTX635362 RAS（G12D 突变体）抗体通过使用多种细胞及正常与癌组织对照进行正交策略验证；在人胰腺癌细胞系和 PDAC 小鼠模型的 RAS G12D 突变蛋白表现出高度的敏感性和特异性。左图为印迹（WB）和右图为免疫组化（IHC-P）。5. 蛋白过表达利用在细胞中过表达目标蛋白，并收取细胞裂解液后进行 WB 分析，理论上蛋白量增多，抗体辨识后的信号也会增强，因此可确认抗体
很强的染色效果，但也存在一些差异。AE 1 和KA 4 对皮肤和附件上皮、非角化性复层上皮、单层上皮（如胃肠道等）、泌尿系上皮、分泌性腺体的管道、间皮、乳腺和胸腺上皮可以有很强的染色，但不染正常的肝细胞、唾液腺和胰腺腺泡细胞以及肾小管上皮细胞。这些细胞优先表达角蛋白18，不能被AE 1 和KA 4 所识别。相反，CAM―5.2则能识别角蛋白18，可染肝细胞，腺泡细胞和肾小管上皮细胞，但不能染正常的表皮细胞。而AE
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